利用彗星试验研究菲对蚕豆DNA的损伤

生态与农村环境学报 ›› 2014, Vol. 30 ›› Issue (4): 515-520.doi:

利用彗星试验研究菲对蚕豆DNA的损伤

高曦,盛月慧,高彦征,陈则友,胡小婕

南京农业大学土壤有机污染控制与修复研究所

收稿日期:2013-11-07 修回日期:2014-06-18 出版日期:2014-07-25 发布日期:2014-10-11
通讯作者: 高彦征南京农业大学土壤有机污染控制与修复研究所 E-mail:gaoyanzheng@njau.edu.cn
作者简介:高曦（１９８７—），男，山东莱州人，硕士生，主要从事污染物基因毒性研究。E-mail:gaoxi_ix@163.com
基金资助:
国家自然科学基金（４１１７１１９３，４１１７１３８０）；江苏省科技支撑计划（ＢＥ２０１１７８０）

Analysis of Phenanthrene-Caused DNA Damage on the Root Tip Cells of Vicia faba With Comet Assay

GAO Xi, SHENG Yue-Hui, GAO Yan-Zheng, CHEN Ze-You, HU Xiao-Jie

Institute of Organic Contaminant Control and Soil Remediation,Nanjing Agricultural University

Received:2013-11-07 Revised:2014-06-18 Online:2014-07-25 Published:2014-10-11
Contact: GAO Yan-Zheng Institute of Organic Contaminant Control and Soil Remediation,Nanjing Agricultural University E-mail:gaoyanzheng@njau.edu.cn

摘要/Abstract

摘要： 以蚕豆(Vicia faba)为对象、菲为多环芳烃(PAHs)代表物开展彗星实验，用Komet Version 6.0软件进行彗星图像分析，并选择取评价参数研究PAHs污染的DNA损伤效应。以Olive尾动量(OTM)作为DNA损伤的评价参数，细胞彗星实验中蚕豆根尖DNA损伤的程度随菲质量浓度(0~0.6 mg·L^-1)升高而显著增大。最高浓度组(0.6 mg·L^-1OTM值比阴性对照增加132.63%，存在剂量依赖性，表明菲能诱导蚕豆根尖DNA的损伤。在培养液中菲质量浓度为0.1 mg·L^-1条件下培养蚕豆0~12h时DNA损伤随时间延长而增大，12h时OTM值为38.82±4.48；12~24h时DNA损伤则随时间延长而趋小。为确定菲污染导致DNA损伤的途径，进行非细胞彗星实验，结果表明，0.05 mg·L^-1菲即可造成显著DNA损伤(OTM值为22.27±0.42)，且DNA损伤程度随菲浓度提高而增大。菲对蚕豆根尖细胞可能存在直接的DNA损伤效应。

关键词: 菲, DNA损伤, 彗星试验, 蚕豆

Abstract: DNA damage of Vicia faba root tip cells caused by phenanthrene was investigated using the comet assay. The resulting comet image was analyzed using Software Komet Version 6.0. Olive tail moment (OTM) was found to be the most preferable parameter to evaluate DNA damages. As observed in the cellular comet assay, the DNA damage of Vicia faba root tip cells was aggravated with rising phenanthrene concentrations (0-0.6 mg•L^-1) in solution. With a high concentration (0.6 mg•L^-1), the OTM increased by 132.63% as compared with the control. An obvious phenanthrene dose-dependent relationship was observed for DNA damage. However, DNA damage by 0.1 mg•L^-1 phenanthrene was time-dependent, aggravating with the passing time in the first 12 h and then mitigating in the second 12 h. In order to determine pathways of phenanthrene-caused DNA damage, direct effect of phenanthrene on Vicia faba root tip cell DNA was assessed with the acellular comet assay. It was found that phenanthrene at 0.05 mg•L^-1 caused significant damage (OTM 22.27±0.42) to Vicia faba root tip cell DNA and the damage aggravated with the increasing phenanthrene concentration (0-0.6 mg•L^-1).

Key words: phenanthrene, DNA damage, comet assay, Vicia faba

中图分类号:

高曦, 盛月慧, 高彦征, 陈则友, 胡小婕. 利用彗星试验研究菲对蚕豆DNA的损伤[J]. 生态与农村环境学报, 2014, 30(4): 515-520.

GAO Xi, SHENG Yue-Hui, GAO Yan-Zheng, CHEN Ze-You, HU Xiao-Jie. Analysis of Phenanthrene-Caused DNA Damage on the Root Tip Cells of Vicia faba With Comet Assay[J]. Journal of Ecology and Rural Environment, 2014, 30(4): 515-520.

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