Multi-phase extraction(MPE ) technology is one of the mainstream technologies now applicable in the world for remediation of soil and groundwater polluted by organic pollutants. Typically, it pumps simultaneously soil gas, groundwater and non-aqueous phase liquid up to the ground for separation and treatment so as to achieve rapid pollution control and simultaneous remediation of polluted soil and groundwater. To deepen the knowledge of the technology, process features and technical principles of MPE are introduced, and key points and designing ideas of MPE and its advantages and disadvantages relative to conventional remediation technologies summarized. In light of the characteristics of the MPE technology, methods for evaluation of applicability of the technology in areas with specific soil aeration zone, water-containing layer and pollutants are analyzed and summarized. In the end, several typical cases of application of the MPE technology are cited.

Bio-piracy is an increasingly serious problem for China and many other developing countries rich in biodiversity. Biotechnological companies in developed countries grab genetic resources and associated traditional knowledge illegally from developing countries, and then make excessive profit through bio-prospecting and product patent protection from the markets of the countries providing the genetic resources. From literature, databases and relevant websites, a total of 108 biopiracy cases of the past 30 years were gathered involving agriculture, bio-pharmacy, cosmetics and other industries for analysis in line with the following six aspects, that is, application area,target resourse,user institution,usage of the resource, origin of the resource, benefit-sharing implementation and bio-piracy in China. Furthermore, in view of the biopiracy problem and shortage of legislation and institutional organization for protection of genetic resources in China, it is proposed in this paper to work out national laws and regulations for acquisition of genetic resources and related traditional knowledge and profit sharing, including implementation of “prior informed consent (PIC)” for acquisition of genetic resources and “mutually agreed terms (MAT)” for ensurance of fair and equitable benefit-sharing between providers and users of the resources, and meanwhile, to strengthen capacity-building for governmental organizations in management.  

A field survey of agricultural soils in the Sanjiang Plain was carried out of agricultural soils using the grid sampling method for analysis of spatial distribution of heavy metals (As, Cr, Cu, Pb and Zn) in the soil by using the geo-statistics and traditional statistics methods. The studied area was 7.31× 10⁴ km² in total and it was divided into 16km×16 km grids, providing 244 sampling points. Results show that in this area, the heavy metals varied sharply in terms of spatial distribution in the soil with variation coefficients ranging from 30.67%-66.89%; The heavy metals were all quite high in spatial autocorrelation and their spatial autocorrelation was rather wide in range. As and Cr was <20% in C₀/(C₀+C), showing their spatial variations were mainly affected by natural factors, like soil parent material, topography and climate, while Cu, Pb and Zn varied in the range of 35.8%-43.2% in C₀/(C₀+C),  showing that their main affecting factors were non-structural ones; The heavy metals differed in spatial distribution and were quite obvious in spatial variation on a small area scale. They did not have any specific patterns in spatial distribution on a large area scale, except Cu. A surface soil heavy metals spatial distribution map was worked out using the Kriging interpolation method, revealing ,that heavy metal concentrations in the soil are closely related to soil type, physical-chemical properties, land-use pattern, mining, agricultural mechanization intensity, and agricultural irrigation.  

Production of animal manure and pollutants therein, such as chemical oxygen demand (COD_Cr), ammonia nitrogen (NH₃-N), total nitrogen (TN) and total phosphorus (TP), were first estimated using the parametric estimating method based on the numbers of various livestock in the Poyang Lake ecological economic zone in the year of 2011, and then load of animal mature on cropland in each county (city or district) was figured out based on the area of cropland in the county, and in the end, animal matnure load tolerance degree of each county was evaluated in light of the alarming value of animal manture load on cropland. Results show that in 2011, the zone turned out a total of 31 294 200 tons of animal manure, which contained 833 400 tons of COD_Cr, 69 700 tons of NH₃-N, 171 900 tons of TN and 57 900 tons of TP, accounting for about 40% of the total of the province. The analysis in light of the alarming value of animal manture load on cropland indicates that in the Poyang Lake ecological economic zone, 9 counties (cities, districts) were still below the level of animal manure load posing a potential threat to the local environment, while the other 26 counties (cities, districts) theoretically were above or at the level, especially, Qingyunpu district of Nanchang and Xunyang district of Jiujiang.

Based on the data of the first national survey of pollution, production, discharge and river pollution load of various agricultural pollutants (COD, TN and TP) wereas calculated using their respective production and discharge coefficients. Comparative analysis was done of the status quos of crop cultivation, livestock and poultry rearing and aquaculture in pollutant discharge. Results show that the three sectors differed sharply in proportion of COD, TN, and TP to the total pollutant discharged. COD and TN accounted for 52% and 45%, respectively, of the total pollutant discharged from crop cultivation; and COD accounted for 91% of the total pollutant discharged from livestock rearing and for 80% of the total pollutant discharged from aquaculture. The three sectors also differed sharply in contribution to the total COD, TN and TP discharges from the agriculture in the region. Aquaculture and livestock and poultry breeding were the main contributors of COD to the total from agriculture, accounting for more than 80%; crop cultivation contributed 60% of the TN discharged from agriculture and aquaculture did nearly 60% of the TP discharged from agriculture. Therefore, to reduce organic pollutants, it is essential to begin with controlling measures in livestock and poultry breeding and aquaculture; to reduce nitrogen-like pollutants, in crop cultivation; and to reduce phosphorus-like pollutants, in aquaculture. The findings may provide some valuable references for reducing agricultural pollution and designing pollution controlling measures.

A case study was done of Daxing District in Beijing, using the landscape pattern and spatial analysis method, to explore characteristics of spatial variation of land use in metropolitan suburban areas from the angle of distribution and location of different industries. Results show that 1) the district as a whole is high in land use rate, but not even. The northern part is higher than the southern part in comprehensive level of land use as a result of distribution of different industries and their locations varying in distance from the city, towns, villages and roads, and  key towns are higher than general towns in complehensive level of land use; 2) agricultural lands high in intensity of land use, such as facilitated farmland, orchard land and facilitated arable land, account for a large percentage of all the agricultural land and are concentrated around the villages or towns and along the roads, of which the major affecting factors are convenience and cost of production; and 3) the land, regardless of type of land use is high in fragmentation and low in agglomeration degree and scale. It is, therefore, urgent to reconstruct the village spatial system through rearrangement of the land, and optimize the land use structure and spatial distribution of agricultural land and non-agricultural industrial land by making full use of the advantages of circular agriculture and multi-functions of the farmland so as to further raise its comprehensive land use efficiency.

To study spatial variation of plant functional type and biodiversity in various functional zones of the Hui River National Nature Reserve, vegetation survey were conducted in the core area, the buffer zone, and the experiment zone in the reserve, using the sample transects and plots method. Three plots (250 × 250 m), almost the same in habitat condition, were selected, and in each plot three transects and in each transect eight quadrats (1 × 1 m) were set up for analysis of spcies richness index, species diversity index, species diversity index,biomass and spatial variability of life-form plant functional type in different functional areas of the reserve . Results show that in the study area, the vegetation was dominated with perennial forbs and perennial grasses; in the core area and buffer zone, the vegetation was also dominated with a huge variety of perennial grasses and perennial forbs, and only dotted with some annual herbs, while in the experiment zone, the number of functional species, like perennial grasses and perennial forbs, declined markedly, but the population of annual herbs grew. Moreover, the dominance of shrubbery gradually increased from the core and buffer zone to experiment zone. The buffer zone did not differ much from the core area in dominance of perennial grasses and perennial forbs (P > 0.05); however, in the experiment zone, the dominance was significantly weakened (P< 0.05). The dominance of annual herbs gradually stepped up from the core area to the buffer zone and experiment zone. Biomass of perennial grasses and perennial forbs and total biomass of plant community in the three areas showed a declining order of core zone > buffer zone > experiment zone, however, biomass of shrubs and annual herbs increased in a reverse order. The species richness index of the buffer zone was the highest, and significantly higher than that of the experiment zone (P< 0.05),while Shannon-Wiener index and Pielou index did not vary much between the core area and buffer zone (P< 0.05).

The Giant Panda is listed on the IUCN (International Union for the Conservation of Nature and Natural Resources) as a rare endangered species. How to effectively protect giant panda and its habitats is an important part of biodiversity conservation in China. The Labahe Nature Reserve, which is located in Ya'an, west of Sichuan, is also one of the hotspots for biodiversity conservation in the world. However, due to complex terrain of the region, discrete distribution of the panda’s habitats and lack of historical research data, only few studies have been done to explore dynamic variation of the distribution of giant panda populations and their habitats. Thus, it is necessary to carry out researches of dynamics of the giant panda population in this area. Distribution of giant panda and its sympatric species in the Labahe Natural Reserve, Sichuan, was investigated from April 15 to June 18 and from October 12 to November 18 in 2010, and from May 6 to June 16 in 2011, through transect survey. Results show that (1) giant pandas in this region prefer to wander in temperate conifer-broadleaf forests and temperate conifer forests with canopy density over 0.4 on slopes below 45^o, between 1885-2821m in elevation, and  feed on Bashania fangiana; (2) during the survey, giant pandas were found mainly in four regions: Luzigou-changheba, Heixuangou, Xiaolianghekou(left), and Guanfanggou, among which Heixuangou had the most trace points and Xiaolianghekou(left) was the highest in encounter rate, and both of the parameters increased with the time passing on; and (3) the giant pandas in the reserve had mainly 15 species of companion animals, among which Cervus unicolor, Budorcas taxicolor, Macaca thibetana and Moschus berezovskii were the most seen ones, large in population and extensive in distribution; 9 species were losing in number of loci, with Budorcas taxicolor in particular; and Cervus unicolor,Naemorhedus goral,Elaphodus cephalophus,Ailurus fulgens and Capricornis sumatraensis gained in number of loci, especially Cervus unicolor; however, Selenarctos thibetanus fluctuated in number of loci and was quite stable on the whole. This research may provide scientific basis for the reserve to effectively protect Giant Pandas and its sympatric species, and for related administrators to make decisions to on building giant panda habitats and manageing the nature reserve.

Five plots of land under different types of vegetation typical of the Napahai Catchment, i.e. grassland, pine forest, bare land, 3-year revegetated land and 1-year revegetated land, were selected, and soil water content, runoff, sediment, bulk density and organic carbon content therein measured for exploration of variation of soil water regime with vegetation and its influencing factors, which is of positive significance to the realization of protection of the local fragile ecological hydro-environment and sustainable development of the regional economy simultaneously. Results show that soil water content varied sharply with vegetation, and was 50.7%, the highest, in grassland, 34.5% in 3-year revegetated land, 28.7% in pine forest, and only 20% or so in bare land and 1-year revegetated land. The five plots of land did not differ much in soil physico-chemical properties, but did in surface runoff coefficient with grassland being the lowest, 1.21% only, and pine forest being 5.73%. Grassland and pine forest was lower than bare land and 1-year revegetated land in sediment yield. Soil water content was not significantly related with any soil physico-chemical properties and sediment yield, except for bulk density. Planting grasses may rapidly expand vegetation coverage of a land, which is an effective approach to rapid amelioration of the surface soil layer in physico-chemical properties, and strengthening the soil in water holding capacity.

In order to find a solution to the ecological problem of, soil erosion in sloping farmland in coal-mining subsidence areas, a field experiment was conducted to explore effects of tillage on water use efficiency (WUE) and grain yield of summer maize. It was designed to have four tillage methods, that is, flat tillage (CK), flat tillage + stubble mulching (T1), contour ridge tillage (T2), and contour ridge tillage + stubble mulching (T3). Results show that stubble mulch lowered significantly unnecessary evaporation during the seedling and jointing stages(P<0.05). As a result, soil water content in the 0-100cm soil layers was 9.64% and 9.74% higher in T1 and T3, respectively, than in CK at the jointing stage. After the jointing stage, though there was much rain, surface run-off was much less in T1, T2 and T3 than in CK, suggesting that T1, T2 and T3 improved their water and nutrient retaining capacities. Hence, T1, T2 and T3 was also significantly higher in grian yield, WUE, rainfall use efficiency and N partial factor productivity than CK(P<0.05). The effect of T3 reducing soil nutrient and water losses was more significant than the others. Besides, T3 was 16.59%, 31.19%, 16.55% and 16.59% higher than CK in grian yield, WUE, rainfall use efficiency and N partial factor productivity, respectively. Therefore, T3, that is, contour ridge tillage plus stubble mulching,is an effective tillage method applicable to sloping farmland in coal-mining subsidence areas.

Soil samples were collected from cotton fields of transgenic cry1Ac + cry2Ab cotton and conventional cotton at each growth stage for analysis of soil bacteria and 16S rDNA gene, using the methods of conventional plate culture, Biolog analysis and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis, and impacts of the transgenic cotton on bacterial community structure and functional diversity of soil bacteria have been discussed. Determination of the population of culturable bacteria indicates that the soils in the two cotton fields shared a similar trend in variation of the population of culturable bacteria throughout the entire cotton growth period, and no significant difference existed between the two in total population of soil bacteria, except at the budding stage(P<0.05). Biolog analysis shows that the soil bacteria in the two fields differed significantly in activity and carbon utilization capacity at the seedling and bud stages(P<0.05). Cluster analysis of the PCR-DGGE profiles of the soil bacteria demonstrated that soil bacteria in both fields varied in community structure with growth stage and differed sharply only at the flower and boll stage. PCR-DGGE band sequencing analysis shows that the differences between soil bacteria of the two fields were quite random, which may be attributed to differences in sampling timing and sampling site. To sum up, soil bacteria in the two cotton fields shared a similar trend in variation of community structure and functional diversity throughout the entire growth period, and the impact of transgenic cotton was short and temporary at certain growth stages, and nill during most of the growth period.

Biological species resources are kind of strategic resources essential to sustainable development of the national economy. The research and development of a bio-species resources information system is of great significance to rational  protection and sustainable utilization of the rich bio-species resources of the country’s, as well as to improvement of the management of national bio-species resources information. In response to the requirements of the nation for protection and management of the bio-species resources and with the aid of the technologies of GIS, information network and database, a GIS-based national bio-species resources information system (NSRIS) was designed and developed, and a national bio-species resources spatial distribution database was built up using county as basic unit. The overall framework of NSRIS consists of four subsystems, that is, platform management, data acquisition and management, data query and statistical analysis, and comprehensive analysis. With this system, users can effectively collect, manage and retrieve wild vascular plants and vertebrates data and information, and make use of its statistical analysis and prediction functions investigation and evaluation of the large-scale national of regional bio-species resources distribution patterns,and their status,future trends and potential threats,and hence provide decision-makers with essential early-warning information for protection and management of the national bio-species resources.To update and upgrade NSRIS, it is essential to strengthen collection and collation of important bio-species resource data from field surveys, and long-term dynamic monitoring, and ocean biodiversity data and information, to build up a mechanism for trans-departmental sharing of bio-species resource information and to develop and integrate bio-species resources predictive and early warning models, so as to form a decision-making support system.

Based on field investigations in and remote-sensing images of the Yangtze Estuary in 1980, 1990, 2000 and 2010, and taking references of reports from international and domestic literature about potentials of wetlands, similar to those in the Yangtze Estuary in vegetation and meteorology, of removing nutrient substances, such as N and P, in the waterbody, the potentials of the tidal wetlands in the Yangtze Estuary were assessed and their changes predicted. Results show that along with the expansion of land and total wetland and changes in wetland landscape triggered by poldering, N removing potential of the tidal wetlands at the Yangtze Estuarine increased from 710.53~4 039.37 t in 1980 to 5 137.68-9 305.83 t in 2010, and P removing potential did from 64.62~408.55 t in 1980 to 886.45-1 228.08 t in 2010. In the past 30 years, N removal potential of the three dominant salt marsh vegetation outside the dike increased by 800-1 100 t, and P removal potential did by about 60-70 t. But their contribution to the total N and P removal dropped by 44-71 and 62-84 percentage point, respectively, especially Scirpus and Phragmites communities, whose contributions to the total N and P removal followed a steady downward trend. In contrast, the contribution from Spartina alterniflora, an exotic species, increased from 0% to 0% to 17%-24% and 10%-19%, respectively. The assessment may provide some scientific basis for improving water quality and protecting water sources and wetlands in the Yangtze Eestuary.

In simulated tailwaters different in concentration of CuSO₄ and EDTA-Cu, Potamogeton crispus was planted. Growth of the plants was monitored regularly and PSII effective fluorescence yield (F_v/F_m), photochemical quenching (q_P) and non-photochemical quenching (q_N) of photosynthesis of the plants were determined with a underwater saturation pulse chlorophyll fluorescence analyzer (DIVING-PAM) to explore ecological response of the plants in purifying low concentration copper containing tailwater . Results show that P. crispus could adsorb and enrich copper ions, the capacity of absorbingmore CuSO₄ is higher than of EDTA-Cu. CuSO₄ was less toxic to the plant and more or less conducive to its the growth of the plant. In CuSO₄ treatments when CuSO₄ concentration was 0.01, 0.3, 0.5 and 1.0 mg·L^-1, the number of leaves per plant was 292.5%, 390.0%, 155% and 45% ,as many as that in the control, respectively. However, EDTA-Cu did not have much effect or much toxicity on growth of the plant when it was within the range of concentrations in the test. With rising concentration of CuSO₄ and EDTA-Cu, R_ET, F_v/F_m, q_P and q_N varied significantly indicating that a certain concentration of copper ion can increase the transfer rate and share of photoelectrons and promote photosynthesis and is quite low in toxicity to PSII reaction center of the plants as well.

Concentrations of As, Pb, Cr, Cd and Hg in soil and vegetable in a sewage-irrigated area in Bengbu, Anhui Province were monitored and analyzed. Heavy metal pollutions of the soil and vegetable produced therein were evaluated using the single factor index and Nemero index of pollution, and health risk of the vegetable was assessed using the EPA MMSOILS model. Results show that the average concentration of As, Pb, Cr, Cd and Hg in the soil was 4.182, 3.685, 1.397, 0.381 and,0.151 mg•kg^-1, respectively. The concentration of Cd was well over the criterion in Grade II of the Standard for Soil Quality (GB 15618-1995) (6.5＜pH＜7.5), with its single-factor pollution index reaching 1.27. The concentrations of Hg, Pb, Cr, Cd and As in vegetable were in the range in compliance with the Food Sanitation Standard (GB 2762-2012), which suggests that the vegetable produced in the sewage irrigated area is not risky. The heavy metals detected in the vegetable displayed an order of Pb＞As＞Cr＞Cd＞Hg in terms of content. Among the heavy metals in vegetable, As was the highest in risk, reaching 1.12 in hazard quotient, while the others were 0.40, 0.07, 0.12 and 0.06, separately, displaying an order of As＞Pb＞Hg＞Cd＞Cr.

Forms and contents of solid-phase phosphorus in pig and dairy cow manures before and after subjection to anaerobic digestion were analyzed, using the sequential extraction method with H₂O, NaHCO₃, NaOH and HCl. Results show that in the effluent slurry from pig dung digesters, HCl-P accounted for 85.50% of the total solid-phased phosphorus (TSP), significantly higher than that in the influent material (P<0.05);.whereas NaHCO₃-P, residual P, H₂O-P and NaOH-P did for 5.83%, 4.71%, 2.15% and 1.81%, respectively, all lower than their corresponding one in the influence to a varying extent; and in the effluent slurry from dairy cow manure digesters,. HCl-P, NaHCO₃-P, H₂O-P, residual P and NaOH-P accounted for 53.73%, 19.62%, 12.66 %, 8.60% and 5.39%, respectively, which did not differed much from that in the influent manure. The inorganic phosphorus in the slurry accounted for 60.98% - 100.00% of TSP. After anaerobic digestion, solid-phased phosphorus of various forms in the digestion sludge from the pig dung digesters displayed an order of HCl-P > NaHCO₃-P > residual P > H₂O-P > NaOH-P in content, each accounting for 87.43%, 5.17%, 4.33%, 1.79% and 1.28%, respectively, in percentage of TSP, respectively; while a different order was found in the digestion sludge from the dairy cow manure digesters, that is, HCl-P > NaHCO₃-P > H₂O-P > residual P > NaOH-P, each accounting for 69.74%, 9.91%, 8.75%, 8.30% and 3.30%, respectively. After anaerobic digestion of pig dung, liquid-phased phosphorus was transformed into solid-phased one and water-soluble P in the latter turned into insoluble P. However, phosphorus in digested dairy cow slurry was less available to plants than that in digested dairy cowpig dung slurry.

Field experiments were conducted in Jilin, Zhejiang and Guangxi provinces for determination of residual tebuconazol and azoxystrobin in tissues of rice (Oryza sativa) using GC-MS and dissipation dynamics, accumulation and distribution of tebuconazol and azoxystrobin in different parts of rice explored. Results show that dissipation dynamics of tebuconazol and azoxystrobin residues in the plants fitted the first-order kinetic equation, and dissipation half-life of tebuconazol and azoxystrobin in the plants was 4.68-5.68 and 4.65-6.08 d, respectively. When 168.8 g•hm^-2 (recommended dosage) and 253.2 g•hm^-2(1.5 times the recommended dosage) of 750g•L^-1 tebuconazol and azoxystrobin water dispersible granules was sprayed twice and thrice, respectively, the accumulation and distribution of tebuconazol and azoxystrobin in tissues of the plants was characterized by the order of husk > straw > rice in Jilin and the order of straw > husk > rice in Zhejiang and Guangxi.

With β-actin as reference genes and by means of the real-time fluorescence quantitative RT-PCR method, levels of mRNA expression of HSC70 and HSP70 in gill, mantle and digestive gland of Crassostrea hongkongensis exposed to NP varying in concentration (0, 0.01, 0.1, 1, 10, 100 and 300 μg•L^-1) were determined at 12, 24, 48, 96 and 192 h. Results show that 0.01 μg•L^-1 NP induced significantly expression of HSC70 and HSP70 mRNA in gill and disgestive gland(P<0.05). With rising NP concentration in treatment, the expression level increased gradually. Within a certain time period, 100 μg•L^-1 NP induced significantly a higher expression level than 300 μg•L^-1 NP did(P<0.05). When the expression of HSC70 gene in gill and digestive gland peaked at 96h, it did at 48h in mantle, and when the expression of HSP70 in gill peaked at 48h, it did at 24h in mantle and digestive gland. It is quite obvious that HSC70 and HSP70 genes in the oysters respond significantly to NP.

In order to explore relationship between chlorophyll-a and water quality factors in Macrobrachium rosenbergii rearing ponds, during the period from June to October in 2011, water quality parameters such as temperature (T), dissolved oxygen (DO), pH, transparency, nitrite nitrogen (NO₂-N), ammonia nitrogen (TAN), total nitrogen (TN), reactive phosphorus (PO₄-P), total phosphorus (TP), chlorophyll-a (Chl-a) and chemical oxygen demand (COD_Mn) in waters of 3 Macrobrachium rosenbergii Ponds and their water source in Jinshan District, Shanghai were monitored, and for the relationship of Chl-a with the other water quality factors was analyzed using the grey correlation analysis method. Results show that the correlation degree of Chl-a with the other factors displayed an order of TN＞TP＞COD_Mn＞DO＞pH＞NO₂-N＞T＞TAN＞PO₄-P＞transparency, thus demonstrating that TN, TP, COD_Mn, DO and pH were the main ones and TN and TP the most important ones affecting water quality in Macrobrachium rosenbergii ponds.

A total of 175 soil samples were collected from surface soil layers at Yushu and Maduo, in the three rivers source region (Yangtz River, Yellow River and Lancang River) from July to September in 2012 for analysis of the chemical properties and Hg contents.On such a basis, relationships of Hg content with soil pH and total organic carbon (TOC) were investigated, and distribution characteristics of mercury in the soil of Yushu and Maduo were discussed. Results show that the soil in the studied area tended to be slightly alkaline and rich in TOC. The soil in Maduo was is higher in pH, but slightly lower in TOC than the soil in Yushu. The arithmetic average of mercury contents in the soil of the studied area was 0.034 mg•kg-¹, which was lower than the average backrground value of the country, but obviously higher than the average background value of Qinghai Province in 1990. Soil Hg content in the studied area was obviously on the high side, and much higher in gray-cinnamon soil than in the other four types of soils, which differed very slightly in average Hg content. The spatial distribution of Hg contents demonstrates that Hg content in the surface soil in Yushu was much higher than in Maduo, which is related to degree of urbanization and industrial structure of the two areas. High soil Hg content was found mainly in the areas with dense population and busy traffic. Correlation was found between Hg content and pH, while significantly positive relationship was observed between Hg content and TOC (r = 0.605, n = 90, P<0.05). Compared with Yushu, Maduo, relatively higher in pH and lower in TOC, was lower in soil Hg content, of which the specific cause waits needs to be further studied through continuous observation of changes in soil environmental quality in the area.

Effects of exposure to different concentrations of benzo(a)pyrene(BaP)(0.1,1,10 and 50 μg•L^-1)on cytochrome P450 1A1 (CYP 1A1) and glutathione S-transferase (GST)activities in liverof tilapia were studied.Results show that the exposure to BaP, 0.1,1 or 10 μg•L^-1 in concentration did not have significant effect (P > 0.05) on CYP1A1 activity, but when the concentration rose up to 50 μg•L^-1, CYP1A1 activity was significantly induced after 6 or 168 h of exposure (P < 0.05). Likewise, the exposure to BaP, 0.1 or 1 μg•L^-1 in concentration did not have significant effect on GST activity in liver(P > 0.05), and when the concentration rose up to 10 or 50 μg•L^-1, it did after 336 h of exposure (P < 0.05). The study on relationship between CYP1A1 and GST in activity reveals a similar trend in variation, especially in groups with BaP concentration being 1 or 50 μg•L^-1, suggesting that there exist  certain correspondence between CYP1A1 and GST activities.