苯并[a]芘降解菌在不同土壤无机胶体表面的吸附研究

doi:10.19741/j.issn.1673-4831.2022.0061

Abstract

Abstract: Based on isothermal adsorption experiments, Ex-DLVO theory, Fourier transform infrared spectroscopy (FTIR) and other means were used to explore the interface interaction mechanism between typical soil colloids (kaolinite, montmorillonite and hematite) and Paracoccus aminovorans HPD-2, which is a highly efficient degrading bacteria of benzo[a]pyrene. The results show that the isotherm adsorption curves of the three typical soil inorganic colloids on HPD-2 cells were all in accordance with Langmuir adsorption model, and the theoretical maximum adsorption capacity was hematite >> montmorillonite > kaolinite. The Ex-DLVO theory results show that the adsorption process of hematite on HPD-2 cells was spontaneous, while the adsorption of kaolinite and montmorillonite on degrading bacteria needed to cross the energy barrier. The electrostatic force between colloidal particles and cells played a key role in the adsorption process, which was much stronger than van der Waals force and hydrophobic force. FTIR results show that the interaction of kaolinite and montmorillonite on HPD-2 was mainly due to the change of protein configuration on cell surface, and the interaction was weak. In addition to hydrogen bond, strong P-OFe bond was formed between hematite and strain HPD-2. The results elucidate the mechanisms of interface interaction between inorganic mineral colloids with different properties and HPD-2 and can provide theoretical basis for the studies on the effect mechanism of inorganic colloids on the degrading microorganisms viability and colonization on their surface.

Key words: soil inorganic colloid, benzo[a]pyrene degrading bacteria, Ex-DLVO theory, interface interaction

CLC Number:

X53
S153.6

GAN Xin-hong, TENG Ying, MU Ting-ting, LIU Guo-qiang, XU Jian, LUO Yong-ming. Adsorption of Benzo[a]pyrene Degrading Bacteria on Different Inorganic Mineral Colloids[J]. Journal of Ecology and Rural Environment, 2023, 39(1): 115-122.

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Adsorption of Benzo[a]pyrene Degrading Bacteria on Different Inorganic Mineral Colloids

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