施用畜禽粪便有机肥土壤抗生素抗性基因污染状况

doi:10.11934/j.issn.1673-4831.2016.04.023

生态与农村环境学报 ›› 2016, Vol. 32 ›› Issue (4): 664-669.doi: 10.11934/j.issn.1673-4831.2016.04.023

施用畜禽粪便有机肥土壤抗生素抗性基因污染状况

张兰河¹, 王佳佳^1,2, 高敏², 仇天雷², 韩梅琳², 王旭明²

1. 东北电力大学化学工程学院, 吉林吉林 132012;
2. 北京农业生物技术研究中心, 北京 100097

收稿日期:2015-06-10 出版日期:2016-07-25 发布日期:2016-07-26
通讯作者: 王旭明 E-mail:wangxuming@baafs.net.cn E-mail:wangxuming@baafs.net.cn
作者简介:张兰河(1971-),男,黑龙江双鸭山人,教授,博士,主要从事环境生物技术方面的研究。E-mail:zhanglanhe@163.com
基金资助:
家禽产业技术体系北京市创新团队专项资金（CARS-PSTP）；北京市农林科学院科技创新能力建设专项（KJCX2014302）

Pollution of Antibiotics Resistant Genes in Farmland Amended With Livestock Organic Manure

ZHANG Lan-he¹, WANG Jia-jia^1,2, GAO Min², QIU Tian-lei², HAN Mei-lin², WANG Xu-ming²

1. College of Chemical Engineering, Northeast Dianli University, Jilin 132012, China;
2. Beijing Agro-Biotechnology Research Center, Beijing 100097, China

Received:2015-06-10 Online:2016-07-25 Published:2016-07-26

摘要/Abstract

摘要：

为研究施用畜禽粪便有机肥农田土壤中抗生素抗性基因的分布状况，采集北京地区9个长期施用有机肥蔬菜基地的温室和大田土壤，对土壤中的抗生素耐药菌和18种抗性基因进行检测分析。结果表明，温室土壤中四环素耐药菌占总菌数的比例显著高于大田土壤（P<0.05），但温室土壤中氨苄西林、磺胺甲恶唑和环丙沙星耐药菌占总菌数的比例均与大田土壤无显著性差异（P>0.05）。大田土壤和温室土壤中磺胺类抗性基因sulⅠ、sul2和四环素类抗性基因tetL的检出率均为100%。其他抗性基因，如四环素抗性基因tetA、tetA/P、tetC，红霉素抗性基因ermB，以及Ⅰ类整合子（intⅠ1），温室土壤的检出率均高于大田土壤，这可能与温室土壤有机肥施用量较大有关。

关键词: 土壤, 有机肥, 抗生素耐药菌, 抗生素抗性基因

Abstract:

In order to investigate distribution of antibiotics resistant genes (ARGs) in farmland soil amended with organic manure, soil samples from greenhouses and fields in nine vegetable production bases in the suburbs of Beijing were collected for analysis of antibiotics resistant bacteria and 18 types of ARGs. The analysis shows that the soil samples from greenhouses were higher than those from fields in proportion of tetracycline resistant bacteria to total bacteria in count (P<0.05), and little difference was found between the two groups of soil samples in proportion of ampicillin, sulfa methoxazole and ciprofloxacin resistant bacteria to total bacteria in count. Sulfanilamide resistant genes (sulⅠ and sul2) and tetracycline resistance genes (tetL) were detected in all the soil samples, whereas tetracycline resistant genes (tetA, tetA/P and tetC), erythromycin resistant genes (ermB), and I integron (intⅠ1) were detected with a higher detectable rate in greenhouse soil than in field soil, which may be associated with more organic manure applied to the former than to the latter.

Key words: soil, organic manure, antibiotics resistant bacteria, antibiotics resistant genes

中图分类号:

张兰河, 王佳佳, 高敏, 仇天雷, 韩梅琳, 王旭明. 施用畜禽粪便有机肥土壤抗生素抗性基因污染状况[J]. 生态与农村环境学报, 2016, 32(4): 664-669.

ZHANG Lan-he, WANG Jia-jia, GAO Min, QIU Tian-lei, HAN Mei-lin, WANG Xu-ming. Pollution of Antibiotics Resistant Genes in Farmland Amended With Livestock Organic Manure[J]. Journal of Ecology and Rural Environment, 2016, 32(4): 664-669.

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施用畜禽粪便有机肥土壤抗生素抗性基因污染状况

Pollution of Antibiotics Resistant Genes in Farmland Amended With Livestock Organic Manure

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