近江牡蛎(<i>Crassostrea hongkongensis</i>)热休克蛋白70对壬基酚的响应

夏欢; 张其中

近江牡蛎(Crassostrea hongkongensis)热休克蛋白70对壬基酚的响应

夏欢,
张其中

Response of HSP70s in Crassostrea hongkongensis to a Pollutant Nonylphenol

摘要

摘要: 以β-actin为内参基因，采用实时荧光定量聚合酶链式反应（PCR）技术，检测不同时间（12、24、48、96和192 h）不同浓度（0.01、0.1、1、10、100、300 μg•L^-1）壬基酚（NP）处理下近江牡蛎（Crassostrea hongkongensis）鳃、外套膜和消化腺中HSP70和HSC70基因mRNA的表达水平。结果显示，0.01 μg•L^-1NP能诱导近江牡蛎鳃和消化腺中HSP70和HSC70基因显著高表达(P<0.05)，随着NP处理浓度的升高，其表达水平逐渐升高，处理一定时间后100 μg•L^-1NP诱导近江牡蛎HSP70和HSC70基因表达量显著高于300 μg•L^-1NP处理组诱导表达量(P<0.05)。HSC70基因在鳃和消化腺中显著高表达峰值出现在96 h时，在外套膜中出现在48 h时；HSP70基因在鳃中显著高表达峰值出现在48 h时，在外套膜和消化腺中出现在24 h时。可见，近江牡蛎HSP70和HSC70基因对NP具有显著反应性。

Abstract: With β-actin as reference genes and by means of the real-time fluorescence quantitative RT-PCR method, levels of mRNA expression of HSC70 and HSP70 in gill, mantle and digestive gland of Crassostrea hongkongensis exposed to NP varying in concentration (0, 0.01, 0.1, 1, 10, 100 and 300 μg•L^-1) were determined at 12, 24, 48, 96 and 192 h. Results show that 0.01 μg•L^-1 NP induced significantly expression of HSC70 and HSP70 mRNA in gill and disgestive gland(P<0.05). With rising NP concentration in treatment, the expression level increased gradually. Within a certain time period, 100 μg•L^-1 NP induced significantly a higher expression level than 300 μg•L^-1 NP did(P<0.05). When the expression of HSC70 gene in gill and digestive gland peaked at 96h, it did at 48h in mantle, and when the expression of HSP70 in gill peaked at 48h, it did at 24h in mantle and digestive gland. It is quite obvious that HSC70 and HSP70 genes in the oysters respond significantly to NP.

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