Determination of Chlordane Residue in Aquatic Products With Gas Chromatography

To establish a method for determination of cis-chlordane,trans-chlordane and oxychlordane in aquatic products with gas chromatography,edible parts of fish,shrimps and shells were used for the test.One granm of homogenized tissues was treated with acetonitrile,extracted ultrasonically,stripped with hexane,and refined through a Florisil silica-gel column.And the outflow from the column was analyzed on a gas chromatograph equipped with electron capture detector for determination of chlordane.The linear range of chlordane concentration was from 0.200 to 100g•L-¹(r=0.998 3～0.999 9，n=7,P<0.001),and the detection limit for the three species of chlordane was all 2.00 ug•kg^-1(in wet weight).When the sample was added at a rate of 1 to 15 times as much as the quota,the recovery rate of the sample ranged from 72.2% to 104.0% based on 6 repeated determinations.Variation coefficient varied from 2.81% to 8.56% in each batch(n=6)and was 8.50% between batches(n=3).