金花茶遗传多样性和遗传结构AFLP分析

doi:10.19741/j.issn.1673-4831.2018.0236

Abstract

Abstract:

To provide the scientific basis for effective protection and efficient utilization of Camellia nitidissima resource, amplified fragment length polymorphism (AFLP) technology were used to study the genetic diversity and genetic structure of the four natural populations of C. nitidissima. The result show that 1 619 DNA loci were obtained using eight primers, of which, 1 473 loci were polymorphic, and the percentage of polymorphism was 90.98%. The gene diversity and Shannon's information index were 0.146 and 0.246, respectively, at specific level, and that were 0.131 and 0.215 at population level. The change trends of percentage of polymorphic loci, gene diversity and Shannon's information index of four C. nitidissima populations were uniform. The percentage of polymorphic loci ranged from 56.83% to 72.11%. The gene diversity index ranged from 0.122 to 0.138, and the Shannon's information index ranged from 0.197 to 0.228. The genetic differentiation coefficient of C. nitidissima populations was between 0.139 and 0.289, that shows the genetic variation of C. nitidissima was mainly within the populations. The analysis of molecular variance (AMOVA) showed 81.52% of the genetic variation was within the populations, and 18.48% of that was among the populations. The genetic variation of C. nitidissima was mainly within the populations. The unweighted pair group method with arithmetic mean (UPGMA) indicates that there was no significant correlation between the genetic variation of C. nitidissima populations and the geographic distance. Conservation strategies including in situ preservation for promoting natural populations regeneration and ex situ preservation of populations have been put forward.

Key words: Camellia nitidissima, genetic diversity, genetic structure, AFLP, germplasm conservation

CLC Number:

X176

LI Xin-lei, WANG Jie, YIN Heng-fu, FAN Zheng-qi, LI Ji-yuan. Analysis on Genetic Diversity and Genetic Structure of Camellia nitidissima by AFLP Technology[J]. Journal of Ecology and Rural Environment, 2019, 35(1): 63-68.

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Analysis on Genetic Diversity and Genetic Structure of Camellia nitidissima by AFLP Technology

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