3株芽孢杆菌在水稻根际定殖促生及其在土壤中的存活

doi:10.19741/j.issn.1673-4831.2018.0692

摘要/Abstract

摘要：

植物根际促生菌（PGPR）可分泌植物生长激素，促进土壤养分循环，是生物肥料重要的种质资源。PGPR吸附于作物根系是形成定殖与功能的必要条件，抗性药物标记是研究功能菌株定殖促生能力的一种手段。利用解钾功能菌（Bacillus aryabhattai LZP01，简称LZP01）、解磷功能菌（Bacillus pumilus LZP02，简称LZP02）和溶磷功能菌（Bacillus megaterium LZP03，简称LZP03）这3株高效的水稻根际促生菌，通过抗生素药物利福平梯度筛选分别得到标记菌株K-LZP01、K-LZP02和K-LZP03，并且研究标记菌株的功能保留情况，进而检测标记菌株在水稻根际与土壤中定殖存活及促生能力。结果表明，水稻幼根浸入菌液后吸附量随浸入时间的增加而增大，其中K-LZP01在10min后吸附基本稳定；K-LZP01在50 min时吸附量达到最大；K-LZP03在5 min时吸附量达到最大，最大吸附量为12×10₇ CFU·g_-1，之后趋于动态稳定。将水稻幼苗浸入不同浓度菌悬液中，幼根吸附量随菌悬液浓度的增大而增大。标记菌株在接种于土壤中10 d后定殖量趋于稳定，且定殖能力较强。距离水稻根际越近标记菌株含量越高，表明菌株定殖竞争力越强。在灭菌土与未灭菌土条件下，3株菌株均具有促进水稻根系生长的能力。

关键词: 芽孢杆菌, 抗性标记, 定殖, 吸附, 水稻

Abstract:

Plant growth-promoting rhizobacteria (PGPR) can secrete plant growth hormone and promote soil nutrient cir-culation,which is an important germplasm resource of biological fertilizers. Adsorption of PGPR to crop roots is a neces-sary condition for colonization and function,and drug resistance marker is a mean of studying the ability of functional strains to colonize and promote growth. The purpose of the experiment is to label the strain by the antibiotic drug rifampi-cin,and to study the retention of the function of the strain,and then to determine the colonization survival ability of the strain in the rhizosphere of rice. The results show that the adsorption of rice roots to strain increased with the immersion time,and the adsorption content of K-LZP01 was basically stable after dipping for 10 min. K-LZP02 reached the maximum adsorption capacity at 50 min. The maximum adsorption capacity of K-LZP03 was 12×10₇ CFU·g_-1 at 5 min,and then be-came stable. When the rice seedlings were dipped in different concentrations of bacterial suspension, the adsorption capac-ity of radicle increased with increasing of bacterial suspension concentration. After 10 days of inoculation in soil,the colo-nization quantity of the labeled strains tended to be stable and the colonization ability is strong. The results found that the closer the distance to the rice rhizosphere,the higher the strain content,which indicate that the strains had good coloniza-tion competitiveness. In both sterilized and unsterilized soils,the three strains could all promote the growth of rice. In this study,researches on the resistance marker of PGPR strain as well as its colonization and growth promotion in rice rhizo-sphere were carried out,and the results are important to interpret the mechanism of PGPR and guide the production prac-tice.

Key words: Bacillus, resistance marker, colonization, adsorption, rice

中图分类号:

X172
Q93

王恒煦, 刘泽平, 王志刚, 徐伟慧, 郭茹鑫. 3株芽孢杆菌在水稻根际定殖促生及其在土壤中的存活[J]. 生态与农村环境学报, 2019, 35(7): 892-899.

WANG Heng-xu, LIU Ze-ping, WANG Zhi-gang, XU Wei-hui, GUO Ru-xin. Colonization and Growth Promotion of Three Bacillus Strains in Rice Rhizosphere and Their Survival in Soil[J]. Journal of Ecology and Rural Environment, 2019, 35(7): 892-899.

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