腾格里沙漠沙坡头地区土壤微生物多样性分析

doi:10.11934/j.issn.1673-4831.2016.05.014

Abstract

Abstract:

Community composition, abundance and diversity of soil microbe in different vegetation rhizospheres and their responses to desert extreme environments in an artificial sand fixed area and a natural bare sand area in the Shapotou Desert, were studied. In the study, soil total DNA was extracted with the aid of the E.Z.N.A.^® Soil DNA Kit, V4-V5 sections of the 16S rDNA of the soil bacterial community analyzed with a MiSeq pyrosequencer for community composition, abundance and diversity of the soil bacteria, and responses of the soil microbial community in structure to changes in environment explained with the NMDS and Venn diagrams. In the soil samples, no matter from which area, Proteobacteria, Actinobacteria, Acidobacteria and Cyanobacteria were dominant groups in the soil microbial community on the phylum level, while the soil samples from the two different areas differed sharply in dormancy of groups on the genus level. The 11 soil samples were divided into two groups along the vertical axis of the NMDS in accordance with the artificial sand fixed area and the natural bare sand area, indicating that the microbial community responded to changes in the environment, i.e. sand fixation. The Venn diagram analysis indicates that although some differences are found between different soil samples,groups of microbes common in the two groups of soil samples exist and are thought to play a core role in the Shapotou Desert ecosystem. Cyanobacteria and Rhizobia are the two species of soil microbes that play an important role in biogeochemical cycling, like providing nutrients to psammophytes in sand-fixing process.

Key words: microbial community, extreme environment, Shapotou Desert, MiSeq sequencing

CLC Number:

X172
Q93

LI Jing-yu, ZHANG Xiu, SUN Min, ZHANG Yan-ling. Analysis of Soil Microbial Diversity in Shapotou Area of Tengger Desert[J]. Journal of Ecology and Rural Environment, 2016, 32(5): 780-787.

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Analysis of Soil Microbial Diversity in Shapotou Area of Tengger Desert

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