Journal of Ecology and Rural Environment ›› 2019, Vol. 35 ›› Issue (7): 892-899.doi: 10.19741/j.issn.1673-4831.2018.0692

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Colonization and Growth Promotion of Three Bacillus Strains in Rice Rhizosphere and Their Survival in Soil

WANG Heng-xu1,2, LIU Ze-ping1,2, WANG Zhi-gang1,2, XU Wei-hui1,2, GUO Ru-xin1,2   

  1. 1. Heilongjiang Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas, Qiqihar University, Qiqihar 161006, China;
    2. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China
  • Received:2018-10-30 Online:2019-07-25 Published:2019-07-22

Abstract:

Plant growth-promoting rhizobacteria (PGPR) can secrete plant growth hormone and promote soil nutrient cir-culation,which is an important germplasm resource of biological fertilizers. Adsorption of PGPR to crop roots is a neces-sary condition for colonization and function,and drug resistance marker is a mean of studying the ability of functional strains to colonize and promote growth. The purpose of the experiment is to label the strain by the antibiotic drug rifampi-cin,and to study the retention of the function of the strain,and then to determine the colonization survival ability of the strain in the rhizosphere of rice. The results show that the adsorption of rice roots to strain increased with the immersion time,and the adsorption content of K-LZP01 was basically stable after dipping for 10 min. K-LZP02 reached the maximum adsorption capacity at 50 min. The maximum adsorption capacity of K-LZP03 was 12×107 CFU·g-1 at 5 min,and then be-came stable. When the rice seedlings were dipped in different concentrations of bacterial suspension, the adsorption capac-ity of radicle increased with increasing of bacterial suspension concentration. After 10 days of inoculation in soil,the colo-nization quantity of the labeled strains tended to be stable and the colonization ability is strong. The results found that the closer the distance to the rice rhizosphere,the higher the strain content,which indicate that the strains had good coloniza-tion competitiveness. In both sterilized and unsterilized soils,the three strains could all promote the growth of rice. In this study,researches on the resistance marker of PGPR strain as well as its colonization and growth promotion in rice rhizo-sphere were carried out,and the results are important to interpret the mechanism of PGPR and guide the production prac-tice.

Key words: Bacillus, resistance marker, colonization, adsorption, rice

CLC Number: