Journal of Ecology and Rural Environment ›› 2012, Vol. 28 ›› Issue (5): 587-594.doi:
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GE Feng, GUO Kun, TAN Li-Chao, DAI Yi-Jun
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Abstract: A new method using SPE-UPLC-MS/MS was developed to determine residues of four typical categories of veterinary antibiotics in organic manure. The method uses the solution composed of acetonitrile and phosphate buffer (pH=3) for extraction of organic manure samples, and the series of SAX and HLB to purify and enrich the extract. Methanol-φ=0.1% formic acid was chosen as carrier for liquid chromatographic analysis. When the concentrations of the 18 antibiotics were in the range of 5-500µg•kg-1, they showed good linear relationships, with determination coefficients (r2) ≥ 0.99. When the mass concentration of the antibiotics was raised to the range of 50-500µg•kg-1, the recovery rates of sulfonamides, fluoroquinolones, tetracyclines and macrolides varied in the range from 71.6% to 94.3%, from 72.3% to 86.7%, from 71.0% to 102.2% and from 70.7% to 93.3%, respectively, with relative standard deviation varying in the range of from 2.1% to 11.4%. This method was applied to determine residues of veterinary antibiotics in organic fertilizers on sale in Nanjing. In addition to sulfonamides, others were also detected to a varying extent.
Key words: SPE-UPLC-MS/MS, organic fertilizers, veterinary drug residue
CLC Number:
X830
O657.63
GE Feng, GUO Kun, TAN Li-Chao, DAI Yi-Jun. Simultaneous Determination of Residues of Four Typical Categories of Veterinary Antibiotics in Organic Fertilizers[J]. Journal of Ecology and Rural Environment, 2012, 28(5): 587-594.
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