Abstract: With luciferase being used as reporter gene, reporter gene plasmid with luciferase (pERE-TATA-Luc) was regulated by estrogen response element and estrogen receptor expression plasmid (rERa/pCI) was obtained. Using the transient co-transfection method, luciferase reporter gene plasmid and estrogen receptor expression plasmid were transfected into the kidney cells (CV-1) of an African monkey. Estradiol was used as CK for positive phenomenon. E₂ induced expression of luciferase significantly when it was 10^-10 mol·L^-1 in concentration and to the maximum when it was 10^-9 mol·L^-1 in concentration, and its EC₅₀ was 6.1×10^-11 mol·L^-1. Through validating sensitivity, efficacy and stability of the estradiol, diethylstilbestrol and genistein detecting systems, a stable sensitive estrogen receptor reporter gene cell line was established for laboratory. Based on the CV-1 receptor gene reporter assay, interferon activity of bisphenol A analogues type of estrogen was studied. In terms of estrogenic activity, a decreasing order of BPAF > BPF > BPB > BPA > BPZ > BPAP > BPS > BPP was found. All the findings demonstrate that the CV-1 cell receptor reporter gene assay is a rapid effective procedure for screening endocrine disrupting chemicals for estrogenic activity.