关键词: 鬼针草, 丛枝菌根, 煤矿, 重金属

Abstract: In order to better expand land reclamation and ecological reconstruction of wasteland of coal mine tailing in Mingshan, effect of inoculation of Bidens bipinnata (a kind of indigenous pioneer plant) with arbuscular mycorrhizal fungi on the plants absorbing soil heavy metal was studied in an experiment that was designed to have three ratios of mixture of yellow earth and coal gangue and four species of arbuscular mycorrhizal fungi, i.e. Glomus intraradices BGC BJ09 (A1), G. mosseae BGC NM01A (A2), G. mosseae BGC HEB07B (A3) and G. versiforme BGC GD01C (A4) for comparison. Results show that A4 is the most suitable inoculant for Bidens bipinnata , in terms of biomass of the host, mycorrhizal infection rate and intensity, spore density (per 50 g dry soil), and root activity. Compared with CK, A1 promoted Cd uptake of the plant; the 4 isthe 4 treatments are all insignificant in their effects on absorption or repulsion of Cu and Mn; A3 significantly promoted removal of Cu and Mn, while A4 did that of Mn.Biomass of the inoculated plants in treatments of 2 cm-thick overlaying yellow earth layer on 9 cm-thick coal gangue is higher than that of uninoculated plants in treatments of 7 cm-thick overlaying yellow earth layer on 4 cm -thick coal gangue, indicating that the inoculation has the potential of offsetting the effect of thin overlaying reducing biomass of the plants. It could, therefore, be concluded that the practice of arbuscular mycorrhizae inoculation may reduce the need for thick overlaying soil layer, and hence significantly save the cost of land reclamation.

Key words: Bidens bipinnata, arbuscular mycorrhiza(AM), coal mining, heavy metal