外源脱落酸(ABA)增强菹草抗镉(Cd2+)胁迫能力

    Effects of Exogenous Abscisic Acid on Tolerance of Potamogen crispus to Cd2+ Stress

    • 摘要: 以高等沉水植物菹草(Potamogen crispus)为试验材料,观察4 mg.L-1Cd2+胁迫条件下外施脱落酸(abscisicacid,ABA)对菹草某些生理生化指标的影响。结果表明,外施ABA可以明显减轻Cd2+毒害作用,使Cd2+胁迫下菹草叶片中叶绿素、可溶性蛋白、脯氨酸含量增加,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性提高,过氧化物酶(POD)活性和O2.-产生速率降低,且以施用2.5~5μmol.L-1ABA效果最好。

       

      Abstract: Effects of exogenous abscisic acid(ABA) on the activities of some enzymes,such as superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX),peroxidase(POD),and some other physio-biochemical indices,such as O2.- generation rate,chlorophyll,soluble protein and proline contents,in leaves of Potamogen crispus with or without Cd2+ stress were observed.Results show that Cd2+,4 mg·L-1 in concentration,decreased activities of SOD,CAT and APX,but increased O2.- generation rate,activities of POD and proline content,thus leading to loss of chlorophyll and soluble protein.Exogenous ABA obviously enhanced activities of SOD,CAT and APX,but inhibited O2.- generation and activity of POD.In addition,ABA also increased the loss of chlorophyll and soluble protein triggered by Cd2+ stress,and proline content,thus enhancing the ability of Potamogen crispus to stand Cd2+ stress.The most suitable concentration of ABA is defined to be in the range of 2.5-5 μmol·L-1.

       

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