Abstract: To explore whether the symbiont of Houttuynia cordata (0, 5%, 10% and 15% in plant area) and GIFT tilapia may enhance immunological effects of serumal immune factors of the fish at different phases via pond cultivation of the plants on floating beds, immunoglobulin M (IgM), epidermal growth factor (EGF); iterferon-γ (IFN-γ), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8); metallothionein (MT) and transferrin (TRF) of the fish was measured. Results show that the fish in the 10% group were significantly higher than those in any other groups in serumal EGF, IL-8, IL-10, TNF-α and TRF, and that the fish in the 5% group were significantly higher than those in any other groups in IgM and IFN-γ without showing any adverse effect on MT in the fish. Apparently, planting Houttuynia cordata up to 5% in area in the fish pond of GIFT tilapia can significantly enhance activities of serumal immune factors at different phases of the immune reaction.