Journal of Ecology and Rural Environment ›› 2019, Vol. 35 ›› Issue (9): 1163-1171.doi: 10.19741/j.issn.1673-4831.2018.0638

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Effects of Biochar Addition on Soil Microbial Community Structure Under Different Water Conditions

YU Xiao-yan1, YANG Yan-fang2, ZHANG Ping-jiu1, ZHANG Qun1, DU Yong-gu1   

  1. 1. College of Geography and Tourism, Anhui Normal University/Key Laboratory of Earth Surface Processes and Regional Response in the Yangtze-Huaihe River Basin, Anhui Province, Wuhu 241003, China;
    2. College of Environmental Science and Engineering, Anhui Normal University, Wuhu 241003, China
  • Received:2018-10-12 Published:2019-09-25

Abstract: Soils with biochar pyrolyzed from reed straw at 350 and 600℃, were incubated under different water conditions[75% field water holding capacity (FWHC); drying rewetting cycle; water flooding] for 240 and 720 days, respectively. Soil PLFAs were measured to investigate the effect of water conditions on soil microbial community structure under biochar addition. The results show that biochar addition significantly increased total PLFAs content and the individual group microbial PLFAs content after 240-day incubation under 75% FWHC. However, their PLFAs content was significantly decreased under biochar addition under drying-rewetting cycle and water flooding, especially in the drying-wetting cycle treatment. Moreover, after 240-day incubation, PLFAs content of most microbial groups was higher in soils with biochars at 350℃ than those in soils with biochars at 600℃. Except for actinobacteria under 75% FWHC, comparing with the controls (no biochar addition), total PLFAs content and most group microbial PLFAs content was higher under 240-day incubation than those under 720-day incubation. Different from the controls, except for G+ bacteria, the other groups of microbial PLFAs content in soils with biochar was the highest under water flooding, and the lowest under drying-rewetting cycle. Under 75% FWHC, biochar addition increased soil microbial Shannon-Wiener index (H) and Pielou index (J), and decreased soil Simpson index (D). However, biochar addition decreased soil microbial H and J, and increased D under water flooding only after 240-day incubation. By contrast, biochar addition did not show clear effect on these indexes under drying-rewetting cycle. Redundancy analysis and partial correlation analysis indicated that there was a significant correlation between soil pH, nitrate N, available P and microbial community structures, and more similar soil microbial community structures under the same wet conditions. Moreover, the duration of incubation and water condition controlled the effect of biochar addition on soil microbial community structures. Soil microbial PLFAs content was promoted only by biochar addition under 75% FWHC within 240-day incubation, and decreased or not affected on microbial PLFAs under other treatments.

Key words: water condition, biochar, phospholipid fatty acids (PLFAs), soil microbe, microbial community structure

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