Abstract: Forests are important ecological resources in the terrestrial biosphere, and clarifying the long-term forest loss status and its influencing factors is of great significance to the protection of forest resources and the coordinated development of social economy. Based on the Global Forest Change dataset, this paper used ArcGIS spatial analysis technology to analyze the spatial and temporal evolution characteristics of forest loss in the 58 counties in Fujian Province from 2001 to 2019, and used the Geographically and Temporally Weighted Regression Model to explore the main influencing factors of forest loss in the counties, and the spatial and temporal differentiation characteristics. The results show that: (1) During 2001-2019, the rate of forest loss in the counties of Fujian Province showed a basic trend of fluctuating increase, and the cumulative area of forest loss amounted to 1 128 900 hm², and the forest loss situation could be divided into three stages: the stage of significant increase, the stage of decline followed by an increase, and the stage of significant decrease. (2) On the whole, forest loss in counties in Fujian Province had strong positive correlation and spatial clustering characteristics. The spatial correlation of forest loss rate among neighboring regions was significant, and the spatial clustering effect showed a basic trend of low in the northwest and high in the southeast. (3) The urbanization rate, per capita GDP, road mileage, population density and timber production all had certain influences on forest loss in the counties in Fujian Province, but there was obvious spatial and temporal heterogeneity. This study effectively reveals the spatio-temporal dynamic characteristics and influencing factors of forest loss in the counties of Fujian Province, which can provide a theoretical basis for the sustainable management of forest resources.