Abstract: Following the rapid socioeconomic development and advancement of new socialist countryside construction in recent years, rural river regulation has become an increasingly crucial part of water conservancy work. Evaluating the ecological benefits of rural river regulation and identifying problems arising during regulation and after implementation have great significance for future guidance on the comprehensive, centralized regulation of rural rivers. A Status-Response-Benefit (SRB) system is established in accordance with the functions and features of rural rivers based on the project objectives and regulation measures in Pilot Planning for Comprehensive Regulation and Interconnection of Medium and Small-sized Rivers in Key Counties of China. 18 evaluation indicators have been selected for six features, including river connectivity, water quality, riparian zone condition, hydrobiology, river landscape, and management, building a multi-dimensional, multi-level ecological benefit evaluation model, setting evaluation standards, indicator weights and evaluation grades. This system can provide an important reference for the further development of methods to evaluate ecological benefits in comprehensively regulated rural rivers, as well as the practical application of these methods.