六氯苯胁迫下2种湿地植物根际土壤微生物数量与酶活性变化

doi:10.11934/j.issn.1673-4831.2018.02.011

Abstract

Abstract:

To investigate plant rhizospheric effects on biodegradation of chlorobenzene compounds in constructed wetlands, Phragmites australis and Typha angustifolia, two typical wetland plants, were selected and planted in a simulated outdoor constructed wetland mesocosm to explore changes in population of soil microbe and activity of soil enzyme in the rhizosphere of the plants under stress of hexachlorobenzene. Results show that both P. australis and T. angustifolia significantly affected soil microbe (bacteria, fungi and actinomycetes) in population and soil enzymes[dehydrogenase (DEH), catalase (CAT) and polyphenol oxidase (PPO)], in activity in the rhizosphere, which were all higher than in the non-rhizosphere soils, and the rhizosphere effects of T. angustifolia were higher than those of P. australis (P<0.05). Population of the soil microbe and activities of the soil enzymes were found negatively related to concentration of HCB in the rhizospheres of P. australis and T. angustifolia,and particularly to the activities of DEH and PPO in the rhizospheres of T. angustifolia(P<0.01). The rhizospheric effects of the two plants increased with the increase in microbial population and enzyme activity in the rhizospheres of the plants, which in turn helps remove HCB in the wetland ecosystem.

Key words: constructed wetland, rhizospheric effect, microbial population, enzyme activity, hexachlorobenzene

CLC Number:

X171.5

ZHANG Cui-ping, LI Shu-ying, WANG Bei, LU Guo-li, ZHU Chun-rong, ZHOU Yuan-qing. Changes in Population of Soil Microbe and Activity of Soil Enzyme in Rhizospheres of Two Wetland Plants Under Stress of Hexachlorobenzene[J]. Journal of Ecology and Rural Environment, 2018, 34(2): 177-183.

References

[1] ZHOU X,ZHANG C F,ZHANG D D,et al.Polyphasic Characterization of an Anaerobic Hexachlorobenzene Dechlorinating Microbial Consortium With a Wide Dechlorination Spectrum for Chlorobenzenes[J].Journal of Bioscience and Bioengineering,2015,120(1):62-68.
[2] SPECHT I O,BONDE J P E,TOFT G,et al.Environmental Hexachlorobenzene Exposure and Human Male Reproductive Function[J].Reproductive Toxicology,2015,58:8-14.
[3] 刘辉,姜林,王琪,等.六氯苯微生物降解研究进展[J].环境污染与防治,2013,35(1):86-92.[LIU Hui,JIANG Lin,WANG Qi,et al.Progress on Microbial Degradation of Hexachlorobenzene[J].Environmental Pollution and Control,2013,35(1):86-92.
[4] GUO W,ZHANG H Y,HUO S L.Organochlorine Pesticides in Aquatic Hydrophyte Tissues and Surrounding Sediments in Baiyangdian Wetland,China[J].Ecological Engineering,2014,67:150-155.
[5] PERELLÓ G,GÓMEZ-CATALÁN J,CASTELL V,et al.Estimation of the Daily Intake of Hexachlorobenzene From Food Consumption by the Population of Catalonia,Spain:Health Risks[J].Food Control,2012,23(1):198-202.
[6] BASTERRECHEA M,LERTXUNDI A,IÑIGUEZ C,et al.Prenatal Exposure to Hexachlorobenzene (HCB) and Reproductive Effects in a Multicentre Birth Cohort in Spain[J].Science of the Total Environment,2014,466/467:770-776.
[7] YAN D Z,MAO L Q,LI C Z,et al.Biodegradation of Hexachlorobenzene by a Constructed Microbial Consortium[J].World Journal of Microbiology and Biotechnology,2015,31(2):371-377.
[8] UPADHYAY A K,BANKOTI N S,RAI U N.Studies on Sustainability of Simulated Constructed Wetland System for Treatment of Urban Waste:Design and Operation[J].Journal of Environmental Management,2016,169:285-292.
[9] WU J,FENG Y Q,DAI Y R,et al.Biological Mechanisms Associated With Triazophos (TAP) Removal by Horizontal Subsurface Flow Constructed Wetlands (HSFCW)[J].Science of the Total Environment,2016,553:13-19.
[10] ZHOU Y Q,TIGANE T,LI X Z,et al.Hexachlorobenzene Dechlorination in Constructed Wetland Mesocosms[J].Water Research,2013,47(1):102-110.
[11] ZHOU Y Q,TRESTIP S,LI X Z,et al.Dechlorination of Hexachlorobenzene in Treatment Microcosm Wetlands[J].Ecological Engineering,2012,42:249-255.
[12] TORO-VÉLEZ A F,MADERA-PARRA C A,PEÑA-VARÓN M R,et al.BPA and NP Removal From Municipal Wastewater by Tropical Horizontal Subsurface Constructed Wetlands[J].The Science of the Total Environment,2016,542(Pt A):93-101.
[13] STANISLAW B,BIELINSKA E J,PATRYK O.Enzymatic Activity in an Airfield Soil Polluted With Polycyclie Aromatic Hydrocarbons[J].Geoderma,2004,118(3/4):221-232.
[14] ZHAO X Y,YANG J X,BAI S W,et al.Microbial Population Dynamics in Response to Bioaugmentation in a Constructed Wetland System Under 10℃[J].Bioresource Technology,2016,205:166-173.
[15] WANG M C,LIU Y H,WANG Q,et al.Impacts of Methamidophos on the Biochemical,Catabolic,and Genetic Characteristics of Soil Microbial Communities[J].Soil Biology and Biochemistry,2008,40(3):778-788.
[16] LIU C Y,JIANG X,WANG F,et al.Hexachlorobenzene Dechlorination as Affected by Nitrogen Application in Acidic Paddy Soil[J].Journal of Hazardous Materials,2010,179(1/2/3):709-714.
[17] 郭强,田慧,吴有方,等.甘肃省及其周边地区土壤六氯苯污染特征[J].中国环境科学,2013,33(3):455-460.[GUO Qiang,TAIN Hui,WU You-fang,et al.Soil Pollution Characteristics of Hexachlorobenzene in Gansu Province and Its Neighboring Regions[J].China Environmental Science,2013,33(3):455-460.]
[18] 鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,2000:25-151.[BAO Shi-dan.Soil and Agricultural Chemical Analysis[M].3rd ed.Beijing:China Agriculture Press,2000:25-151.]
[19] 李振高,骆永明,腾应.土壤与环境微生物研究法[M].北京:科学出版社,2008:395-399.[LI Zhen-gao,LUO Yong-ming,TENG Ying.Research Methods of Soil Environmental Microorganism[M].Beijing:Science Press,2008:395-399.]
[20] TOYAMA T,FURUKAWA T,MAEDA N,et al.Accelerated Biodegradation of Pyrene and Benzo[a]Pyrene in the Phragmites australis Rhizosphere by Bacteria-Root Exudate Interactions[J].Water Research,2011,45(4):1629-1638.
[21] 孙约兵,王润珑,徐应明,等.除草剂硝磺草酮对土壤微生物生态效应研究[J].中国环境科学,2016,36(1):190-196.[SUN Yue-bing,WANG Run-long,XU Ying-ming,et al.Ecological Effects of Herbicide Mesotrione on Soil Microbial Communities[J].China Environmental Science,2016,36(1):190-196.]
[22] 刘亚云,陈桂珠.植物修复多氯联苯研究进展[J].应用生态学报,2006,17(2):325-330.[LIU Ya-yun,CHEN Gui-zhu.Research Advances in Phytoremediation of Polychlorinated Biphenyls (PCBs)[J].Journal of Applied Ecology,2006,17(2):325-330.]
[23] MA X M,HAVELKA M M.Phytotoxicity of Chlorinated Benzenes to Typha angustifolia and Phragmites communis[J].Environmental Toxicology,2009,24(1):43-48.
[24] 苏嘉欣,卓胜,黎华寿,等.植物间作下根际微生物和土壤酶与土壤多氯联苯(PCB)降解的关系[J].农业环境科学学报,2010,29(11):2114-2120.[SU Jia-xin,ZHUO Sheng,LI Hua-shou,et al.Effects of Rhizospheric Microbes and Soil Enzymes on the Biodegradation of PCB in the Soil Within Intercropping Systems[J].Journal of Agro-Environment Science,2010,29(11):2114-2120.]
[25] 钱鸣飞,李勇,黄勇.芦苇和香蒲人工湿地系统净化微污染河水效果比较[J].工业用水与废水,2008,39(6):55-58.[QIAN Ming-fei,LI Yong,HUANG Yong.Comparison of Treatment Effects of Micro-Pulluted River Water by Constructed Wetlands Planted With Phragmites communis and Typha latifolia[J].Industrial Water & Wastewater,2008,39(6):55-58.]
[26] BENDING G D,TURNER M K,RAYNS F,et al.Microbial and Biochemical Soil Quality Indicators and Their Potential for Differentiating Areas Under Contrasting Agricultural Management Regimes[J].Soil Biology & Biochemistry,2004,36(11):1785-1792.
[27] ZORNOZA R,GUERRERO C,MATAIX-SOLERA J,et al.Assessing Air Drying and Rewetting Pre-Treatment Effect on Some Soil Enzyme Activities Under Mediterranean Conditions[J].Soil Biology & Biochemistry,2006,38(8):2125-2134.
[28] IYYEMPERUMAL K,SHI W.Soil Enzyme Activities in Two Forage Systems Following Application of Different Rates of Swine Lagoon Effluent or Ammonium Nitrate[J].Applied Soil Ecology,2008,38(2):128-136.
[29] ZHANG C P,WANG B,DAI X Y,et al.Structure and Function of the Bacterial Communities During Rhizoremediation of Hexachlorobenzene in Constructed Wetlands[J].Environmental Science and Pollution Research,2017,24(12):11483-11492.
[30] FAULWETTER J L,BURR M D,PARKER A E,et al.Influence of Season and Plant Species on the Abundance and Diversity of Sulfate Reducing Bacteria and Ammonia Oxidizing Bacteria in Constructed Wetland Microcosms[J].Microbial Ecology,2013,65(1):111-127.
[31] ZHANG T,XU D,HE F,et al.Application of Constructed Wetland for Water Pollution Control in China During 1990-2010[J].Ecological Engineering,2012,47:189-197.
[32] 何起利,梁威,贺锋,等.人工湿地氧化还原特征及其与微生物活性相关性[J].华中农业大学学报,2007,26(6):844-849.[HE Qi-li,LIANG Wei,HE Feng,et al.Correlations Between the Redox Potential Characteristics and Microorganisms Activities in the Integrated Vertical Flow Constructed Wetland[J].Journal of Huazhong Agricultural University,2007,26(6):844-849.]
[33] GERMAN D P,WEINTRAUB M N,GRANDY A S,et al.Optimization of Hydrolytic and Oxidative Enzyme Methods for Ecosystem Studies[J].Soil Biology & Biochemistry,2011,43(7):1387-1397.

Changes in Population of Soil Microbe and Activity of Soil Enzyme in Rhizospheres of Two Wetland Plants Under Stress of Hexachlorobenzene

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