Abstract: Silicon (Si) and nitrogen (N) play an important role in the growth and development of rice. Appropriate application of silicon and nitrogen fertilizer is of great significance to improve the nutrient management and yield and quality of rice. A field experiment was conducted to explore the effects of different nitrogen fertilizer levels (120, 180, 240 kg·hm^-2, respectively, N1, N2, N3) and silicon fertilizer levels (0, 225, 450 kg·hm^-2, respectively, Si1, Si2, Si3) on nutrient element concentrations in leaves, yield components and quality of two rice cultivars Gui-nong-zhan and Huang-hua-zhan. The results show that Si and N fertilizer application could significantly increase nitrogen concentration in rice leaves at tillering stage and heading stage. At heading stage, compared with N1-Si1, N1-Si3 treatment increased nitrogen concentration in leaves of Gui-nong-zhan and Huang-hua-zhan by 17.0% and 44.4%, respectively. Si and N treatment significantly increased phosphorus concentration in leaves of Huang-hua-zhan at heading stage and Si concentration of two cultivars at tillering and heading stages. N and Si fertilizer significantly improved Si concentration in leaves at tillering and heading stage. Higher N and Si fertilization level resulted in higher yield for both cultivars. Treatments of N2-Si1 and N3-Si3 had the highest yield of Gui-nong-zhan and Huang-hua-zhan, which is 8 188.5 and 8 260.5 kg·hm^-2, respectively. Si application improved head rice rate in grains of both cultivars, while reduced amylase content, which is beneficial to rice quality improvement. The above findings suggested that the combination of low N and high Si, or medium N and low Si treatment are appropriate for plant growth, yield and quality improvement of Gui-nong-zhan.