基于高通量测序的冬季太湖竺山湾浮游细菌和沉积物细菌群落结构和多样性研究

doi:10.11934/j.issn.1673-4831.2017.11.005

生态与农村环境学报 ›› 2017, Vol. 33 ›› Issue (11): 992-1000.doi: 10.11934/j.issn.1673-4831.2017.11.005

基于高通量测序的冬季太湖竺山湾浮游细菌和沉积物细菌群落结构和多样性研究

薛银刚^1,2, 江晓栋¹, 孙萌³, 刘菲¹, 滕加泉², 耿金菊⁴, 谢文理², 张皓²

1. 常州大学环境与安全工程学院, 江苏常州 213001;
2. 江苏省环境保护水环境生物监测重点实验室/常州市环境监测中心, 江苏常州 213001;
3. 长沙理工大学水利工程学院, 湖南长沙 410114;
4. 南京大学环境学院污染控制与资源化研究国家重点实验室, 江苏南京 210023

收稿日期:2017-07-20 出版日期:2017-11-25 发布日期:2017-11-24
通讯作者: 薛银刚,E-mail:yzxyg@126.com E-mail:yzxyg@126.com
作者简介:薛银刚(1981-),男,江苏常州人,高级工程师,博士,研究方向为生态毒理学和生物监测。E-mail:yzxyg@126.com
基金资助:
江苏省环境监测科研基金（1404，1320）

Structure and Diversity Profiles of Planktonic and Sediment Bacteria Communities in the Zhushan Bay of Lake Taihu in Winter Based on High-Throughput Sequencing

XUE Yin-gang^1,2, JIANG Xiao-dong¹, SUN Meng³, LIU Fei¹, TENG Jia-quan², GENG Jin-ju⁴, XIE Wen-li², ZHANG Hao²

1. School of Environmental and Safety Engineering, Changzhou University, Changzhou 213001, China;
2. Key Laboratory of Environmental Protection of Water Environment Biological Monitoring of Jiangsu Province/Changzhou Environmental Monitoring Center, Changzhou 213001, China;
3. School of Hydraulic Engineering, Changsha University of Science and Technology, Changsha 410114, China;
4. State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210023, China

Received:2017-07-20 Online:2017-11-25 Published:2017-11-24

摘要/Abstract

摘要：

为了探究太湖竺山湾浮游细菌及沉积物细菌群落的结构及多样性变化，考察细菌与环境因子之间的生态耦合关系，基于16S rRNA基因的高通量测序技术，研究冬季太湖竺山湾的浮游细菌和沉积物细菌群落结构和多样性，对相对丰度排名居前10位的优势浮游细菌属与pH值、溶解氧浓度、叶绿素a浓度、藻密度、总氮浓度和总磷浓度等环境因子进行冗余分析。结果表明，从门水平来看，水样和沉积物中蓝藻细菌门（Cyanobacteria）平均相对丰度均较高，分别为46.95%和22.19%；从纲水平来看，水样中的优势细菌主要为未分类的放线菌纲（unidentified Actinobacteria）和未分类的蓝藻细菌纲（unidentified Cyanobacteria），相对丰度分别为31.87%和30.46%，而沉积物中则主要为Chloroplast （14.34%）、γ-变形菌纲（Gammaproteobacteria，10.97%）和硝化螺旋菌纲（Nitrospira，9.89%）；从属水平来看，水样中微囊藻属（Microcystis）占优势，其次为hgcI clade，沉积物中微囊藻属和未分类的硝化螺旋菌属（unidentified Nitrospiraceae）占优势。通过研究发现水体中微囊藻属相对丰度最高，且与藻密度、溶解氧浓度和pH值呈正相关。

关键词: 太湖, 竺山湾, 细菌群落, 高通量测序, 环境因子, 微囊藻属

Abstract:

In order to explore changes in community structure and diversity of the planktonic and sediment bacteria in the Zhushan Bay of Lake Taihu, and to investigate relationships between bacteria and environmental factors, researches were carried out on community structure and diversity of planktonic and sediment bacteria in the Zhushan Bay of Lake Taihu in winter using the technique of 16S rRNA gene high-throughput sequencing, and relationships of the top ten genera of planktonic bacteria leading in dominancy with environmental factors (pH, dissolved oxygen concentration, chlorophyll a concentration, algal density, total nitrogen concentration, total phosphorus concentration, etc) through redundancy analysis. Results show that at the phylum level, Cyanobacteria is relatively higher in relative abundance in the water and sediment, reaching 46.95% and 22.19%, respectively, while at the class level, unidentified Actinobacteria and unidentified Cyanobacteria in the water, accounting for 31.87% and 30.46%, respectively, of the total, and Chloroplast, Gammaproteobacteria and Nitrospira in the sediment, making up 14.34%, 10.97% and 9.89% of the total, respectively, and at the genus level, Microcystis is the most dominant one in the water and followed by hgcI clade, and Microcystis and unidentified Nitrospiraceae are the dominant ones in the sediment. Through the study it is found that in the surface water, the genus of Microcystis is the highest in relative abundance, which is positively related to algal density, dissolved oxygen concentration and pH.

Key words: Lake Taihu, Zhushan Bay, bacterial community, high-throughput sequencing, environmental factor, Microcystis

中图分类号:

X832

薛银刚, 江晓栋, 孙萌, 刘菲, 滕加泉, 耿金菊, 谢文理, 张皓. 基于高通量测序的冬季太湖竺山湾浮游细菌和沉积物细菌群落结构和多样性研究[J]. 生态与农村环境学报, 2017, 33(11): 992-1000.

XUE Yin-gang, JIANG Xiao-dong, SUN Meng, LIU Fei, TENG Jia-quan, GENG Jin-ju, XIE Wen-li, ZHANG Hao. Structure and Diversity Profiles of Planktonic and Sediment Bacteria Communities in the Zhushan Bay of Lake Taihu in Winter Based on High-Throughput Sequencing[J]. Journal of Ecology and Rural Environment, 2017, 33(11): 992-1000.

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基于高通量测序的冬季太湖竺山湾浮游细菌和沉积物细菌群落结构和多样性研究

Structure and Diversity Profiles of Planktonic and Sediment Bacteria Communities in the Zhushan Bay of Lake Taihu in Winter Based on High-Throughput Sequencing

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