Effects of F- and Cl-on Growth of Chlamydomonas reinhardtii
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Graphical Abstract
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Abstract
To explore toxic effects of F- and Cl- on growth of Chlamydomonas reinhardtii for comparison, algal biomass, specific growth rate and growth inhibition rate as affected by concentration of F- or Cl- were determined with the Erlenmeyer flask cultivation method and model analysis. Results show that F- in the range of 5-80 mg·L-1 was toxic to C. reinhardtii, inhibiting its growth and the growth inhibition rate increased with increasing exposure time and F- concentration, but its maximum growth inhibition rate dropped below 62% on D 10, which suggests that C. reinhardtii is quite tolerable to F- below 80 mg·L-1 in concentration. By contrast, Cl- could either promote or inhibit growth C. reinhardtii of depending on Cl- concentration. When Cl- concentration was up to 12800 mg·L-1, its growth inhibition rate reached as high as 96%. F- and Cl- differs in effect on growth of C. reinhardtii, which is reflected mainly in the laws of the functions of the growth effect models varying with F- or Cl- concentration. Besides, in terms of EC50 under the same exposure time F- (18.0 to 56.4 mg·L-1) was far too lower than Cl- (1 074 to 2 360 mg·L-1). That is to say, F- is more toxic to C. reinhardtii than Cl-,but the ecological risk of Cl- in the environment may not be ignored, either.
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