Journal of Ecology and Rural Environment ›› 2020, Vol. 36 ›› Issue (4): 488-498.doi: 10.19741/j.issn.1673-4831.2019.0731

Previous Articles     Next Articles

Effects of Flooding Stress on Physiological Characteristics and Rhizosphere Bacterial Community of Acorus calamus

GU Shi-yun1,2, YANG Fei2, ZHANG Yi-min2, ZHANG Zhi-wei2, XIE Ke-fu1,2, GUAN Xiang-yang1,2   

  1. 1. College of Environmental and Safety Engineering, Changzhou University, Changzhou 213164, China;
    2. Nanjing Institute of Environmental Science, Ministry of Ecology and Environment, Nanjing 210042, China
  • Received:2019-09-11 Online:2020-04-25 Published:2020-04-27

Abstract: The typical emergent plant Acorus calamus was selected for a in-situ simulation experiment in Gehu Lake. Effects of different treatments on physiological characteristics and rhizosphere bacterial community of Acorus calamus were investigated at different flooding depths (T0, T1, T2 referred to normal water level, half-submerged water level about 40 cm and completely submerged water level about 80 cm, respectively) and flooding duration (within an experimental period of 42 d). The results show that: (1) Acorus calamus could adapt to 28 d half-submerged environment, but more than 35 d half-submerged or more than 21 d completely submerged environment would aggravate leaf membrane lipid peroxidation, and increase the content of malondialdehyde (MDA), significantly inhibiting the activity of superoxide dismutase (SOD) and the photosynthesis of plant leaves (P<0.05). (2) Comparing the characteristics of rhizosphere bacterial community of Acorus calamus at the beginning of flooding (0 d) and the end of flooding (42 d), it was found that flooding led to the change of rhizosphere bacterial community structure of Acorus calamus, which was less similar to that at the beginning of flooding, but the similarity between different flooding depths was higher. The diversity and richness of rhizosphere bacterial community of Acorus calamus were increased and there were more unique bacterial species at the end of flooding. There was no significant difference in the composition of bacterial community at the level of phylum, class, order, family, genus, but the composition quantity was different. At the level of phylum, the abundance of Cyanobacteria and Firmicutes increased and the abundance of Proteobacteria decreased at the end of flooding. At the level of genus, the abundance of aerobic flora such as Pseudomonas, Thiobacillus, Sulfuricurvum decreased, and the abundance of anaerobic flora such as Prevotella increased, which was related to the low oxygen environment in the rhizosphere caused by flooding.

Key words: Acorus calamus, flooding stress, physiological characteristics, rhizosphere bacterial community

CLC Number: