耐除草剂转基因玉米对田间节肢动物群落多样性的影响

doi:10.11934/j.issn.1673-4831.2018.04.006

摘要/Abstract

摘要：

为了明确转基因耐草甘膦/草铵膦玉米C0010.1.1对大田节肢动物群落多样性的影响，2015年在田间隔离条件下，采用直接观察法和陷阱法对大田节肢动物群落进行统计，分析比较了节肢动物的功能群、多样性指数、优势集中性指数、均匀性指数等相关参数，并计算节肢动物群落的相似度。与非转基因对照玉米178和常规玉米先玉335相比，转基因玉米C0010.1.1田间节肢动物物种数、多样性指数、均匀性指数和优势集中性指数等指标随时间动态变化的趋势基本一致，没有显著差异。这表明转基因玉米C0010.1.1田间节肢动物群落结构与受体品系和常规种基本相似，与对照相比该转基因玉米对田间节肢动物群落的影响无显著差异。

关键词: 玉米, 节肢动物群落, 生物多样性, 优势集中性指数, 均匀性指数

Abstract:

For assessing the effects of transgenic glyphosate tolerance and glufosinate-ammonium tolerance maize C0010.1.1 on biodiversity of arthropod community, which supplied basic data for the studies of transgenic crop biosafety, in the isolated field the number of species and individuals in the arthropod communities were counted and the functional groups, biodiversity index, dominance index, evenness index and the community similarity were analyzed and compared in 2015. Compared to the non-transgenic maize counterpart 178 and conventional line Xianyu 335, the parameters, such as the number of species, biodiversity index, dominance index and evenness index, were similar in the trend over time with no significant difference. These results suggest that the arthropod community structures in the fields of the transgenic maize C0010.1.1, the counterpart 178 and the conventional line Xianyu 335 were equivalent, and no significant effects of genetically modified maize on field arthropod community were found.

Key words: maize (Zea mays), arthropod community, biodiversity, dominance index, evenness index

中图分类号:

X592

何浩鹏, 任振涛, 沈文静, 刘标, 薛堃. 耐除草剂转基因玉米对田间节肢动物群落多样性的影响[J]. 生态与农村环境学报, 2018, 34(4): 333-341.

HE Hao-peng, REN Zhen-tao, SHEN Wen-jing, LIU Biao, XUE Kun. Effects of Transgenic Herbicide-Tolerate Maize on Biodiversity of Arthropod Communities in the Fields[J]. Journal of Ecology and Rural Environment, 2018, 34(4): 333-341.

参考文献

[1] 戈峰,欧阳芳,赵紫华.基于服务功能的昆虫生态调控理论[J].应用昆虫学报,2014,51(3):597-605.[GE Feng,OUYANG Fang,ZHAO Zi-hua.Ecological Management of Insects Based on Ecological Services at a Landscape Scale[J].Chinese Journal of Applied Entomology,2014,51(3):597-605.]
[2] JAMES C.2015年全球生物技术/转基因作物商业化发展态势[J].中国生物工程杂志,2016,36(4):1-11.[JAMES C.The Global Commercialization of Biotech Crops and Biotech Crop Highlights in 2015[J].China Biotechnology,2016,36(4):1-11.]
[3] LOSEY J E,RAYOR L S,CARTER M E.Transgenic Pollen Harms Monarch Larvae[J].Nature,1999,399(6733):214.
[4] WOLFENBARGER L L,PHIFER P R.The Ecological Risks and Benefits of Genetically Engineered Plants[J].Science,2000,290(5499):2088-2093.
[5] DALE P J,CLARKE B,FONTES E M G.Potential for the Environmental Impact of Transgenic Crops[J].Nature Biotechnology,2002,20:567-574.
[6] ANDOW D A,ZWAHLEN C.Assessing Environmental Risks of Transgenic Plants[J].Ecology Letters,2006,9(2):196-214.
[7] ROMEIS J,BARTSCH D,BIGLER F,et al.Assessment of Risk of Insect-Resistant Transgenic Crops to Nontarget Arthropods[J].Nature Biotechnology,2008,26:203-208.
[8] APHIS (Animal and Plant Health Inspection Service),USDA.Docket No.98-126-2:Availability of Determination of Nonregulated Status for Rice Genetically Engineered for Glufosinate Herbicide Tolerance[J].Federal Register,1999,64(80):22595.
[9] 黄芊,凌炎,蒋显斌,等.转Bar基因水稻及草铵膦对褐飞虱取食和产卵行为的影响[J].南方农业学报,2013,44(7):1110-1114.[HUANG Qian,LING Yan,JIANG Xian-bin,et al.Impacts of Transgenic Tice With Bar Gene and Glufosinate on Feeding and Oviposition Behavior of Brown Planthopper,Nilaparvata lugens(Stål)[J].Journal of Southern Agriculture,2013,44(7):1110-1114.]
[10] 蒋显斌,黄芊,凌炎.利用黑肩绿盲蝽兼性取食特性评价转基因水稻生态风险[J].中国生物防治学报,2016,32(3):311-317.[JIANG Xian-bin,HUANG Qian,LING Yan,et al.Ecological Risk Assessment of Transgenic Rice by Using the Facultative Feeding of Cyrtorhinus lividipennis[J].Chinese Journal of Biological Control,2016,32(3):311-317.]
[11] 谷荣辉,成功,李建钦,等.转基因水稻对土壤性质及土壤生物的影响[J].云南农业大学学报,2014,29(3):448-457.[GU Rong-hui,CHENG Gong,LI Jian-qin,et al.Effects of Transgenic Rice on Properties and Soil Organisms[J].Journal of Yunnan Agricultural University,2014,29(3):448-457.]
[12] BITZER R J,BUCKELEW L D,PEDIOGO L D.Effects of Transgenic Herbicide-Resistant Soybean Varieties and Systems on Surface-Active Springtails (Entognatha collembola)[J].Environmental Entomology,2002,31(3):449-461.
[13] MCPHERSON R M,JOHNSON W C,MULLINIX JR B G,et al.Influnce of Herbicide Tolerant Soybean Production Systems on Insect Pest-Induced Crop Damage[J].Journal Economic Entomology,2003,96(3):690-698.
[14] YU H L,LI Y H,LI X J,et al.Arthropod Abundance and Diversity in Transgenic Bt Soybean[J].Environmental Entomology,2014,43(4):1124-1134.
[15] VOLKMAR C,HUSSEIN L M,JANY D,et al.Ecological Studies on Epigeous Arthropod Populations of Transgenic Sugar Beet at Friemar (Thuringia, Germany)[J].Agriculture,Ecosystems & Enviormnent,2003,95(1):37-47.
[16] 韩兰芝,白树雄,赵建周,等.转基因抗虫棉花和玉米与节肢动物相关的生态安全性研究进展[J].昆虫学报,2007,50(7):727-736.[HAN Lan-zhi,BAI Shu-xiong,ZHOU Jian-zhou,et al.Progress in Ecological Biosafety of Insect-Resistant Transgenic Cotton and Corn in Relation to Arthropods[J].Acta Entomologica Sinica,2007,50(7):727-736.]
[17] DAVIS S.EPA Revokes Approval of Enlist Duo Herbicide to Study ‘Synergistic Effects’.[EB/OL].[2016-09-26] http://www.agripulse.com/EPA-revokes-approval-of-Enlist-Duo-herbicide-to-studysynergistic-effects-11252015.asp.
[18] MANN R K,MASTERS R A,MCMASTER S,et al.Synergistic Herbicidal Weed Control From Combinations of 2,4-D-Choline and Glyphosate[P].U.S.Patent Appl 0173371 A1,2015.
[19] 梁文举,闻大中.土壤生物及其对土壤生态学发展的影响[J].应用生态学报,2001,12(1):137-140.[LIANG Wen-ju,WEN Da-zhong.Soil Biota and Its Role in Soil Ecology.[J]Chinese Journal of Applied Ecology,2001,12(1):137-140.]
[20] 王子健,刘佳,王尚,等.净月潭国家森林公园凋落物层土壤动物群落多样性[J].生态与农村环境学报,2012,28(4):368-372.[WANG Zi-jian,LIU Jia,WANG Shang,et al.Community Diversity of Litter Invertebrates in Jingyuetan National Forest Park of Changchun,China[J].Journal of Ecology and Rural Environment,2012,28(4):368-372.]
[21] 吴奇,彭德良,彭于发.抗草甘膦转基因大豆对非靶标节肢动物群落多样性的影响[J].生态学报,2008,28(6):2622-2628.[WU Qi,PENG De-liang,PENG Yu-fa.Effects of Herbicide Tolerant Soybean on Biodiversity of Non-Target Arthropods Communities[J].Acta Ecologica Sinica,2008,28(6):2622-2628.]
[22] LU Y H,WU K M,JIANG Y Y,et al.Mirid Bug Outbreaks in Multiple Crops Correlated With Wide-Scale Adoption of Bt Cotton in China[J].Science,2010,328(5982):1151-1154.
[23] 雒珺瑜,刘传亮,张帅,等.转RRM2基因棉生长势和产量及对棉田节肢动物群落的影响[J].植物生态学报,2014,38(7):785-794.[LUO Jun-yu,LIU Chuan-liang,ZHANG Shuai,et al.Growth Vigour and Yield of Transgenic RRM2 (RNA Recognition Motif 2) Cotton and Their Effects on Arthropod Community in Cotton Field[J].Chinese Journal of Plant Ecology,2014,38(7):785-794.]
[24] 季香云,徐雪亮,蒋杰贤,等.转Bt基因水稻"赣绿1号"对田间节肢动物群落的影响[J].应用昆虫学报,2014,51(3):827-833.[JI Xiang-yun,XU Xue-liang,JIANG Jie-xian,et al.Impact of Transgenic Bt Rice "Ganly 1" on an Arthropod Community[J].Chinese Journal of Applied Entomology,2014,51(3):827-833.]
[25] CARPENTER J E.Impacts of GM Crops on Biodiversity[J].GM Crops,2011,2(1):7-23.
[26] BUCKELEW L D,PEDIGO L P,MERO H M,et al.Effects of Weed Management Systems on Canopy Insects in Herbicide-Resistant Soybeans[J].Journal of Economic Entomology,2000,93(5):1437-1443.
[27] PIERRE J,MARSAULT D,GENECQUE E,et al.Effects of Herbicide-Tolerant Transgenic Oilseed Rape Genotypes on Honey Bees and Other Pollinating Insects Under Field Conditions[J].Entomologia Experimentalis et Applicata,2003,108(3):159-168.
[28] BOHAN D A,BOFFEY C W H,BROOKS D R,et al.Effects on Weed and Invertebrate Abundance and Diversity of Herbicide Management in Genetically Modified Herbicide-Tolerant Winter-Sown Oilseed Rape[J].Proceedings of the Royal Society of London B:Biological Sciences,2005,272(1562):463-474.
[29] VOLKMAR C,HUSSEI L M,KREUTER T,et al.Does the Cultivation of Transgenic Sugar Beet Affect the Stability of Arthropod Coenoses (Results of a Four-Year Field Study)?[J].Journal of Plant Diseases and Protection,2002,109:1031-1038.
[30] STRANDBERG B,PEDERSEN M B,ELMEGAARD N.Weed and Arthropod Populations in Conventional and Genetically Modified Herbicide Tolerant Fodder Beet Fields[J].Agriculture,Ecosystems & Environment,2005,105(1/2):243-253.
[31] 蒋显斌,肖国樱.抗除草剂转基因水稻对二种刺吸性害虫和二种天敌的影响[J].昆虫知识,2010,47(6):1128-1133.[JIANG Xian-bin,XIAO Guo-ying.Effects of Genetically Modified Herbicide Tolerant Rice (Oryza sativa) on Population Density of Two Sucking Insect Pests and Two Natural Enemies in Rice Fields[J].Chinese Bulletin of Entomology,2010,47(6):1128-1133.]
[32] 蒋显斌,肖国樱.转基因抗除草剂水稻对稻田叶冠层节肢动物群落多样性的影响[J].中国生态农业学报,2010,18(6):1277-1283.[JIANG Xian-bin,XIAO Guo-ying.Diversity of Arthropod Community in the Canopy of Genetically Modified Herbicide-Tolerant Rice (Oryza sativa L.)[J].Chinese Journal of Eco-Agriculture,2010,18(6):1277-1283.]
[33] 蒋显斌,肖国樱.抗除草剂转基因水稻对稻纵卷叶螟田间自然种群的影响[J].植物保护,2011,37(2):50-54.[JIANG Xiao-bin,XIAO Guo-ying.Effects of Genetically Modified Herbicide-Tolerant Rice on Natural Populations of Rice Leaf Roller[J].Plant Protection,2011,37(2):50-54.]
[34] VOLKMAR C,HUSSEIN M,WETZEL T.Ecological Field Studies in Transgenic Maize at Friemar (Thuringia)[J].Journal of Plant Diseases and Protection,2004(19):1017-1024.
[35] HEARD M S,CLARK S J,ROTHERY P,et al.Effects of Successive Seasons of Genetically Modified Herbicide-Tolerant Maize Cropping on Weeds and Invertebrates[J].Annals of Applied Biology,2006,149(3):249-254.
[36] 王尚,王柏凤,严杜升,等.转EPSPS基因抗除草剂玉米CC-2对田间节肢动物多样性的影响[J].生物安全学报,2014,23(4):271-277.[WANG Shang,WANG Bai-feng,YAN Du-sheng,et al.Impacts of Transgenic Herbicide-Resistant Maize With EPSPS Gene on Arthropod Biodiversity in the Fields[J].Journal of Biosafety,2014,23(4):271-277.]
[37] 姜莹,王柏凤,周琳.转EPSPS基因抗除草剂玉米‘CC-2’对土壤动物群落的短期影响[J].植物保护,2017,43(1):34-39,45.[JIANG Ying,WANG Bai-feng,ZHOU Lin,et al.Impacts of Transgenic Herbicide-Resistant Maize With EPSPS Gene on Soil Fauna Community[J].Plant Protection,2017,43(1):34-39,45.]
[38] 陈亮,黄庆华,孟丽辉,等.转基因作物饲用安全性评价研究进展[J].中国农业科学,2015,48(6):1205-1218.[CHEN Liang,HUANG Qing-hua,MENG Li-hui,et al.Safety Evaluation of Feeds From Genetically Modified Crops on Livestock and Poultry[J].Scientia Agricultura Sinica,2015,48(6):1205-1218.]
[39] 刘玲,杨殿林,王生荣,等.转Bt基因玉米种植对土壤细菌数量及多样性的影响[J].生态与农村环境学报,2011,27(3):42-47.[LIU Ling,YANG Dian-lin,WANG Sheng-rong,et al.Effects of Transgenic Bt Maize on Populations and Diversity of Soil Bacteria[J].Journal of Ecology and Rural Environment,2011,27(3):42-47.]
[40] 尹艳宁,方志翔,沈文静,等.转cry1Ac+cry2Ab基因棉对土壤细菌群落结构和功能多样性的影响[J].生态与农村环境学报,2014,30(2):206-213.[YIN Yan-ning,FANG Zhi-xiang,SHEN Wen-jing,et al.Impacts of Transgenic cry1Ac+cry2Ab Cotton on Community Structure and Functional Diversity of Soil Bacteria[J].Journal of Ecology and Rural Environment,2014,30(2):206-213.]
[41] KOVACH J,PETZOLDT C,DEGNI J,et al.A Method to Measure the Environmental Impact of Pesticides[J].New York's Food and Life Sciences Bulletin,1992(139):8.
[42] BONNY S.Herbicide-Tolerant Transgenic Soybean Over 15 Years of Cultivation:Pesticide Use,Weed Resistance,and Some Economic Issues.The case of the USA[J].Sustainability,2011,3(9):1302-1322.
[43] BARFOOT P,BROOKES G.Key Global Environmental Impacts of Genetically Modified (GM) Crop Use 1996-2012[J].GM Crops & Food,2014,5(2),149-160.
[44] OLIVER D P,KOOKANA R S,MILLER R B,et al.Comparative Environmental Impact Assessment of Herbicides Used on Genetically Modified and Non-Genetically Modified Herbicide-Tolerant Canola Crops Using Two Risk Indicators[J].Science of the Total Environment,2016,557/558:754-763.