Abstract: Agricultural heritage sites have unique natural and cultural capital endowments. Cultural capital is the cultural resource that these areas have obtained the title of agricultural heritage sites and is also a cultural symbol that is different from other communities. It constitutes a part of the livelihood capital of local farmers, ensuring the compatibility, reciprocity and harmonious coexistence of human and nature. Based on the field survey data of farmers in Aohan Banner, this article added cultural capital to the framework of sustainable livelihood analysis to explore the impact of cultural capital on their pro-environmental behavior in agricultural heritage areas. It draws the following conclusions: (1) In the capital structure of farmers, cultural and human capital scores are relatively high, while financial and material capital scores are relatively low. (2) Cultural capital, human capital, and natural capital of the farmers all positively and significantly affect their pro-environmental level. (3) Under the condition of low dependence on land, and when farmers' income from farming and animal husbandry take a little proportion of all income, the increase of farmers' cultural capital will restrain their pro-environmental behavior. When the natural capital level of farmers is low, the increase of their physical, financial and social capital will negatively affect their pro-environmental behavior. When the degree of dependence on land is high, farmers' cultural capital has a significant positive impact on their pro-environmental behavior. On this basis, it is proposed to pay attention to the power of traditional agricultural culture, enhance farmers' income from agriculture and animal husbandry, actively promote the moderate scale management of agricultural land, to achieve the sustainable promotion role of farmers' cultural capital in pro-environmental behavior.