不同薄壳山核桃品种的根际土壤细菌菌群结构及多样性研究

doi:10.11934/j.issn.1673-4831.2017.09.007

生态与农村环境学报 ›› 2017, Vol. 33 ›› Issue (9): 816-821.doi: 10.11934/j.issn.1673-4831.2017.09.007

不同薄壳山核桃品种的根际土壤细菌菌群结构及多样性研究

生静雅¹, 朱海军², 刘广勤², 万青¹

1. 江苏省农业科学院休闲农业研究所, 江苏南京 210014;
2. 江苏省农业科学院果树研究所, 江苏南京 210014

收稿日期:2016-10-10 出版日期:2017-09-25 发布日期:2017-09-25
通讯作者: 万青,E-mail:oneqq_17@163.com E-mail:oneqq_17@163.com
作者简介:生静雅(1984-),女,江苏南京人,助理研究员,硕士,研究方向为薄壳山核桃等干果育种栽培研究。E-mail:shengjingya1984@126.com
基金资助:
2015年中央财政林业科技推广项目（[2015]TJS03）

Community Structure and Diversity of Soil Bacteria in Rhizospheres Under Different Varieties of Carya illinoensis.

SHENG Jing-ya¹, ZHU Hai-jun², LIU Guang-qin², WAN Qing¹

1. Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. Institute of Pomology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2016-10-10 Online:2017-09-25 Published:2017-09-25

摘要/Abstract

摘要：

选择江苏薄壳山核桃主产区波尼（BN）、马汉（MH）、威斯顿（WSD）和肖肖尼（XXN）这4个主要品种，利用Illumina Miseq高通量测序技术研究其根际土壤微生物多样性及群落结构组成。结果显示，4种薄壳山核桃品种间根际土壤细菌群落结构在门、属水平上相似，α多样性指数无显著差异。变形菌门、拟杆菌门和芽单胞菌门均为优势门类，假单胞菌属（1%）和Chitinophagaceae菌群（5%）在4个薄壳山核桃品种的根际土壤中丰度均较高。研究初步阐述了薄壳山核桃根际土壤微生物多样性及群落结构组成，为进一步研究薄壳山核桃的根际效应提供了理论依据。

关键词: 薄壳山核桃, 根际细菌, 多样性, 胡桃醌

Abstract:

The Miseq molecular technology was used to explore difference between 4 varieties of Carya illinoensis ("BN"、"MH"、"XXN" and "WSD") in community structure and diversity of soil bacteria in rhizosphere. It was found that the 4 varieties did not vary much in community structure and diversity of soil bacteria in rhizosphere at the phylum and genus levels or in α-diversity index. Proteobacteria, Bacteroidetes and Gemmatimonadete were dominant phyla. Pseudomonas (1%) and Chitinophagaceae (5%) were quite high in mean abundance in the rhizosphere of all the four varieties of Carya illinoensis, which might be attributed to the chemotaxis of juglone. This study has tentatively elaborated community structure and diversity of the soil bacteria in rhizosphere of the Carya illinoensis trees and may serve as theoretical basis for future studies on rhizosphere effect of Carya illinoensis.

Key words: Carya illinoensis, rhizosphere bacteria, diversity, juglone

中图分类号:

X825

生静雅, 朱海军, 刘广勤, 万青. 不同薄壳山核桃品种的根际土壤细菌菌群结构及多样性研究[J]. 生态与农村环境学报, 2017, 33(9): 816-821.

SHENG Jing-ya, ZHU Hai-jun, LIU Guang-qin, WAN Qing. Community Structure and Diversity of Soil Bacteria in Rhizospheres Under Different Varieties of Carya illinoensis.[J]. Journal of Ecology and Rural Environment, 2017, 33(9): 816-821.

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不同薄壳山核桃品种的根际土壤细菌菌群结构及多样性研究

Community Structure and Diversity of Soil Bacteria in Rhizospheres Under Different Varieties of Carya illinoensis.

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