A2/O工艺处理污水中雌激素活性和典型雌激素水平变化

doi:10.11934/j.issn.1673-4831.2017.06.012

生态与农村环境学报 ›› 2017, Vol. 33 ›› Issue (6): 571-576.doi: 10.11934/j.issn.1673-4831.2017.06.012

• 研究方法 • 上一篇

A²/O工艺处理污水中雌激素活性和典型雌激素水平变化

游猛, 张秋亚, 王晓昌, 马晓妍

西安建筑科技大学环境与市政工程学院/国家城市非传统水资源开发利用国际科技合作基地/西北水资源与环境生态教育部重点实验室/陕西省污水处理与资源化工程技术研究中心/陕西省环境工程重点实验室, 陕西西安 710055

收稿日期:2016-07-28 出版日期:2017-06-25 发布日期:2017-06-15
通讯作者: 马晓妍,E-mail:maxiaoyan@xauat.edu.cn E-mail:maxiaoyan@xauat.edu.cn
作者简介:游猛(1991-),男,湖北黄石人,硕士生,主要研究方向为污水及再生水生物毒性的预测与评价。E-mail:1150755126@qq.com
基金资助:
城区水污染控制和水环境功能提升技术研究项目（2014ZX07305-002-01）；国家自然科学基金（51508449）；中国博士后科学基金面上项目（2015M572531）

Activity of Estrogen and Contents of Typical Estrogenic Compounds in A²/O Treated Wastewater

YOU Meng, ZHANG Qiu-ya, WANG Xiao-chang, MA Xiao-yan

School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology/International Science & Technology Cooperation Center for Urban Alternative Water Resources Development/Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education/Engineering Technology Research Center for Wastewater Treatment and Reuse, Shaanxi Province/Key Laboratory of Environmental Engineering, Shaanxi Province, Xi'an 710055, China

Received:2016-07-28 Online:2017-06-25 Published:2017-06-15

摘要/Abstract

摘要：

针对水环境中内分泌干扰物低浓度、难去除和高危害的特点，为全面了解污水处理过程中雌激素水平和效应的变化情况，采用HPLC-UV-FLD分析了污水中4种固醇类雌激素的浓度水平，采用重组酵母菌雌激素筛检法（YES）评价了城市污水处理流程（A²/O）中污水的雌激素活性，同时采用酶联免疫吸附法（ELISA）检测污水处理过程中雌二醇（E2）的浓度水平。结果表明，出水中雌酮（E1）质量浓度最高，为87.97 ng·L^-1，雌二醇（E2）质量浓度最低，为5.50 ng·L^-1，固醇类雌激素水平去除率为88.83%~95.69%。A²/O系统对雌激素活性的去除率为73.33%，但出水仍具有一定的雌激素活性，雌激素当量EEQ为1.92 ng·L^-1。ELSIA检测得雌二醇（E2）质量浓度为10.01~82.82 ng·L^-1，去除率为88.23%，与YES法检测结果具有一致性。ELISA检测可以成为体外检测的一个重要补充。

关键词: A²/O工艺, 雌激素, 雌激素活性, 酶联免疫吸附法(ELISA), 去除率

Abstract:

As endocrine disrupting chemicals in the water environment is low in concentration, difficult to remove and highly hazardous, it is essential to get a full grasp of how estrogen in wastewater varies with the process of treatment in activity and concentration. To that end, samples of wastewater were collected from an urban wastewater treatment installation for analysis of concentrations and activities of 4 kinds of steroid estrogens, using the high performance liquid chromatography-UV detector-fluorescence detector (HPLC-UV-FLD) and the yeast estrogen screen (YES) method, separately. Moreover, enzyme linked immunosorbent assay (ELISA) was performed to determine concentrations of E2 in the wastewater under treatment. Results show that among the four kinds of estrogens detected in the effluent, E1 was the highest in concentration, reaching 87.97 ng·L^-1 and E2, the lowest being 5.50 ng·L^-1, which indicates that the steroid estrogen removal rate varied in the range of 88.83%-95.69%. The A²/O system was found to be able to lower activity of the estrogens by 73.33%. However, the estrogens in the effluent were still quite active with EEQ being 1.92 ng·L^-1. ELISA reveals that the concentration of E2 in the effluent varied in the range of 10.01-82.82 ng·L^-1, which indicates that the removal rate of E2 was 88.23%. Obviously ELISA and YES are quite consistent in determination. Therefore, it could be concluded that ELISA could be a workable supplement to in vitro assays of estrogens.

Key words: A²/O process, estrogens, estrogenicity, ELISA, removal rate

中图分类号:

X592

游猛, 张秋亚, 王晓昌, 马晓妍. A²/O工艺处理污水中雌激素活性和典型雌激素水平变化[J]. 生态与农村环境学报, 2017, 33(6): 571-576.

YOU Meng, ZHANG Qiu-ya, WANG Xiao-chang, MA Xiao-yan. Activity of Estrogen and Contents of Typical Estrogenic Compounds in A²/O Treated Wastewater[J]. Journal of Ecology and Rural Environment, 2017, 33(6): 571-576.

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A²/O工艺处理污水中雌激素活性和典型雌激素水平变化

Activity of Estrogen and Contents of Typical Estrogenic Compounds in A²/O Treated Wastewater

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