Abstract: Amphibians, as bioindicators in environmental monitoring, have a critical role in maintaining ecological balance. To reveal the spatial and temporal pattern of amphibian diversity, 15 sampling transects were employed in Yongfeng sample area (Jiangxi Province, China), which are located as stream, paddy field and artificial channel in April, June and August of 2018, to obtain the environmental data, the species and amounts of amphibians. The results showed that: (1) A total of 18 amphibian species were found in the survey which belong to two orders, seven families, and 16 genera, including one endangered and one vulnerable species. (2)Amolops wuyiensis, Fejervarya multistriata and Limnonectes fujianensis were the dominant species in stream, paddy field and artificial channel, respectively. (3) The Pearson correlation analysis show that the relative abundance was negatively related with the air temperature and the water temperature, respectively, and the species richness was negatively related with the water temperature in paddy field; but the relative abundance was positively related with air temperature in artificial channel. (4) The species richness, relative abundance, Simpson index and the Shannon-Wiener index of amphibians in paddy field and artificial channel were higher than those in stream; the relative abundance decreased with month, the interaction between habitat type and month induced the variation of the relative abundance. (5) Cody index in the paddy field vs. artificial channel was lower than that in the paddy field vs. stream and the stream vs. artificial channel. In conclusion, amphibian diversity has significant spatial and temporal variation in Yongfeng sample area of Jiangxi Province.