Abstract: Ecological red line is a new concept recently put forth in China and an important achievement, of idea transformation and active innovation in environmental protection as well. Delineation and protection of ecological red lines is a major step towards realization of ecosystem management. Based on the evaluation of the status quo and sensitivity of regional eco-environments, ecological red line zones of Jiangsu were delineated in line with integrity of the natural ecosystems, consistency of the ecosystem service functions, continuity of the ecological space and importance of the ecosystem service functions. Eventually in the province, a total of 15 types of ecological red line zones were delineated and put under two-level management.