Journal of Ecology and Rural Environment ›› 2020, Vol. 36 ›› Issue (3): 399-405.doi: 10.19741/j.issn.1673-4831.2019.0625

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Effects of Cr6+ Exposure on Oxidative Stress and Reproductive Gene Expression in the Ovaries of Polymesoda erosa

CHENG Sui1,2,3, LU Feng1,2,3, HUANG Bo1,2,3, GUO Yun-peng1,2,3   

  1. 1. Ocean College of Hainan University, Haikou 570228, China;
    2. State Key Laboratory of Marine Resource Utilization in South China Sea, Haikou 570228, China;
    3. Key Laboratory of Tropical Biological Resources of Ministry of Education, Haikou 570228, China
  • Received:2019-08-12 Online:2020-03-25 Published:2020-03-25

Abstract: Cr6+ can usually cause lipid peroxidation stress in organisms due to its strong oxidative capacity. In this study, the reproductive toxicity of Cr6+ exposure on female Polymesoda erosa was investigated. Five concentration groups of Cr6+ were set as 0, 0.25, 1, 4 and 8 mg·L-1 and the exposure time of each concentration group was set as 0, 5, 10 and 15 d. The results indicate that the exposure to Cr6+ could cause oxidative stress on the ovaries of Polymesoda erosa and change the expression of er (estrogen receptor gene) and vtg (vitellogenin gene). Compared with control group, the high concentration of Cr6+ treatment groups could significantly reduce the gonadosomatic index (GSI) of Polymesoda erosa (P<0.05). Moreover, the GSI varied significantly with the increase of Cr6+ concentration as time goes by. The expression of vtg and er in high concentration treatment group was significantly lower than that in low concentration treatment group as well as control group (P<0.05). In addition, it was found that the activities of CAT, GST and MDA content in ovaries would get back to normal level (P>0.05) after exposing to low concentration of Cr6+. On the other hand, high concentration of Cr6+ exposure would eventually inhibit the activities of both enzymes (P<0.05) and increase the content of MDA significantly. The results manifest that Cr6+ has tissue damage and metabolic disturbance effects on the ovaries of Polymesoda erosa and high concentration of Cr6+ would cause obvious toxic effects on the reproduction of Polymesoda erosa.

Key words: Polymesoda erosa, ovary, Cr6+, gonad index, enzyme activity, reproductive toxicity

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