Abstract: Batch experiments were carried out, to study the washing kinetics characteristics of heavy metals (Cd, Pb and Zn) from a contaminated soil with biodegradable chelating agents of imminodisuccinic acid (IDS) and Dl-2-(2-carboxymethyl) nitrilotriacetic acid (GLDA). The effects of washing time, concentrations and pH value of IDS and GLDA on the heavy metals removal efficiencies were also investigated. Afterwards, fractions of heavy metals in soil before and after washing were determined using the sequential extraction procedure of the optimized European Community Bureau of Reference (BCR). Results show that the removal efficiencies of Cd, Pb and Zn increased with the increasing of washing time (ranging from 5 to 720 min). Both Elovich equation and double-constant equation were suitable for describing the washing kinetic process, which suggested that the desorption of Cd, Pb and Zn from soil as a function of IDS and GLDA was regarded as the heterogeneous diffusion process. The removal efficiencies of Cd, Pb and Zn increased with the increasing of IDS and GLDA concentrations (ranging from 0 to 20 mmol·L^-1), however which increased first and then decreased with the increase of the pH of IDS and GLDA solution (ranging from 3 to 10). The removal efficiencies of Cd and Pb with GLDA were greater than that of IDS, while the removal efficiencies of Zn with IDS were better than that of GLDA. Under the optimal washing conditions (10 mmol·L^-1 IDS and GLDA with pH = 5 for 240 and 360 min, respectively), the removal efficiencies of Cd, Pb and Zn by IDS and GLDA were 21.88% and 66.12%, 17.47% and 22.96%, and 9.21% and 8.11%, respectively. BCR results demonstrate that the acid soluble and reducible fractions of heavy metals with high activity in soil can be effectively removed by using IDS and GLDA, respectively. Soil environmental risk induced by heavy metals could be reduced after washing with IDS and GLDA.