原油降解细菌ODB01的筛选、鉴定及降解特性

doi:10.11934/j.issn.1673-4831.2017.05.012

生态与农村环境学报 ›› 2017, Vol. 33 ›› Issue (5): 466-473.doi: 10.11934/j.issn.1673-4831.2017.05.012

原油降解细菌ODB01的筛选、鉴定及降解特性

蓝慧^1,2,3, 王翀^1,2,3, 杨辉^1,2,3, 潘虎^2,4, 卢向阳^1,2,3, 田云^1,2,3

1. 湖南农业大学生物科学技术学院, 湖南长沙 410128;
2. 农业生物化学与生物转化湖南省高校重点实验室, 湖南长沙 410128;
3. 畜禽废弃物资源化利用湖南省工程实验室, 湖南长沙 410128;
4. 西藏自治区农牧科学院农业质量标准与检测研究所, 西藏拉萨 850032

收稿日期:2016-06-22 出版日期:2017-05-25 发布日期:2017-05-25
通讯作者: 卢向阳,E-mail:xiangyangcn@163.com;田云,E-mail:tianyun@hunau.edu.cn E-mail:xiangyangcn@163.com;tianyun@hunau.edu.cn
作者简介:蓝慧(1987-)男,江西赣州人,硕士生,主要从事环境微生物方面的研究。E-mail:664604602@qq.com
基金资助:
湖南省战略性新兴产业关键共性技术导向类项目（2015GK1017）；西藏自治区财政专项资金

Isolation, Identification and Degradation Characters of a Crude Oil-Degrading Strain ODB01

LAN Hui^1,2,3, WANG Chong^1,2,3, YANG Hui^1,2,3, PAN Hu^2,4, LU Xiang-yang^1,2,3, TIAN Yun^1,2,3

1. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;
2. Hunan Province University Key Laboratory for Agricultural Biochemistry and Biotransformation, Changsha 410128, China;
3. Hunan Engineering Laboratory for Resource Utilization of Animal Faeces Wastes, Changsha 410128, China;
4. Institute of Agricultural Product Quality Standard and Testing Research, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850032, China

Received:2016-06-22 Online:2017-05-25 Published:2017-05-25

摘要/Abstract

摘要：

从长庆油田被原油污染土壤中筛选出1株以原油为唯一碳源的菌株ODB01。经菌株生理生化特征及16S rDNA序列分析，鉴定为肠杆菌属（Enterobacter sp.）细菌；运用响应曲面法优化细菌ODB01对原油的降解条件为pH值为8.91，w（NaCl）为1.19%，油菌比为1∶4.12，温度为36.78℃，在该条件下细菌ODB01对原油的降解率为34.6%；分别添加w=0.05%土温80（Tween 80）和w=0.05%辛基苯基醚（Triton X-100）后，细菌ODB01对原油降解率分别达到42.5%和46.1%。结果表明，细菌ODB01对原油具有一定的降解能力，有望作为一种微生物修复剂进行开发。

关键词: 原油降解细菌, 鉴定, 响应曲面法, 表面活性剂

Abstract:

A crude oil-degrading bacteria ODB01 was isolated from crude oil-contaminated soil of Changqing Oilfield, capable of utilizing crude-oil as the sole source of carbon for growth, and identified as Enterobacter sp., based on physiological characteristics and the analysis of 16S rDNA gene sequence. The optimization of crude oil degradation condition was carried out by response surface methodology (Box-Behnken), the results are as follows:pH, 8.91; NaCl concentration, 1.19%; oil-bacteria liquid proportion, 1:4.12; temperature, 36.78℃, and degradation rate reaches to 34.6% under the optimum condition; In addition, adding to 0.05% Tween 80 or 0.05% Triton X-100, the degradation rates of crude oil reached to 42.5% and 46.1%, respectively. These results indicated that the bacterium ODB01 had the capability of biodegrading crude oil and it maybe developed as a remediation agent.

Key words: crude oil-degrading bacterium, identification, response surface methodology, surfactant

中图分类号:

X253

蓝慧, 王翀, 杨辉, 潘虎, 卢向阳, 田云. 原油降解细菌ODB01的筛选、鉴定及降解特性[J]. 生态与农村环境学报, 2017, 33(5): 466-473.

LAN Hui, WANG Chong, YANG Hui, PAN Hu, LU Xiang-yang, TIAN Yun. Isolation, Identification and Degradation Characters of a Crude Oil-Degrading Strain ODB01[J]. Journal of Ecology and Rural Environment, 2017, 33(5): 466-473.

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原油降解细菌ODB01的筛选、鉴定及降解特性

Isolation, Identification and Degradation Characters of a Crude Oil-Degrading Strain ODB01

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