云南滇池海埂水域产毒蓝藻产毒基因的多样性研究

doi:10.19741/j.issn.1673-4831.2018.0688

Abstract

Abstract: Cyanobacteria bloom is a global environmental issue, and the cyanotoxins produced by cyanobacteria seriously endanger aquatic ecosystem and human health. In order to analyze the genetic diversity of cyanotoxins synthesis genes of the toxin-producing cyanobacteria in Dianchi Lake, the expression and sequences of microcystin synthase gene mcyE, anatoxin synthase gene anaC and paralytic shellfish toxins sxtA gene were investigated by Polymerase Chain Reaction(PCR) and TA-cloning-sequencing in Haigeng from March 2017 to February 2018. These three genes were detected in all sampling area. Phylogenetic trees were conducted for analysing of these genes and it was found that the genetic diversity of the three cyanotoxins synthesis genes was relatively single, the mcyE gene was mainly produced by Microcystis, whereas anaC and sxtA genes were mainly produced by Aphanizomenon.

Key words: Dianchi Lake, toxin-producing cyanobacteria, mcyE, anaC, sxtA

CLC Number:

LIU Yu-shan, WANG Yang, LI Chun-xiao, LI Ning-hao, DONG Shu-wei, LIU Li. Genetic Diversity of Cyanotoxins Synthesis Gene of Toxin-producing Cyanobacteria in Haigeng of Dianchi Lake in Yunnan[J]. Journal of Ecology and Rural Environment, 2019, 35(9): 1219-1224.

References

[1] MACÁRIO I P E,CASTRO B B,NUNES I M S,et al.Stepwise Strategy for Monitoring Toxic Cyanobacterial Blooms in Lentic Water Bodies[J].Environmental Monitoring and Assessment,2017,189(12):620.
[2] TRINCHET I,CADEL-SIX S,DJEDIAT C,et al.Toxicity of Harmful Cyanobacterial Blooms to Bream and Roach[J].Toxicon,2013,71:121-127.
[3] LIN H,LIU W Y,ZENG H,et al.Determination of Environmental Exposure to Microcystin and Aflatoxin as a Risk for Renal Function Based on 5493 Rural People in Southwest China[J].Environmental Science & Technology,2016,50(10):5346-5356.
[4] FUNARI E,TESTAI E.Human Health Risk Assessment Related to Cyanotoxins Exposure[J].Critical Reviews in Toxicology,2008,38(2):97-125.
[5] KURMAYER R,CHRISTIANSEN G.The Genetic Basis of Toxin Production in Cyanobacteria[J].Freshwater Reviews,2009,2(1):31-50.
[6] 兰文升,张锐,贺秀媛,等.麻痹性贝类毒素研究进展[C]//2013中国环境科学学会学术年会论文集(第八卷).昆明:2013中国环境科学学会学术年会,2013:6989-6996.
[7] TILLETT D,DITTMANN E,ERHARD M,et al.Structural Organization of Microcystin Biosynthesis in Microcystis aeruginosa PCC7806:An Integrated Peptide-Polyketide Synthetase System[J].Chemistry & Biology,2000,7(10):753-764.
[8] MÉJEAN A,MANN S,MALDINEY T,et al.Evidence that Biosynthesis of the Neurotoxic Alkaloids Anatoxin-a and Homoanatoxin-a in the Cyanobacterium Oscillatoria PCC6506 Occurs on a Modular Polyketide Synthase Initiated by L-proline[J].Journal of the American Chemical Society,2009,131(22):7512-7513.
[9] KELLMANN R,MIHALI T K,JEON Y J,et al.Biosynthetic Intermediate Analysis and Functional Homology Reveal a Saxitoxin Gene Cluster in Cyanobacteria[J].Applied and Environmental Microbiology,2008,74(13):4044-4053.
[10] AL-TEBRINEH J,PEARSON L A,YASAR S A,et al.A Multiplex qPCR Targeting Hepato-and Neurotoxigenic Cyanobacteria of Global Significance[J].Harmful Algae,2012,15:19-25.
[11] VALÉRIO E,CHAMBEL L,PAULINO S,et al.Multiplex PCR for Detection of Microcystins-Producing Cyanobacteria From Freshwater Samples[J].Environmental Toxicology,2009,25(3):251-260.
[12] TILLETT D,PARKER D L,NEILAN B A.Detection of Toxigenicity by a Probe for the Microcystin Synthetase A Gene (mcyA) of the Cyanobacterial Genus Microcystis:Comparison of Toxicities With 16S rRNA and Phycocyanin Operon (Phycocyanin Intergenic Spacer) Phylogenies[J].Applied and Environmental Microbiology,2001,67(6):2810-2818.
[13] LEGRAND B,LESOBRE J,COLOMBET J,et al.Molecular Tools to Detect Anatoxin-a Genes in Aquatic Ecosystems:Toward a New Nested PCR-Based Method[J].Harmful Algae,2016,58:16-22.
[14] BALLOT A,FASTNER J,WIEDNER C.Paralytic Shellfish Poisoning Toxin-Producing Cyanobacterium Aphanizomenon gracile in Northeast Germany[J].Applied and Environmental Microbiology,2010,76(4):1173-1180.
[15] 方红云.浅析滇池蓝藻暴发原因[J].环境科学导刊,2010,29(S1):74-75.[FANG Hong-yun.Brief Talk on Reasons of Blue Algae Bloom in Dianchi Lake[J].Environmental Science Survey,2010,29(S1):74-75.]
[16] 郑丙辉,彭嘉玉,胡小贞,等.2017年滇池外海水质异常下降原因及对策[J].环境工程技术学报,2018,8(5):465-472.[ZHENG Bing-hui,PENG Jia-yu,HU Xiao-zhen,et al.The Cause for Abnormal Water Quality Deterioration of Waihai of Dianchi Lake in 2017 and the Countermeasures[J].Journal of Environmental Engineering Technology,2018,8(5):465-472.]
[17] RANTALA A,FEWER D P,HISBERGUES M,et al.Phylogenetic Evidence for the Early Evolution of Microcystin Synthesis[J].Proceedings of the National Academy of Sciences,2004,101(2):568-573.
[18] RANTALA-YLINEN A,KÄNÄ S,WANG H,et al.Anatoxin-a Synthetase Gene Cluster of the Cyanobacterium Anabaena sp.Strain 37 and Molecular Methods to Detect Potential Producers[J].Applied and Environmental Microbiology,2011,77(20):7271-7278.
[19] 吴忠兴,虞功亮,施军琼,等.我国淡水水华蓝藻-束丝藻属新记录种[J].水生生物学报,2009,33(6):1140-1144.[WU Zhong-xing,YU Gong-liang,SHI Jun-qiong,et al.The Two Newly Recorded Species of Bloom-Forming Cyanobacterial Genus Aphanizomenon Morr.ex Born.et Flah.(C)yanophyta From China[J].Acta Hydrobiologica Sinica,2009,33(6):1140-1144.]
[20] 鲍忠祥.滇池浮游植物和微囊藻毒素的时空分布及细菌去除毒素研究[D].昆明:云南大学,2012.[BAO Zhong-xiang.The Temporal and Spatial Variation of Aquatic Phytoplankton and Microcystin in Dianchi Lake and the Removal of Microcystin With Bacteria[D].Kunming:Yunnan University,2012.]
[21] 万能,宋立荣,王若南,等.滇池藻类生物量时空分布及其影响因子[J].水生生物学报,2008,32(2):184-188.[WAN Neng,SONG Li-rong,WANG Ruo-nan,et al.The Spatio-Temporal Distribution of Algal Biomass in Dianchi Lake and Its Impact Factors[J].Acta Hydrobiologica Sinica,2008,32(2):184-188.]
[22] 张维昊,方涛,徐小清.滇池水华蓝藻中藻毒素光降解的研究[J].中国环境科学,2001,21(1):1-3.[ZHANG Wei-hao,FANG Tao,XU Xiao-qing.Study on Photodegradation of Cyanobacterial Toxin in Blooms of Dianchi Lake[J].China Environmental Science,2001,21(1):1-3.]
[23] 潘晓洁,常锋毅,沈银武,等.滇池水体中微囊藻毒素含量变化与环境因子的相关性研究[J].湖泊科学,2006,18(6):572-578.[PAN Xiao-jie,CHANG Feng-yi,SHEN Yin-wu,et al.The Correlation Between the Variation of Microcystin Content and Environment Factors in Dianchi Lake[J].Journal of Lake Sciences,2006,18(6):572-578.]
[24] TSUJI K,SETSUDA S,WATANUKI T,et al.Microcystin Levels During 1992-1995 for Lakes Sagami and Tsukui-Japan[J].Natural Toxins,1996,4(4):189-194.
[25] 刘永梅,刘永定,李敦海,等.滇池束丝藻水华毒性生物检测[J].水生生物学报,2004,28(2):216-218.[LIU Yong-mei,LIU Yong-ding,LI Dun-hai,et al.Bioassay for the Toxicity of Aphanizomenon Flos-Aquae Bloom From Lake Dianchi[J].Acta Hydrobiologica Sinica,2004,28(2):216-218.]

Genetic Diversity of Cyanotoxins Synthesis Gene of Toxin-producing Cyanobacteria in Haigeng of Dianchi Lake in Yunnan

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Recommended Articles

Metrics

Comments

[1]	JI Bo-hua, LI Wei, CHANG Jun-jun, LI Xuan, SI Guang-zheng, HE Fang, CHEN Jin-quan. Characteristics of Bacterial Community Structure in Sediments With Different Emergent Plants in Dianchi Lakeside Wetland [J]. Journal of Ecology and Rural Environment, 2020, 36(3): 390-398.
[2]	ZHANG Ying-Ying, ZHANG Zhi-Yong, WANG Ya-Lei, LIU Hai-Qin, WANG Zhi, YAN Shao-Hua, HAN Ya-Ping, YANG Lian. Research on the growth characteristics and accumulation ability to N and P of water hyacinth in different water area of Dianchi Lake [J]. Journal of Ecology and Rural Environment, 2011, 27(6): 73-77.