固定化Acinetobacter sp.T1细菌处理河道氨氮污染的研究

doi:10.19741/j.issn.1673-4831.2019.0169

生态与农村环境学报 ›› 2019, Vol. 35 ›› Issue (10): 1338-1345.doi: 10.19741/j.issn.1673-4831.2019.0169

固定化Acinetobacter sp.T1细菌处理河道氨氮污染的研究

肖尧, 吴晨捷, 蒋庆肯, 蓝际荣, 邬建勋, 孙燕, 杜冬云

中南民族大学资源与环境学院, 湖北武汉 430074

收稿日期:2019-03-21 出版日期:2019-10-25 发布日期:2019-10-23
通讯作者: 吴晨捷 E-mail:cjscun@mail.scuec.edu.cn
作者简介:肖尧(1991-),男(羌族),四川广元人,硕士生,主要从事水环境修复与水污染治理研究。E-mail:8150526@163.com
基金资助:
国家科技支撑计划（2015BAB01B03）；中央高校基本科研业务费专项资金（CZD18010）；中央高校基本科研业务专项资金（CZQ18021）

Study on the Treatment of Urban Polluted Water by Immobilized Acinetobacter sp. T1 Bacteria

XIAO Yao, WU Chen-jie, JIANG Qing-ken, LAN Ji-rong, WU Jian-xun, SUN Yan, DU Dong-yun

College of Resources and Environmental Science, South-Central University for Nationalities, Wuhan 430074, China

Received:2019-03-21 Online:2019-10-25 Published:2019-10-23

摘要/Abstract

摘要： 利用吸附固定在无机载体上的高效脱氮细菌Acinetobacter sp.T1去除城市河道及底泥中的氨氮。从沸石、活性炭和硅藻土中选出最佳固定载体，优化固定过程中的环境条件，并进行去除氨氮的模拟实验。结果表明：硅藻土因其具有较大的比表面积、较高的Zeta电位，能固定更多的细菌，因而以硅藻土为载体制备的固定化细菌具有更高的氨氮去除率。同时，细菌T1在温度为35℃、pH值为8、摇床转速为90 r·min^-1、固定时间为24 h的条件下，在硅藻土上固定化效果最佳。在去除氨氮的模拟实验中，曝气辅助固定化细菌组对水体及底泥中氨氮去除效果最佳，反应16 d水体COD、NH₄⁺-N和TN浓度分别减少75.98%、99.32%和89.36%，底泥TOC和TN含量分别减少54.56%和44.84%，底泥体积减少14.93%。

关键词: 固定化细菌, 底泥, 氨氮, 降解

Abstract: Ammonia removal in urban polluted water and sediments was achieved by immobilized high-efficiency nitrogen-removing bacteria strain Acinetobacter sp. T1 using inorganic carrier materials. Zeolite, activated carbon and diatomite were compared so as to find the best carrier material. The preferred environmental conditions (temperature, pH, rotation speed, fixation time) for immobilizing bacteria T1 were studied and the performance of the immobilized bacteria under optimal condition for ammonia removal were tested by batch scale simulation experiments. Results show that due to its large specific surface area and high zeta potential, more bacteria were fixed onto diatomite. The immobilized bacteria using diatomite as carrier showed higher ammonia nitrogen removal efficiency. The optimal conditions for the immobilization of bacterial T1 were as follows:temperature of 35℃, pH of 8, shaking speed of 90 r·min^-1 and fixation time of 24 h. Aeration-assisted immobilized bacteria experiment group showed highest ammonia removal efficiency during the simulation experiments. After 16 days of reaction, the COD, NH₄⁺-N and TN concentrations in water decreased by 75.98%, 99.32% and 89.36%, respectively. The sediment TOC and TN contents decreased by 54.56% and 44.84%, respectively, and the sediment volume decreased by 14.93%.

Key words: immobilized bacteria, sediment, ammonia nitrogen, degradation

中图分类号:

X524

肖尧, 吴晨捷, 蒋庆肯, 蓝际荣, 邬建勋, 孙燕, 杜冬云. 固定化Acinetobacter sp.T1细菌处理河道氨氮污染的研究[J]. 生态与农村环境学报, 2019, 35(10): 1338-1345.

XIAO Yao, WU Chen-jie, JIANG Qing-ken, LAN Ji-rong, WU Jian-xun, SUN Yan, DU Dong-yun. Study on the Treatment of Urban Polluted Water by Immobilized Acinetobacter sp. T1 Bacteria[J]. Journal of Ecology and Rural Environment, 2019, 35(10): 1338-1345.

参考文献 15

[1]	徐熊鲲,谢翼飞,陈政阳,等.曝气强化微生物功能菌修复黑臭水体[J].环境工程学报,2017,11(8):4559-4565.[XU Xiong-kun,XIE Yi-fei,CHEN Zheng-yang,et al.Remediation of Urban Black-Odorous River by Aeration Enhancing Functional Strains[J].Chinese Journal of Environmental Engineering,2017,11(8):4559-4565.]
[2]	戴兴宜.利用复合氮循环微生物消除富营养化湖泊底泥内源氮污染的研究[D].武汉:华中农业大学,2012.[DAI Xing-yi.The Research on Microbial Removal of Internal Nitrogen Pollution in Eutrophic Lake Sediments[D].Wuhan:Huazhong Agricultural University,2012.]
[3]	姚晓丽.微生物技术治理纳污塘及改善河道水质的研究[D].武汉:华中农业大学,2006.[YAO Xiao-li.Treating Pollutants-Receiving Ponds and Improving Water Quality in Rivers by Microbial Technology[D].Wuhan:Huazhong Agricultural University,2006.]
[4]	DING A Z,ZHANG Z H,FU J M,et al.Biological Control of Leachate From Municipal Landfills[J].Chemosphere,2001,44(1):1-8.
[5]	AOYAGI R,SATO R,TERADA A,et al.Novel Composite Gel Beads for the Immobilization of Ammonia-Oxidizing Bacteria:Fabrication,Characterization,and Biokinetic Analysis[J].Chemical Engineering Journal,2018,342:260-265.
[6]	吴光前,刘倩灵,周培国,等.固定化微生物技术净化黑臭水体和底泥技术[J].水处理技术,2008,34(6):26-29.[WU Guang-qian,LIU Qian-ling,ZHOU Pei-guo,et al.Remediation of Polluted Water and Sediment by Using Immobilized Microorganism Technology[J].Technology of Water Treatment,2008,34(6):26-29.]
[7]	LI C N,YANG J S,WANG X,et al.Removal of Nitrogen by Heterotrophic Nitrification-Aerobic Denitrification of a Phosphate Accumulating Bacterium Pseudomonas Stutzeri YG-24[J].Bioresource Technology,2015,182:18-25.
[8]	杜勇.生物炭固定化微生物去除水中苯酚的研究[D].重庆:重庆大学,2012.[DU Yong.Study of Biochar Immobilized Bacteria and Its Phenol Removal[D].Chongqing:Chongqing University,2012.]
[9]	何延青,刘俊良,杨平,等.微生物固定化技术与载体结构的研究[J].环境科学,2004,25(增刊1):101-104.[HE Yan-qing,LIU Jun-liang,YANG Ping,et al.Immobilization Technology and Construction of Carrier[J].Environmental Science,2004,25(Suppl.1):101-104.]
[10]	于鑫,张晓键,王占生.饮用水生物处理中生物量的脂磷法测定[J].给水排水,2002,38(5):1-5.[YU Xin,ZHANG Xiao-jian,WANG Zhan-sheng.Biomass Examination by Lipid-P Method for Drinking Water Bio-Treatment[J].Water & Wastewater Engineering,2002,38(5):1-5.]
[11]	李方舟,张琼,彭永臻.低DO硝化耦合内碳源反硝化脱氮处理生活污水[J].中国环境科学,2019,39(4):1525-1532.[LI Fang-zhou,ZHANG Qiong,PENG Yong-zhen.Treatment of Domestic Wastewater by Low DO Nitrification Coupled With Endogenous Denitrification System[J].China Environmental Science,2019,39(4):1525-1532.]
[12]	瞿艳芝,刘操,廖日红,等.固定化微生物技术处理城市微污染河水研究[J].环境科学,2009,30(11):3306-3310.[QU Yan-zhi,LIU Cao,LIAO Ri-hong,et al.Treatment of Micro-Polluted River Water by Immobilized Microorganism Technique[J].Environmental Science,2009,30(11):3306-3310.]
[13]	杜聪,冯胜,张毅敏,等.微生物菌剂对黑臭水体水质改善及生物多样性修复效果研究[J].环境工程,2018,36(8):1-7.[DU Cong,FENG Sheng,ZHANG Yi-min,et al.Study on the Improvement of Water Quality and Biological Diversity of Black and Odorous Water by Microbial Inoculants[J].Environmental Engineering,2018,36(8):1-7.]
[14]	DATTA S,CHRISTENA L R,RAJARAM Y R S.Enzyme Immobilization:An Overview on Techniques and Support Materials[J].Biotech,2013,3(1):1-9.
[15]	DI BONAVENTURA G,PICCOLOMINI R,PALUDI D,et al.Influence of Temperature on Biofilm Formation by Listeria Monocytogenes on Various Food-Contact Surfaces:Relationship With Motility and Cell Surface Hydrophobicity[J].Journal of Applied Microbiology,2008,104(6):1552-1561.

固定化Acinetobacter sp.T1细菌处理河道氨氮污染的研究

Study on the Treatment of Urban Polluted Water by Immobilized Acinetobacter sp. T1 Bacteria

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 15

相关文章 15

编辑推荐

Metrics

本文评价

[1]	郭铃, 方文婉, 葛峰, 戴亦军. 暗灰链霉菌CGMCC 13662降解苯甲腈和苯甲酸的研究[J]. 生态与农村环境学报, 2020, 36(2): 257-264.
[2]	杨欣妍, 魏儒平, 杨柳燕. 污染底泥制备陶粒及除磷潜力研究[J]. 生态与农村环境学报, 2019, 35(3): 368-376.
[3]	郭静静, 郭磊磊, 赵云岫, 葛峰, 戴亦军. 固氮菌(Ensifer meliloti 1021)降解苯甲腈及其代谢酶的基因克隆和表达[J]. 生态与农村环境学报, 2019, 35(2): 248-254.
[4]	刘海涛, 汪承润, 刘玲, 柳云云, 叶蕴, 罗康康. 羧基化多壁碳纳米管与铅镉对蚕豆幼苗叶片的氧化损伤[J]. 生态与农村环境学报, 2018, 34(8): 745-754.
[5]	李会琳, 何燕, 张静, 王岚, 杨宏, 彭正松, 路璐. 几种多环芳烃在不同类型稻田土壤中的降解行为[J]. 生态与农村环境学报, 2018, 34(7): 651-658.
[6]	高博, 刘彬, 王新, 赵洪锐, 张玉莹, 牛华英, 王景成. 半导体材料联合超声用于降解有机污染物研究进展[J]. 生态与农村环境学报, 2018, 34(6): 481-488.
[7]	卢俊平, 贾永芹, 张晓晶, 马太玲, 梅小乐, 杨红. 上覆水环境变化对底泥释氮强度影响模拟研究[J]. 生态与农村环境学报, 2018, 34(10): 924-929.
[8]	季丽, 宋超, 张聪, 陈家长, 胡庚东, 孟顺龙, 范立民, 裘丽萍, 郑尧, 刘颖, 吴伟. 化学氧化剂-微生物协同作用对除草剂氟乐灵降解过程的影响[J]. 生态与农村环境学报, 2017, 33(8): 743-747.
[9]	林琳, 万金忠, 李群, 周艳, 吴娟, 李川, 张胜田, 朱丽珺. 生物炭负载纳米零价铁材料的制备及还原降解性能[J]. 生态与农村环境学报, 2017, 33(7): 660-664.
[10]	吴晓妮, 付登高, 段昌群. 柴河流域不同农田种植模式下沟渠底泥氮磷及有机质的赋存特征[J]. 生态与农村环境学报, 2017, 33(6): 519-524.
[11]	蓝慧, 王翀, 杨辉, 潘虎, 卢向阳, 田云. 原油降解细菌ODB01的筛选、鉴定及降解特性[J]. 生态与农村环境学报, 2017, 33(5): 466-473.
[12]	郭敏, 吴文铸, 张圣虎, 宋宁慧, 石利利. 2种甲氧基丙烯酸酯类杀菌剂在土壤中的降解吸附特性及对地下水的影响[J]. 生态与农村环境学报, 2017, 33(5): 460-465.
[13]	芮胜阳, 吴娟, 崔娜欣, 李柱, 孔令为, 成水平. 底泥氧化还原环境对苦草（Vallisneria natans）生理生态及重金属元素摄取的影响[J]. 生态与农村环境学报, 2017, 33(3): 260-264.
[14]	张可, 吴艺琪, 陈伟, 格桑, 陈佳, 罗鸿兵. 施氏假单胞菌PFS-4的筛选、优化固定及其对二氯喹啉酸的降解性能[J]. 生态与农村环境学报, 2016, 32(6): 1018-1023.
[15]	吴文铸, 郭敏, 孔德洋, 许静, 单正军. 3种三唑类杀菌剂的环境降解特性[J]. 生态与农村环境学报, 2016, 32(5): 837-841.