畜禽养殖场污水处理系统中诺氟沙星抗性菌的筛选及其抗性基因分析

doi:10.19741/j.issn.1673-4831.2019.0343

Abstract

Abstract: The accumulation and diffusion of antibiotic resistant bacteria and resistance genes in wastewater treatment systems of livestock farm poses potential hazards to ecological environment and human health. Therefore, it is necessary to investigate the types of resistant bacteria, resistance genes and genetic characteristics in the livestock wastewater treatment systems. In this study, a norfloxacin-resistant strain TY-1 was isolated from the sludge, which was collected from the secondary sedimentation tank of a meat goose farm in Jiangsu Province. The strain TY-1 was identified as Sphingomonas sp., according to the results of morphological, physiological and biochemical characteristics, and 16S rRNA gene sequence analysis. Based on the whole genome annotation of Sphingomonas sp., it was found that strain TY-1 had multiple antibiotic resistance genes (ARGs), including ABC transporter, MFS transporter and cytochrome P450 dehydrogenase. According to the results of gene cluster analysis, these ARGs were ubiquitous in genus of Sphingomonas. This study can provide a useful reference for the ecological risk assessment of pathogenic microorganisms containing antibiotic resistance genes in the wastewater treatment systems of livestock farm.

Key words: livestock farm, fluoroquinolones, Sphingomonas sp., antibiotic resistance genes

CLC Number:

X172

ZHAO Han-bin, CHEN Xin, WU Guang-zhe, ZHOU Chun-chun, LIU Li-li. Isolation of Norfloxacin Resistant Bacteria and Analysis of Its Antibiotic Resistance Genes From the Wastewater Treatment System of a Livestock Farm[J]. Journal of Ecology and Rural Environment, 2020, 36(2): 250-256.

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Isolation of Norfloxacin Resistant Bacteria and Analysis of Its Antibiotic Resistance Genes From the Wastewater Treatment System of a Livestock Farm

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[1]	ZHAO Jing, BI Chun-juan, CHEN Zhen-lou, SHI Qing, LI Lin-ru, YAO Chun-xia. Contents of Fluoroquinolone-Type Antibiotics in the Surroundings of Livestock Farms in Chongming Island of Shanghai [J]. Journal of Ecology and Rural Environment, 2017, 33(2): 120-126.
[2]	GU Xiao-ming, XING Ke-xia, YI Li-jun, CHEN Yu-qiao, LIU Qing-yang, XU Xiang-bo, GAO Shang-bin, MA Zhong. Introduction of Public-Private-Partnership (PPP) Into Comprehensive Utilization of Livestock Waste From Rural Livestock Keepers [J]. Journal of Ecology and Rural Environment, 2017, 33(1): 62-69.