上海市崇明岛养殖场周边环境中氟喹诺酮类抗生素的含量特征

doi:10.11934/j.issn.1673-4831.2017.02.004

摘要/Abstract

摘要：

在上海崇明岛选取15个不同规模的猪场、禽场和奶牛场，分别采集粪肥以及距离养殖场50 m内的地表水、土壤和相应的蔬菜样品，利用液相色谱串联质谱（LC/MS-MS）技术进行氧氟沙星（OFL）、恩诺沙星（ENR）、环丙沙星（CIP）和诺氟沙星（NOR）4种氟喹诺酮类抗生素含量分析，以了解目前上海市崇明岛养殖场周边环境中氟喹诺酮类抗生素的含量水平特征。结果表明，上海市崇明岛养殖场粪肥中氟喹诺酮类抗生素总含量范围为9.00~950 μg·kg^-1，平均值为144 μg·kg^-1，4种抗生素的检出率均在70%以上。周边地表水中氟喹诺酮总含量为2.8~477.5 μg·L^-1，平均值为86.8 μg·L^-1，ENR的检出率为66.7%，OFL、CIP和NOR的检出率均为100%。土壤中氟喹诺酮类抗生素总含量范围为19.1~802 μg·kg^-1，平均值为144 μg·kg^-1，OFL、ENR和CIP的检出率均为100%，NOR的检出率为90.9%。蔬菜中氟喹诺酮类抗生素总含量为ND~110 μg·kg^-1，平均值为21.9 μg·kg^-1。OFL和CIP的检出率为73.3%，ENR和NOR的检出率为53.3%。养殖场周边环境中氟喹诺酮类抗生素含量均值为粪肥>土壤>周边地表水>蔬菜。对同种氟喹诺酮类抗生素在养殖场周边不同环境介质中含量的相关性分析表明，OFL在粪肥与地表水中的含量呈正相关关系，OFL、CIP、NOR在水样与蔬菜中的含量呈正相关关系。

关键词: 养殖场, 粪肥, 地表水, 土壤, 蔬菜, 氟喹诺酮

Abstract:

In order to determine residue levels of fluoroquinolone-type antibiotics in the surroundings of livestock farms in Chongming Island of Shanghai, a total of 15 livestock farms different in size and nature (pig farms, poultry farms, dairy farms) were selected as objects for the study and samples of livestock manure and surface water, soil and vegetable growing within the radius of 50 m of the farms were collected for analysis of contents of ofloxacin (OFL), enrofloxacin (ENR), ciprofloxacin (CIP), and norfloxacin (NOR) using the liquid chromatography tandem mass spectrometry (LC/MS-MS) method. Results show that in the livestock manure samples, total contents of the fluoroquinolone-type antibiotics varied in the range of 9.00-950 μg·kg^-1, with an average of 258 μg·kg^-1 and over 70% of the samples were found to contain the four types of antibiotics; in the surface water samples, total contents of the four types of antibiotics varied from 2.8 to 477.5 μg·kg^-1, with an average of 86.8 μg·kg^-1, and all the samples were found to have OFL, CIP and NOR and 66.7% to have ENR; in the soil samples, total contents of the four ranged from 19.1 to 802 μg·kg^-1, with an average of 144 μg·kg^-1, and all the samples were found to contain OFL, ENR and CIP, and 90.9% to contain NOR; and in the vegetable samples. total contents ranged from ND to 110 μg·kg^-1, with an average of 21.9 μg·kg^-1, and 73.3% of the samples were found to have OFL and CIP and 53.3% to have ENR and NOR. In terms of average content of fluoroquinolone-type of antibiotics the four sectors displayed an order of manure > soil > surface water > vegetable. Correlation analysis shows that the content of OFL in manure was positively related to that in surface water and the content of OFL, CIP and NOR in vegetables to their respective ones in surface water.

Key words: farms, manure, surface water, soil, vegetable, fluoroquinolones

中图分类号:

X592

赵晶, 毕春娟, 陈振楼, 施庆, 李林茹, 姚春霞. 上海市崇明岛养殖场周边环境中氟喹诺酮类抗生素的含量特征[J]. 生态与农村环境学报, 2017, 33(2): 120-126.

ZHAO Jing, BI Chun-juan, CHEN Zhen-lou, SHI Qing, LI Lin-ru, YAO Chun-xia. Contents of Fluoroquinolone-Type Antibiotics in the Surroundings of Livestock Farms in Chongming Island of Shanghai[J]. Journal of Ecology and Rural Environment, 2017, 33(2): 120-126.

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