Abstract: The decline of amphibian populations and their decreasing of biodiversity are attracting increasing research attention, and studies on amphibian diversity are helpful for species conservation and ecological environment monitoring. 12 sampling transects were used which covering three habitat types, i.e., stream, cropland and ditches, at Baiyunshan Mountain and Jiulongshan Mountain in Lishui, Zhejiang Province, China, to investigate and dynamically monitor amphibian diversity in April, June, and August of 2018. The following results were obtained:(1) A total of 20 amphibian species belonging to 7 families in 1 order were recorded in the two sampled areas. Species compositions differed between the two sampling areas, and the dominant species also differed between the sampling areas, between habitat types, and between three months of sampling. (2) Relative abundance of amphibians was negatively correlated with air temperature, and was positively correlated with elevation, as determined by using Pearson's correlation analysis. (3) Relative abundance was higher at Jiulongshan Mountain than at Baiyunshan Mountain, was higher on cropland than in ditches, and was higher in April than in August and species richness was higher in April and June than in August; and the interaction term of habitat type×month interaction showed a significantly effected on species richness, relative abundance, Simpson's diversity index and Shannon-Wiener index. In summary, spatial and temporal structure of amphibian diversity in Lishui of Zhejiang Province, differed significantly between Baiyunshan Mountain and Jiulongshan Mountain.