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Table of Content

Volume 30 Issue 6
25 November 2014
Resilience Concept Analysis and Evaluation of Socio-Ecological Systems: An Overview
LI Xiang-Mei, XIAO Ren-Bin, WANG Hui-Li, LIU Zhi-Ling
2014, 30(6):  681-687. 
Abstract ( 808 )   HTML ( 165)   PDF (912KB) ( 4610 )  
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How a socio-ecological system (SES) responds to changes in global environment has been deemed as a priority concern in the modern natural resources management. The resilience theory provides an important theoretic basis to scientific analysis of interactions between disturbance and adaptivity in a SES. With the contradiction between human and land resources getting increasingly prominent,, it is of great significance to analyze the connotation of resilience as a valuable feature of the nature-human system and to sum up progresses in the research of its evaluation. Based on the introduction to genesis of the resilience theory, definition of resilience was dissected and explanations of engineering resilience, ecosystem resilience and socio-ecological resilience were tried, with emphasis on analysis of their differentiation from and relationships with frangibility, adaptability and sustainability. Meanwhile, a review was presented of quantitative evaluation and application of resilience from the angle of framework, indices and tools of the evaluation. In the end, for better understanding of the resilience in SESs outlooks on the future development tendency of the resilience are described in the aspects of connotation of resilience, quantitative evaluation and remediation practice of the degraded SESs.

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Ecological Security Evaluation of National Important Ecological Function Areas
ZOU Chang-Xin, XU Meng-Jia, GAO Ji-Xi, YANG Shan-Shan
2014, 30(6):  688-693. 
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The "National Ecological Function Zoning"of China defined 50 national important ecological function areas, which have great significance on the protection of national and regional ecological security. Indicators were selected from two aspects of ecological stress and ecosystem structureto form ecological security evaluation system, and zoning ecological security evaluation based on remote sensing data of three periods were conducted on national important ecological function areas in order to identify areas where ecological security conditions show deterioration trends, which could provide theoretical and scientific basis for ecological security assurance of national important ecological function areas. Results show that ecological security indices of water conservation areas and biodiversity protection areas were relatively high, while soil conservation areas were in poor situation. Overall ecological security situation of national important ecological function areas has been declined. The ecological environment situation of Dongting Lake flood regulation areas and the southeastern Tibet mountain rainforest biodiversity protection areas improved significantly. While the ecological security index of the wind prevention and sand fixation areaslocated in the middle and lower reaches of Heihe River were the least and had a further declining trend.

Study on the Fairness of Resource-Environment System of Anhui Province Based on Ecological Carrying Capacity of Per Capita
LIU Feng, ZHANG Yun, GU Chang-Mei, XU Sheng
2014, 30(6):  694-698. 
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Through expansion of the connotation of “per capita ecological carrying capacity”, the concept of ecological Load coefficient (ELC) was established. Based on multiple time sections, the ecological carrying capacity per capita of 17 cities in Anhui Province during 2001-2010 was calculated. At the same time, based on the per capita ecological carrying capacity the energy consumption, fresh water consumption, COD discharge and SO2 emission were chosen to establish the methods for calculation and evaluation of the Gini coefficient of resources and environment (Gr,e) and the ELC in Anhui province. The results show that (1) The SO2 emission of Gr,eare higher than the “red line” of 0.4, and the energy consumption of Gr,e is close to 0.4. The Gr,e of other items such as fresh water consumption and COD discharge are all in a relative and reasonable scope. (2) On the whole, the resource-based cities (such as Ma'anshan, Huainan), the relatively developed cities (such as Hefei) and the industrial cities along the Yangtze River (such as Anqing, Wuhu)in Anhui Province are the main factors contributing to unfairness of the resource consumption and pollutant release. These cities need to optimize the allocation of resources and environment. Cities in the mountainousareaof south Anhui Province (such as Huangshan, Chizhou, Xuancheng) are the main factors contributing to fairness of resource consumption and pollutants release. The results of thestudy can provide a theoretical basis for safety assessment and fairlydistribution of the resources and environment  of Anhui Province.

Environmental Health Risk Assessment of Phthalic Acid Esters Like Pollutants in Reservoir or Lake Type Water Source Catchments in the Pearl River Basin
HE Tao, XU Zhen-Cheng, WEI Dong-Yang, BAI Zhong-Yan, REN Ming-Zhong, BAI Xiao-Jian
2014, 30(6):  699-705. 
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Water samples were collected along the tributaries of a drinking water source catchment for analysis in line with the procedure for risk assessment of the USEPA, and environment health risk of phthalic acid esters (PAEs) was assessed by taking into account the parameters of the region. Results show that (1) all the samples were found to have PAEs like pollutants, including Di (2-ethylhexyl) phthalate (DOP), diethyl phthalate (DEP), di-n-butyl phtalate (DBP), among which DOP was higher than the other two, displaying an order of DOP > DEP > DBP.  (2) The concentrations of DOP, DEP and DBP in the water catchment were all higher than their respective ones in other drinking water source catchments in the region and could also be sorted into the moderate or upper level of contamination in similar areas in the country and others. DOP was designated the primarily controlled contaminant of the water source catchment.(3) In areas less affected by anthrogenic disturbance, environmental health risk of pollutants in rivers was low, while in areas densely populated and concentrated with industry, the risk was high. Along a river, the risk was low in the upper reaches, high in the middle reaches and low again in the lower reaches or the reservoirs.Therefore, it can be concluded that the environment health risk of PAEs like contaminants in headwater catchment has not yet exceeded the criteria of USEPA, but among similar regions the world over, this region is moderate or high in concentration of PAEs like contaminants and does have some potential health risks. It is, therefore, essential to track potential source of PAEs in the region and take corresponding countermeasures to control the sources in environmental risk management of the region.

Status Quo of Collection and Utilization of Rice and Wheat Straw in Jiangsu Province and the Countermeasures
ZHANG Si-Mei, YANG Si-Jun, SHI Zu-Liang, CHANG Zhi-Zhou
2014, 30(6):  706-710. 
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A case study of Jiangsu Province was conducted using questionnaires and field survey to explore status quo of the collection and utilization of rice and wheat straw of the province. Problems in collecting the straw were analyzed, taking into account meteorological data of the rice and wheat harvesting seasons in the region. Results show (1)currently the major straw utilization method is to incorporate straw directly into the field. In the two townships (A and B) investigated in the study, only 22.01% and 28.75%, separately,of the farmer households interviewed had followed the practice and both of the two townships had a considerable portion of the straw wasted or improperly disposed. In Township B over 50% of the farmer households surveyed failed to make use of any of the straw and as high as over 55% of the straw was discarded or burnt. (2) During the rice and wheat harvesting seasons, precipitation varies in the range of 7.21-87.28 mm and in every 10 days, there were1.53-5.00 rainy days. Such weather not only affectsthe timely harvesting of rice and wheat, but also seriously impedes baling, transportation, and storage of straw.(3) As the crops must be harvested in a relatively short and concentrated time period, large volumes of straw are turned out within a few days. Moreover, harvesting of the first crop often coincides with sowing of the next crop, leaving little time available for straw collection. (4) Straw collection is very low in economic benefit. A farmer can only get 60-90 RMB per day from straw collection and transportation, much lower than what they can get by working as migrant workers in city.(5) Machines designed for straw collection are rare and those now used in the operation are low in efficiency and need to be improved technically, which directly affects straw collection efficiency. The above-listed findings indicate that time shortage, negtive weather condition, low benefit and low mechanization level are the major factors affecting straw collection. To solve the problems, the following proposals are brought forth, that is, improving the economic benefit of straw collection as a driving force,accelerating development of rice and wheat sheaf-binding reaping machine,and setting up long-term operational mechanism for straw recovery, in the hope that this study may provide some useful ideas to help solve the problems.

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An Improved Agricultural Production Function Model and Contribution Analyses of Genetic Resources to Agricultural Economic Growth in Jiangsu Province
WU Jiang-Yue, PU Li-Jie, ZHU Ming, DAI Xiao-Qing, CHEN Xin-Jian, HAN Ming-Fang
2014, 30(6):  711-716. 
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Genetic resource is an important input factor in agricultural production. Usually contributions of production factors such as labor, fixed assets and technology to agricultural economic growth were calculated using the C-D production function model. While in this study, the concept of genetic resources was firstly introduced into this model to evaluate contributions of the factors to agricultural economic growth. For that end, Jiangsu Province was cited as a case for study. Calculation of contributions of genetic resources and other production factors to agricultural economic growth was done based on the data of agricultural production in Jiangsu Province from 2001 to 2007. Results show that agricultural economic growth in Jiangsu Province depends mainly on input of animal genetic resources and technological innovation. Although the contribution of technological innovation to agricultural economic growth in Jiangsu Province is high, while that of genetic resources is also significant. Meanwhile, the contribution of animal genetic resources is larger than that of plant genetic resources. Therefore, Jiangsu Province should optimize its agricultural structure, improve its utilization of genetic resources, and increase its sci-tech input so as to promote its construction of its modern agriculture.
Identification of Sediment Sources Using Composite Fingerprinting:Selection of Potential Sediment Sources
CHANG Wei-Na, ZHOU Hui-Ping, GAO Yan
2014, 30(6):  717-723. 
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The application of the technique of composite fingerprinting to the identification of sediment sources is based on the assumption that the type of potential sediment sources could be confirmed and the sum of the contributions of the sediment sources equals to 1. However, the uncertainty in prediction of potential sediment sources would have some impact on accuracy of the results. The upstream of the Jiuxiang River in Nanjing was investigated for case study. Mean significance tests were carried out of fingerprint factors of the sediment sources in order to test whether each predicted source could be used individually as a potential sediment source. Meanwhile, effects of different sediment sources on the accuracy of the determination based on composite fingerprinting were analyzed and compared, in an attempt to quantitatively analyze shortages in the process of sediment source prediction and explore for ways to mitigate the impact of sediment source selection on identification of sediment sources. Results show that the four predicted sediment sources were readjusted as farmland, woodland and road. The readjustment significantly enhanced  the differences in fingerprint factors between the sediment sources. The highest identification rate of 89.2% was obtained in discriminating sources by using optimum composite fingerprinting. In terms of relative contribution of sediment, the sediment sources displayed an order of farmland (39.9%~87.8%) > wood land (<0.1%~47.7%) > and road land (0~25.6%).

Effect of Leaf Litter Water Extract of Three TreeSpecies on Growth and Resistance Physiology of Bidens pilosa
LIAO Meng-Yu, HU Ting-Xing, DENG Cheng-Min, WANG Qian, LONG Hua, BAI Yi
2014, 30(6):  724-730. 
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As an invasive plant, Bidens pilosa imposes great potential harm to agricultural production, biodiversity and safety of ecosystems. Making use of allelopathy to develop new herbicides, is a meaningful way to control and reduce negative impact of chemical herbicides to the environment. It has been reported that leaf litters of common species, such as Eucalyptus grandis, Cinnamomum camphora and Juglans regia, could generate secondary metabolites during their growing process, which show comparatively strong allelopathy to plants and microorganism, and might play an important role in the prevention of colonial invasion herbs. Selected leaf litters of E. grandis, C.camphora and J.regia,were made into different concentrations of water extracts. By combining the methods of indoor germination test and potted biological test,  indexes such as germination rate, germination speed,  aerial parts fresh weight,  antioxidant enzyme activity, and osmotic regulation substance of B. pilosa were determined. Finally, comprehensive evaluation and comparison of allelopathy effects of the three species were completed. The water extracts of E. grandis leaf litter significantly inhibited the germination rate and germination speed of B. pilosa, while C. camphora and J. regia showed weaker inhibition. The leaf litter water extracts of the three tree species promoted the activity of peroxidase (POD), superoxide dismutase (SOD) and inhibited the activity of catalase (CAT) in B. pilosa. The antioxidant enzyme system of B. pilosa treated withE.grandis leaf litter extract showed passivation response, and thus lead to obvious increase of malondialdehyde (MDA). When treated with leaf litter extracts of E. grandis and C. camphora, the soluble protein and soluble sugar in B. pilosa increased with increasing extract concentration.However, when  treated by leaf litter extracts of J. regia, the soluble protein and soluble sugar in B. pilosa showed a trend of promotion at low concentration, and inhibition at high concentration. Overall, the leaf litter of three tree species showed potential allelopathic effects on the germination and growth of B. pilosa, and led to significant changes in resistance physiological indicators in B. pilosa, but the effects varied by the tree species and the extract concentration of leaf litter. Among them, E.grandisdemonstrated the strongest inhibition on B. pilosa, followed by C.camphora, and J. regiais the weakest.

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Population Size and Distribution Patterns of Captive Red-crowned Cranes (Grus japonensis) in Zoos in China
ZHOU Da-Qing, WANG Zhi, GAO Jun, ZHANG Hao-Nan, JIANG Ming-Kang
2014, 30(6):  731-735. 
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A survey was carried out on population size of captive red-crowned cranes in 141 zoos (including urban zoos,park zoos,and wildlife parks) in China, through telephone consultation, field investigation and web searching. It was found that 83 of the 141 zoos had red-crowned cranes in captivity, with a total number of 917, of which 319 were in urban zoos, 82 in park zoos and 516 in wildlife parks. Differences between regines and between zoos (parks) were quite obvious in distribution of captive red-crowned cranes. One-way ANOVA shows that wildlife parks had more captive red-crowned cranes than park zoos did, but the differences were not so significant between urban zoos and wildlife parks/park zoos. The top three provinces in number of captive Red-crowned Cranes were Liaoning, Guangdong, and Heilongjiang, and the top ten provincial level administrative regions had a total of 719 captive red-crowned cranes, accounted for 78.4% of the total number in China. The top three regions in number of captive red-crowned cranes were Northeast China, East China, and North China, and had captive red-crowned cranes accounting for 74.3% of the total number in China.
Effect of Aquatic Plants on Emission of Gases From Eutrophic Water
ZHANG Li, ZHANG Zhen-Hua, GAO Yan, YAN Shao-Hua
2014, 30(6):  736-743. 
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Denitrification and emission of greenhouse gases and oxygen are all important biochemical processes occurring in aquatic ecosystems. To define extent of the impact of aquatic plants, relative to type, on these processes, dynamics of N2, CH4 ,O2 ,CO2 and N2O emissions from eutrophied waters in Caohai of Lake Dianchi, rank with emergent plants (Acorus calamus), floating-leaved plants (Nymhaea tetragona, Nelumbo nucifera), floating plants (Eichhornia crassipes) and submerged plants (Myriophyllum verticillatum, Hydrilla verticillata) and in control (waters without plants) were studied in July and August, 2012. Gases released from the waters were in-situ collected using a self-developed bubble trapping device, while water temperature, dissolved oxyen(DO), oxidative redox potential(ORP) and pH were also measured. Results show that the gas emitted from the waters with or without aquatic plants, were composed mostly of N2, CH4 and O2, and the rest of CO2 and N2O. The submerged plants obviously improved O2 releasing process via photosynthesis, resulting in higher gas emission rate and higher emission fluxes of various fractions of gass. The leaves of Nymhaea tetragona, Nelumbo nucifera, and Eichhornia crassipes created shadow over the water surface, thus decreasing photosynthetic capacity of algae in the water, and consequently the gas emission rate of the water, with its median being even lower than that in the control. Moreover, the shadow also reduced emission fluxes of various fractions of gases. No significant difference was found in CH4 concentration in emitted gas between the experimental plots, while the concentration of N2 in the gas emitted from the waters grown with Acorus calamus or Nymhaea tetragona was significantly higher than that from the control. The study provides a new method and idea for studying how different aquatic plants affect the gas releasing process in waters.  
Nitrogen Removal of Polyvinyl Alcohol(PVA) Gel Embedded With Nitrifying/Denitrifying Bacteria Coupled With Plant
ZHANG Yong-Dong, WANG Long-Mian, ZHANG Yi-Min, GAO Yue-Xiang, YANG Fei, CHE Yu-Xiao, ZHOU Chuang, LIU Qi-Gen
2014, 30(6):  744-748. 
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To explore effect of polyvinyl alcohol(PVA) gel embedded with nitrifying/denitrifying bacteria in remedying ecology of eutroficated waterbody, a 21-day N-removal  experiment was conducted using the gel coupled with two species of plants (Oenanthe clecumbensand Ipomoea aquatica). Results show that the concentration of TN, NH4+-N, NO3--N, NO2--N and TP in the group added with bacteria was significantly lower than that in the group without bacteria, respectively(P<0.05). Correlation analysis shows that nitrogen removal efficiency in the experimental groups was significantly and positively related to the addition of nitrifying bacteria and denitrifying bacteria embedded in PVA Gel(P<0.05). The mean growth rate of Oenanthe clecumbensand Ipomoea aquatica was 38.5 and 19.6 mg·g-1·d-1, respectively, in the experimental group added with the gel(RGR)and 29.9 and 9.5 mg·g-1·d-1, respectively. in the group without the gel.
Adsorption and Leaching of Sodium Penicillin in Soils
ZHANG Zhen-Hua, MA Shan-Shan, LIU Yan, YU Ci-Gang, YU Ran, WANG Chang-Yong
2014, 30(6):  749-753. 
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Adsorption and leaching of sodium penicillin in soils and their affecting factors were studied with the vibrating equilibrium method and the column leaching method. Results show that adsorptions of the substance in red soil from Jiangxi, paddy soil from Taihu and black soil from Northeast China at 25℃ or below, were fairly well fitted with the Freundlic model with adsorption constant being 30.73, 39.26 and 64.58 mL·g-1, respectively. PLS (partial least squares) analysis reveals that soil clay and organic matter were the key factors affecting soil adsorption of sodium penicillin in the soils. Free energies of sodium penicillin adsorption in all the three soils were less than 40 kJ·mol-1, suggesting that the adsorption belonged to the type of physical adsorption. The soils followed an order of Northeast China black soil > Taihu region paddy soil > Jiangxi red soil in terms of sodium penicillin adsorption capacity. The leaching experiment indicates that the content of soil clay and the content of organic matter are also the two factors affecting leaching of sodium penicillin in soils, and that the three soils followed an order of Jiangxi red soil > Taihu region paddy soil > Northeast China black soil in terms of sodium penicillin movability in the soil profiles.
Anionic/Nonionic Surfactant Enhanced Microscale Cu/Fe Bimetal Degrading Chlordane and Mirex in Water and Soil Slurry
WAN Jin-Zhong, MA Yu-Ping, ZHANG Sheng-Tian, LONG Tao, LIN Yu-Suo, LI Qun, MIN Jie, LI Chuan
2014, 30(6):  754-760. 
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Chlordane,endosulfan and mirex were selected as target pollutant in bench scaled experiments using surfactant-enhanced microscale Cu/Fe bimetal to degrade organochlorine pesticides (OCPs) in water and soil slurry. Results show that Triton X-100 (TX-100), a nonionic surfactant,exhibited higher stimulative effect on Cu/Fe reactivity than sodium dodecyl sulfate(SDS), an anionic surfactant, did. The TX-100-Cu/Fe system could degrade OCPs in water rapidly and efficiently.The optimal TX-100 dosage was 0.1 mmol·L-1. Twelve hours after treatment, the degradation rate of γ-chlordane,endosulfan and α-chlordane reached nearly 100%, and that of mirex was also as high as 85.2%. In treating soil slurry, acidic pH was very important to a highreducing activity of the microscale Cu/Fe particles. The addition of TX-100 greatly promoted the effect of microscale Cu/Fe degrading OCPs in soil slurry. A higher proportion of Cu in the bimetal system led to enhanced removal of contaminants. When a soil/water suspension, 1:5 in ratio,was amended with 10% of Fe and loaded with 5.0% of Cu and 5.0 mmol·L-1, TX-100, 72 h of incubation in pH 4 – 5 had 83.5%, 68.1%, 86.8% and 70.1%, respectively, of the γ-chlordane, endosulfan, α-chlordane and mirex in the suspension degradation. The findings suggest that the TX-100 enhanced microscale Cu/Fe offers an efficient method for remediation of OCPs contaminated soils.
Remediation of 3,5,6-Trichloro-2-Pyridinol Polluted Soil With Ralstonia sp. T6 and Construction of a Chlorpyrifos-Mineralizing Engineered Strain
ZHANG Yang, WANG Fei, HUANG Yan, CUI Zhong-Li
2014, 30(6):  761-767. 
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TCP is a kind of metabolic product of degradation of chlorpyrifos. Ralstonia sp. T6, a TCP (3,5,6-trichloro-2-pyridinol) degrading strain of bacteria, was used in an experiment to explore its TCP degrading characteristics in soil. Results show that T6 degraded 80% of 50 mg·kg-1 TCP in 6 days. Temperature, inoculation rate and initial concentration of the substrate were factors affecting its degrading efficiency. The optimum temperature for T6 degrading TCP was 30℃; in soils lower than 10×108 CFU/kg in bacteria content, TCP biodegradation rate rose with rising bacteria content, while in soils higher than that, the rate did not rise, but fall instead with rising bacteria content. A methyl parathion hydrolase gene (mpd) from Stenotrophomonas sp. DSP-1 was inserted into 16S rDNA of Ralstonia sp. T6 by conjugation and a genetically engineered T6-mpd that is supposed to be able to completely mineralize chlorpyrifos was obtained. Culturing tests show that T6-mpd and Ralstonia sp. T6 displayed similar growth characteristics. Chlorpyrifos degrading tests show that in LB medium, the chlorpyrifos hydrolysis efficiency of T6-mpd was basically the same as that of Stenotrophomonas sp. DSP-1, but in inorganic salt medium, T6-mpd‘s 50 mg·L-1 chlorpyrifos hydrolysis rate in 60 h reached only 36%, significantly lower than DSP-1’s. Simulated in-situ soil remediation shows that T6-mpd, 10×108 CFU·kg-1 in content, could degrade 64% of 50 mg·kg-1 chlorpyrifos. It is, therefore, believed that Strain T6-mpd is a promising tool for bioremediation of chlorpyrifos-contaminated environment.
Effect of Pennisetum hydridum Degrading Atrazine in Soil
CHEN Jian-Jun, ZHANG Kun, LI Ming-Rui, LI Yuan
2014, 30(6):  768-773. 
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A pot experiment was carried out in order to understand effects of Pennisetum hydridum on atrazine degradation, dynamics of the degradation and translocation of the substance, and relationships between concentration of atrazine residue and activities of relevant soil enzymes in the soil. Results show that atrazine degradation rate significantly increased in pots planted with P. hydridum. by 52.84 percentage in non-sterilized pots and by 42.38 percentage in sterilized pots. The half-life of soil atrazine in sterilized and non-sterilized pots planted with P. hydridum was significantly shortened, by 64.35 days and 53.21 days, respectively. Atrazine in soil was absorbed by P. hydridum, and then gradually transferred from roots to shoots. With the plants growing on and on, transfer coefficient of the substance increased. Significant negative relationships were observed between concentration of atrazine residue in the soil and activities of soil catalase, peroxidase, invertase and polyphenol oxidase. All these findings indicate that planting P. hydridum is conducive to degradation of atrazine in the soil.
Accumulation and Transfer of Lead (Pb) and Cadmium (Cd) on Different Species of Maize
YANG Wei-Wei, LIU Min, CAO Mei-Zhu, ZHANG Chao-Lan
2014, 30(6):  774-779. 
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Based on a pot experiment using 10 cultivars of maize from 10 difference production areas, characteristics of the maize adsorbing and accumulating heavy metals, like Pb and Cd were studied. Results show that under combined Pb (400.0 mg · kg-1)and Cd (10.0 mg ·kg-1) stress, the accumulation of Pb in maize varied with the growth stage, displaying decreasing order of ripening stage>jointing stage>seedling stage, while the Cd accumulation in maize did, too, but displaying a different order, i.e. seedling stage > jointing stage > milking stage. In addittion, the distribution of Pb and Cd in the plant also varied, showing an order as root > stem > leaf > grain, which means that root was the main part where the heavy metals were accumulate. Obviously, the 10 cultivars of maize all exceeded the criteria for Pb (≤0.2mg· kg-1) in grains specified in the National Sanitation Standard of China (NSSC),in content of Pb, while Cultivars Guangtian 3, Guoshenjun 79-5, Xinghuangdan 892, Jindan 51, and Beike were under the criteria for Cd (≤0.1mg· kg-1) in grain specified in the NSSC. The lowest accumulation of Cd was found in Guangtian 3, which suggested that this maize cultivar can be extraploted to areas slight or medium in soil pollution.
Effect of Glucose Addition on Biogas Production of Wheat Straw
CHEN Guang-Yin, CHANG Zhi-Zhou, DU Jing, YE Xiao-Mei, XU Yue-Ding, ZHANG Jian-Ying
2014, 30(6):  780-784. 
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To explore inner mechanism of mixed anaerobic fermentation, an experiment was conducted to determine effects of glucose amendment rate and method on biogas production of wheat straw under batch anaerobic fermentation at (35±1)℃, using wheat straw as raw material and glucose as extraneous easily biodegradable organic carbon. Results show that in the initial period of the experiment, addition of glucose increased daily biogas yield, but when the glucose addition rate was too low, the effect was negligible, and when the rate was raised up to 6%of TS (total solid weight) of wheat straw, biogas yield per unit of TS of wheat straw was peaking to 303.13mL·g-1. But further increase in glucose amendment rate affected reversely daily biogas yield. Once daily biogas yield began to decline, the amendment of glucose, either once for all or in several times, had no promoting effect on daily biogas yield.Only at the beginning the experiment, glucose amended at a rate of 6% of TS of the wheat straw would have some promoting effect on biogas production on wheat straw under anaerobic fermentation, raising the daily biogas yield per unit of TS by 9.24% as compared with CK. Addition of glucose did not have much effect on content of methane during the process of biogas production from anaerobic fermentation of wheat straw, but greatly raised mean concentration of cumulative biogas production, and the effect was more significant during the daily biogas yield declining period. However, such a promoting effect was not significant as compared with CK. To sum up, the amendment of glucose at a rate of 6% of TS of wheat straw once for all at the beginning of the experiment can achieve the highest biogas yield.
Screening of Pesticides for Priority Control for the Sake of Protecting Aquatic Organisms in the Yangtze River Delta Basin
CHENG Yan, ZHOU Jun-Ying, SHAN Zheng-Jun
2014, 30(6):  785-794. 
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For protection of aquatic organisms in the Yangtze River Delta Basin, it is essential to define principles for screening out pesticides for priority control. Based on the prediction with models, pesticides commonly used in the region were screened in light of their predicated risks to aquatic organisms, and a pesticide priority control list involving 15 pesticide active ingredients was worked out. The list could provide a priority list for formulating a national surface water ecological criteria for pesticides, and also serve as guidance for control of pesticide pollution in surface water bodies in the Yangtze River Delta Basin.
Changes in Land Use/Cover Change and Fragmentation of Landscape in the Kaidou-Kongque River Valley  in Recent 20 Years
DU Qing, XU Hai-Liang, LING Hong-Bo, WANG Xi-Yi, ZHAO Xin-Feng, ZHANG Peng
2014, 30(6):  795-799. 
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Based on Landsat-TM and CBERS images of the Kaidu-Kongque River valley in 1990, 2000 and 2010, and with the aid of the GIS technology, dynamic changes in land use/cover and landscape pattern of the valley and causes of the changes in the recent 20 years were analyzed,  Results show:In 1990 — 2010, the changes in farmland, construction land and water wetlands were the most obvious, being at a rate of 4.76%, -1.61% and 1.45%, respectively, in annual mean, while the change in virgin land, the least, being at a rate of -0.29%; 8.71% of the grassland area and 1.73% of the virgin land transformed into farmland, 2.41% of the grassland into water surface or wetland and 54.74% of the construction land into waste land; Before the year of 2000 forest land decreased in area, being mainly transformed into farmland, while after that year, farmland and grassland were turned back into forest land, increasing the forest land in area; During the time period covered by the study, cultivated land and water surface/wetland tended to be concentrated in distribution and gradually became the dominant types of landscape in the study area; Before 2000 forest land distributed in fragments, but after 2000 it began to tend to be concentrated; during the period, grassland decreased in area and in landscape dominance as well; and as a result, the landscape pattern tended to be simple.  It is believed that it is mainly the continuous growing population and their activities for seeking economic profits that has caused farmland steadily diminishing in area in the valley, thus seriously threatening the eco-environment safety of the valley.
Eco-service Functions Based on Land Transfer Matrix:A Case Study of Xianlin, a New Urban Area in Nanjing
ZHANG Yi, LI Yu-Feng, GAO Hong, LIU Hong-Yu
2014, 30(6):  800-805. 
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 A case study was carried out of Xianlin of Nanjing, a newly developed urban area for analysis of changes in land use pattern with the transfer matrix method, and classification and evaluation of eco-services with the experts grading method and AHP method, of which the results were presented spatially for further analysis of effects of changes in land use on eco-service functions of the area. The results show that(1) during the decade from 2003 to 2013, in the Xianlin region, the areas of farmland and water surface dropped by a large margin, while those of construction land, intensive grassland and bare land expanded remarkably, signifying changes in land use were embodied mainly in alienation of the former into the latter; (2) eco–service functions of the region declined as a whole, but varied spatially, that is, more significantly in the northwest and middle parts of the region and less in the southeast; and (3) the alienation of farmland, forestland and watersurface into construction land and bare land was the major cause of the decline of its eco-service functions, and held responsible for 70% of the decline.
Growth Dynamics of Fecal Coliforms in Biogas Slurry in Storage and After Application in Field
GUO De-Jie, WU Hua-Shan, MA Yan, CHANG Zhi-Zhou
2014, 30(6):  806-810. 
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Biogas slurry produced from anaerobically digested pig waste from an intensive pig farm was used for study. Population of fecal coliforms in the slurry under storage (covered or open) in winter and spring, and summer and autumn was monitored. And the slurries, different in coliform population [104  (Treatment WF), 105 (Treatment LF), 106 (Treatment MF) and 107(Treatment HF)] were top-dressed separately on lettuce in the field. Samples of soil and lettuce were collected from the field 3, 10, 17 and 24 days after the top-dressing for analysis of population of coliforms in the soil, on the surface of and in the lettuce. Results show that coliforms in the slurry lived longer in SA, and open storage reduced the population of coliforms. After 50 days of storage, all the treatments failed to meet Grade III criteria of the “Integrated Standard for Discharge of Wastewater GB 8978—2002”, except for the treatment of open storage in WS. The more the coliforms that survived in the slurry, the more the coliforms that could be found in the soil, on the surface of and in the lettuce that was top-dressed with the slurry. However, the population of coliforms varied with time. During the monitoring period, Treatment WF under open storage was significantly lower than the others in population of coliforms in the soil. On the 3rd day after the top-dressing, Treatment WF was much lower than Treatments HF and MF in number of coliforms on the surface of and in the lettuce, and 24 days after the top-dressing, coliforms were no longer detected in the lettuce in all the treatments, which is up to the “National Standard for Food Safety”, however, they could still be found on the surface of the lettuce with logarithmic value of the population being lower than 3.51, which fails to the “National Standard for Food Safety”. 
Problems in Technological Policy of Rural Human Settlement Environment and the Countermeasures
ZHU Lin, SUN Qin-Fang, JU Chang-Hua, ZHANG Wei-Dong, SHAN Yong-Jie, ZHU Hong-Biao
2014, 30(6):  811-815. 
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Through the analysis on technological policy of the rural human settlement environment,the shortages of the current technological policieshave been pointed out, such as deficiency of pertinence and forward-looking on technological management policies, lack of strict technological evaluation and examination system on evaluation policies, lack of research support and demonstration system on supportive polices. Followingcountermeasuresto deal with the above shortagesare proposed: strengthening system coordination of technological management policies, establishing evaluation and examination technology system, enhancing support toresearch and development, and strengthening the demonstration and extension system and the technology service market.