磺胺二甲嘧啶对沼气发酵过程中酶活性和微生物群落功能多样性的影响

doi:10.11934/j.issn.1673-4831.2017.07.010

Abstract

Abstract:

With the development of livestock and poultry breeding industry, veterinary antibiotics are widely used as feed additives in domestic animal farming, either to improve the growth performance or to prevent infection, resulting in an increase in the total amount of livestock fecal contamination as well as antibiotic contamination. In order to investigate the effect of veterinary antibiotics on biogas fermentation, an experiment was designed to study the effects of sulfamethazine (SMZ) addition in the pig manure on enzyme activities and microbial community functional diversity in the biogas fermentation process with pig manure and corn stalks as raw materials. The results show that T1 treatment (20 mg·kg^-1) and T2 treatment (60 mg·kg^-1) would promote the urease activities in the initial stage of biogas fermentation(from day 3 to day 6), since then on, it was decreased. The dehydrogenase activity in T3 treatment (120 mg·kg^-1) was inhibited at the beginning of the composting process and then was promoted. The effect of sulfamethazine addition on microbial community functional diversity during biogas fermentation was assayed with Biolog method. The results show that AWCD (average well color development) values in T2 and T3 treatment was inhibited at the beginning of the composting process and then was promoted. Shannon index and Simpson index results suggest that the sulfamethazine addition could significantly reduce the functional diversity of microbial communities in the initial stage of the biogas fermentation (in the first 6 days), in contrast, it could increase the functional diversity of microbial communities in the first 35 days. It is obvious that sulfamethazine has significant effect on hydrolytic enzyme activities and microbial community functional diversity in the pig manure biogas fermentation process. The effect of sulfamethazine on hydrolytic enzyme activities and microbial community functional diversity are also different in the different stages of biogas fermentation.

Key words: sulfamethazine, biogas fermentation, enzyme activity, microbial community

CLC Number:

X705

ZHANG Min, ZHANG Jun, QIAN Jin-qiu, LIU Qing-yu, SHAO Ming-jie, YIN Si-yuan. Effects of Sulfamethazine on Enzyme Activities and Microbial Community Functional Diversity During Biogas Fermentation[J]. Journal of Ecology and Rural Environment, 2017, 33(7): 653-659.

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Effects of Sulfamethazine on Enzyme Activities and Microbial Community Functional Diversity During Biogas Fermentation

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