Abstract: In June and August 2018, the diversity of amphibians of Yulong Snow Mountain was observed by the application of sample line method. Total number of 1, 143 amphibians which includes number of adults, sub-adults, and juveniles on 11 sampling transects were recorded. These individuals were identified as 8 species, which belong to 2 orders, 6 families and 8 genera. The numbers of individuals vary greatly in different seasons, 705 amphibians were recorded in June, and 438 in August. The numbers of species and individuals of amphibians recorded by each sampling transect were obvious and affected by several factors, such as average water temperature, average air temperature and human interference intensity. It demonstrates that the higher the average water temperature and the average air temperature and the weaker the human interference intensity on the sampling transects, the more the number of species and individuals recorded. The numbers of species and individuals of amphibians were different in each habitat. In artificial channel the number was the largest while in the river it was the smallest. From the results it could be concluded that the habitat type, water temperature, air temperature and human interference are important ecological factors affecting amphibian species diversity.