二氯喹啉酸对湘云鲫的急性毒性及对鱼鳃、肝脏ATP酶活性的影响

Acute Toxicity of Quinclorac to Carassius auratus and Its Effects on Gill and Liver ATPase Activities

摘要: 研究了二氯喹啉酸对湘云鲫的急性毒性和 (1/8～ 1/2 )LC₅₀ 浓度下对湘云鲫鳃、肝脏中Na⁺-K⁺ -ATPase和Mg²⁺ -ATPase活性的影响。结果表明 :(1)在 2 3± 1℃条件下 ,二氯喹啉酸对湘云鲫的 2 4、4 8、72、96h的LC₅₀ 分别为 15 1.6 4、15 0 .92、14 9.6 0、14 9.14mg·L^-1,是一种低毒性农药 ;(2 )鱼鳃和肝脏中Na⁺-K⁺ -ATPase、Mg²⁺ -ATPase活性的抑制随浓度的增加而加强 ,表现出显著的浓度 -效应关系 ,是一种较好的生物指示剂。此外 ,还讨论了二氯喹啉酸抑制ATPase活性的机制。

Abstract: Quinclorac shows acute toxicity to Carassius auratus.Under the concentration of 1/2,1/4,1/6 and 1/8 LC₅₀ of quinclorac,its effects on gill and liver Na⁺-K⁺ -ATPase, and Mg²⁺ -ATPase activities were studied.Results indicated that:(1)At the water temperature of 23±1 ℃,the LC ₅₀ value of 25% EC quinclorac to Carassius auratus at the 24th, 48th,72th and 96th hour was 151.64,150.92,149.60 and 149.14 mg·L^-1 ,respectively,so it is a kind of low toxicity pesticide.(2)The inhibition of Na⁺-K⁺ -ATPase and Mg²⁺ -ATPase in gill and liver increased with the concentration of quinclorac,showing a striking dose response relationship,which can,therefore,be used as a good biomarker for quinclorac pollution.Mechanisms of the inhibition of Na⁺-K⁺ -ATPase、Mg²⁺ -ATPase by quinclorac were also discussed.