关键词: 威廉腔环蚓, ¹⁴C示踪, ¹⁴C—p353—NP, 生物富集, 生长抑制率

Abstract:

A laboratory experiment was conducted using ¹⁴Ctracer to investigate bioaccumulation of  ¹⁴C—p353—NP in Metaphire guillelmi and its influence on growth of the worm. Results show that after 96 days of exposure, the concentration of  ¹⁴C—p353—NP in Metaphire guillelmi was measured to be 4.45~6.79 mg·g^-1(wet weight)with BSAF being in the range of 0.95-1.89. In the ¹⁴C—p353—NP treated groups, earthworms exposed to ¹⁴C—p353—NP, 128、160 and 200 mg·kg^-1  in concentration were inhibited significantly in growth（P<0.05).Bioaccumulation of ¹⁴C—p353—NP in Metaphire guillelmi and its inhibitory effect on growth was closely related to concentration of  ¹⁴C—p353—NP in the soil and duration of the exposure and their interaction (P<0.001). It was found that ¹⁴C—p353—NP might keep on accumulating in Metaphire guillelmi, and inhibiting its growth till it reached up to its sublethal concentration (≤200 mg·kg^-1). The findings of the research may serve as basic data for assessment of bio-toxicity and ecological risk of p353—NP in soil.

Key words: Metaphire guillelmi, ¹⁴C-tracer, ¹⁴C—p353—NP, accumulation, growth inhibition rate