不同施肥模式和地下水位条件下红壤水稻土PLFA指纹特征

doi:10.11934/j.issn.1673-4831.2017.06.007

Abstract

Abstract:

Soil microbial community is an important indicator for assessing changes in soil quality, fertility and soil environment. Fertilization and groundwater table are two factors that may affect soil fertility and micro-environment. However, so far little has been reported on influence of groundwater table on soil microbial community, especially the joint effects of fertilization and groundwater table on soil microbial community. Phospholipid fatty acids (PLFA) analysis was conducted to evaluate impacts of fertilization and groundwater table on the soil microbial community in a red soil rice paddy. Results show that groundwater table significantly influenced soil pH, E_h,p (oxido-reduction potential in drained soil) and NO₃^- content, while soil fertilization did significantly soil E_h,y (oxido-reduction potential in flooded soil), E_h,p, total organic carbon, total N and available K contents. Besides, groundwater table significantly affected contents of total PLFA, bacterial PLFA, and Gram-positive (G⁺) bacterial PLFA and G⁺ PLFA/G^- PLFA ratio while fertilization did significantly content of Gram-negative (G^-) bacterial PLFA contents and G⁺ PLFA/G^- PLFA ratio of the soil microbial community. Stepwise regression analysis shows that soil pH and total nitrogen content significantly affected actinomycete PLFA content, and soil pH did significantly contents of G⁺ bacterial PLFA, aerobic and anaerobic bacterial PLFA, too; soil E_h,ydid significantly fungal to bacterial PLFA ratio; and contents of NO₃^--N and total K nitrogen did significantly G⁺ PLFA/G^- PLFA ratio. PCA analysis shows that the environmental factors significantly affected soil microbial structure, total K, pH and E_h,y of the soils under long-term fertilization and varying groundwater table, thus making Treatment LNOM different from the other treatments, while NO₃^--N, total N and total P contents were the important factors making Treatments HNOM, HHOM, LCF, HCF and LHOM different from each other in microbial community. Obviously long-term fertilization and groundwater table significantly affect physiochemical properties and soil microbial community in the soil.

Key words: fertilization, groundwater table, phospholipid fatty acids, microbial diversity

CLC Number:

S154.3
S182

ZHANG Yi-fei, XU Ting-ting, HAN Cheng, DENG Huan, YIN Li-chu, ZHONG Wen-hui. PLFA Fingerprint of Red Paddy Soils as Affected by Fertilization Pattern and Groundwater Table[J]. Journal of Ecology and Rural Environment, 2017, 33(6): 533-538.

References

[1] LOU Y S,LI Z P,ZHANG T L,et al.Carbon Dioxide Flux in a Subtropical Agricultural Soil of China[J].Water,Air,and Soil Pollution,2003,149(1):281-293.
[2] 张桃林.中国红壤退化机制与防治[M].北京:中国农业出版社,1999:138.[ZHANG Tao-lin.Mechanism and Control of Red Soil Degradation in China[M].Beijing:China Agriculture Press,1999:138.]
[3] 孙波,张桃林,赵其国.我国东南丘陵区土壤肥力的综合评价[J].土壤学报,1995,32(4):362-369.[SUN Bo,ZHANG Tao-lin,ZHAO Qi-guo.Comprehensive Evaluation of Soil Fertility in the Hilly and Mountainous Region of Southeastern China[J].Acta Pedologica Sinica,1995,32(4):362-369.]
[4] HUSSAIN Q,LIU Y Z,ZHANG A F,et al.Variation of Bacterial and Fungal Community Structures in the Rhizosphere of Hybrid and Standard Rice Cultivars and Linkage to CO₂ Flux[J].FEMS Microbiology Ecology,2011,78(1):116-128.
[5] STATUS C F Ⅲ,MCFARLAND J,DAVID M A,et al.The Changing Global Crabon Cycle Linking Plant-Soil Carbon Dynamics to Global Consequences[J].Journal of Ecology,2009,97(5):840-850.
[6] 滕应,黄昌勇,龙健,等.复垦红壤牧草根际微生物群落功能多样性[J].中国环境科学,2003,23(3):295-299.[TENG Ying,HUANG Chang-yong,LONG Jian,et al.Functional Diversity of Microbial Community in Herbage Rhizosphere of Reclaimed Red Soils[J].China Environmental Science,2003,23(3):295-299.]
[7] 颜慧,钟文辉,李忠佩,等.长期施肥对红壤水稻土磷脂脂肪酸特性和酶活性的影响[J].应用生态学报,2008,19(1):71-75.[YAN Hui,ZHONG Wen-hui,LI Zhong-pei,et al.Effects of Long-Term Fertilization on Phospholipid Fatty Acids and Enzyme Activities in Paddy Red Soil[J].Chinese Journal of Applied Ecology,2008,19(1):71-75.]
[8] 易亚男,尹力初,张蕾,等.施肥对不同地下水位水稻土团聚体组成及有机碳分布的影响[J].水土保持学报,2013,27(5):144-148.[YI Ya-nan,YIN Li-chu,ZHANG Lei,et al.Effects of Fertilization on Aggregate Composition and Organic Carbon Distribution in Paddy Soil Under Different Groundwater Level[J].Journal of Soil and Water Conservation,2013,27(5):144-148.]
[9] MARSCHNER P,KANDELER E,MARSCHNER B.Structure and Function of the Soil Microbial Community in a Long-Term Fertilizer Experiment[J].Soil Biology and Biochemistry,2003,35(3):453-461.
[10] FIERER N,JACKSON R B.The Diversity and Biogeography of Soil Bacterial Communities[J].Proceedings of the National Academy of Sciences of the United States of America,2006,103(3):626-631.
[11] 鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000:12-195.[LU Ru-kun.Soil Agricultural Chemical Analysis Method[M].Beijing:China Agriculture Scientech Press,2000:12-195.]
[12] BLIGH E G,DYER W J.A Rapid Method of Total Lipid Extraction and Purification[J].Canadian Journal of Biochemistry and Physiology,1959,37(8):911-917.
[13] ZHONG W H,GU T,WANG W,et al.The Effects of Mineral Fertilizer and Organic Manure on Soil Microbial Community and Diversity[J].Plant and Soil,2010,326(1):511-522.
[14] ZELLES L.Fatty Acid Patterns of Phospholipids and Lipopolysaccharides in the Characterisation of Microbial Communities in Soil:A Review[J].Biology and Fertility of Soils,1999,29(2):111-129.
[15] BUYER J S,TEASDALE J R,ROBERTS D P,et al.Factors Affecting Soil Microbial Community Structure in Tomato Cropping Systems[J].Soil Biology and Biochemistry,2010,42(5):831-841.
[16] 钟文辉,蔡祖聪,尹力初,等.用PCR2DGGE研究长期施用无机肥对种稻红壤微生物群落多样性的影响[J].生态学报,2007,27(10):4011-4018.[ZHONG Wei-hui,CAI Zu-cong,YIN Li-chu,et al.The Effects of the Long-Term Application of Inorganic Fertilizers on Microbial Community Diversity in Rice-Planting Red Soil as Studied by PCR-DGGE[J].Acta Ecologica Sinica,2007,27(10):4011-4018.]
[17] 夏昕,石坤,黄欠如,等.长期不同施肥条件下红壤性水稻土微生物群落结构的变化[J].土壤学报,2015,52(3):697-705.[XIA Xin,SHI Kun,HUANG Qian-ru,et al.The Changes of Microbial Community Structure in Red Paddy Soil Under Long-Term Fertilization[J].Acta Pedologica Sinica,2015,52(3):697-705.]
[18] 刘若萱,贺纪正,张丽梅.稻田土壤不同水分条件下硝化/反硝化作用及其功能微生物的变化特征[J].环境科学,2014,35(11):4275-4283.[LIU Ruo-xuan,HE Ji-zheng,ZHANG Li-mei.Response of Nitrification/Denitrification and Their Associated Microbes to Soil Moisture Change in Paddy Soil[J].Environmental Science,2014,35(11):4275-4283.]
[19] DENG H,LI X F,CHENG W D,et al.Resistance and Resilience of Cu-Polluted Soil After Cu Perturbation,Tested by a Wide Range of Soil Microbial Parameters[J].FEMS Microbiology Ecology,2009,70(2):137-148.

PLFA Fingerprint of Red Paddy Soils as Affected by Fertilization Pattern and Groundwater Table

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Wen-qian, FEI Xi-min, WANG Cheng-jun. Factors Influencing Excessive Application of Fertilizer [J]. Journal of Ecology and Rural Environment, 2018, 34(8): 726-732.
[2]	WANG Fei, QIN Fang-jin, WANG Xian-ting, WENG Ying, LIN Hui. Study on Effects of Different Fertilization Regimes on Soil Microbial Community Based on MicroResp^TM Method [J]. Journal of Ecology and Rural Environment, 2018, 34(7): 622-629.
[3]	WANG Wen-lin, LIU Bo, HAN Rui-ming, WANG Ye, LIU Xiao, XU Qiao, LI Wen-jing, TANG Xiao-yan. Review of Researches on Factors Affecting Emission of Ammonia From Agriculture [J]. Journal of Ecology and Rural Environment, 2016, 32(6): 870-878.
[4]	LUO Jia, LIU Li-zhu, WANG Tong, YAN Shao-hua, LU Xin, FAN Ru-qin, ZHANG Zhen-hua. Effect of Combined Application of Chemical Fertilizer With Organic Manure on Cucumber Yield and Soil Microbial Diversity [J]. Journal of Ecology and Rural Environment, 2016, 32(5): 774-779.
[5]	LIU Yan, ZHANG Zhen-hua, BAO Wang-bo, YU Ran, WANG Chang-yong. Effects of Penicillin on Soil Microbial Community Structure [J]. Journal of Ecology and Rural Environment, 2016, 32(2): 309-314.
[6]	LIU Qin-pu. Environmental Risk Analyses of Non-Point Source Pollution From Fertilization in Anhui Province, China. [J]. Journal of Ecology and Rural Environment, 2015, 31(6): 876-881.
[7]	YANG Gu-ili, MA Kun, LU Fei, WEI Chang-hui, DAI Xiao-hua . Effect of Continuous Cropping of Potato onAllelo chemicals and soil microbial community. [J]. Journal of Ecology and Rural Environment, 2015, 31(5): 711-717.
[8]	CHEN Lin, QIAO Zhi-Gang, LI Lian-Qing, PAN Gen-Xing. Effects of Biochar-Based Fertilizers on Rice Yield and Nitrogen Use Efficiency [J]. Journal of Ecology and Rural Environment, 2013, 29(5): 671-675.
[9]	XU Ling-Ling, LEI Yun, WANG Zheng-Xiang. Seed Germination and Seedling Establishment of Pterocarya stenoptera as Affected by Groundwater Table and Seeding Depth [J]. Journal of Ecology and Rural Environment, 2013, 29(1): 49-52.
[10]	ZOU Wen-Xiu, HAN Xiao-Zeng, WANG Shou-Yu, JIANG Heng, YANG Chun-Bao. Water consumption by Maize Under Different Fertilization Managements in Black Soil Zone of Northeast China [J]. Journal of Ecology and Rural Environment, 2012, 28(6): 681-686.
[11]	BAI Yuan, XU Hai-Liang, ZHANG Peng, ZHAO Xin-Feng, FU Jin-Yi. Species Diversity and Structural Characters of Desert Plant Communities in Lower Reaches of Tarim River [J]. Journal of Ecology and Rural Environment, 2012, 28(5): 486-492.
[12]	HUANG Jing, ZHANG Yang-Zhu, LIU Hong-Bin, WANG Bo-Ren. CO₂ and N₂O Emissions From Red Soil During Wheat and Corn Growing Seasons Under Different Ptterns of Long-Term Fertilization [J]. Journal of Ecology and Rural Environment, 2011, 27(4): 7-13.
[13]	WANG Xin, WANG Li, ZHAO Feng, CHEN Wei-Ping, YU Xi-Chu, HUANG Qian-Ru, ZHANG Wei-Jian. Effects of Different Long-Term Fertilization Regimes on Crop Productivity and Stress Resistance of the Double Rice Cropping System in the Hilly Area South to the Yangtze River [J]. Journal of Ecology and Rural Environment, 2011, 27(4): 62-68.
[14]	SHI Sheng-Wei, LI Yu-E, QIN Xiao-Bo, WAN Yun-Fan, JI Xiong-Hui. CH₄ Emission From Late Rice Field of Red Clay Soil Under Different Fertilization Treatments [J]. Journal of Ecology and Rural Environment, 2010, 26(2): 103-108.
[15]	HUANG Dong-Feng, WANG Guo, LI Wei-Hua, QIU Xiao-Xuan. Effect of Fertilization Pattern on Yield and Nitrate Content of Vegetables,and Loss of Nitrogen and Phosphorus in Simulated Soil Column [J]. Journal of Ecology and Rural Environment, 2009, 25(2): 68-73.