Abstract: In order to lay down a solid basis for the study on ecological function of the macrofungi in the Zijin Mountain ecosystem, species composition and ecological characteristics of the macrofungi were investigated and analyzed. Through a two-year field investigation, a total of 290 species of macrofungi were recorded, belonging to 120 genera of 49 families. The habitats they were found in could be sorted into types of evergreen-deciduous broadleaf mix forest, coniferous-broadleaf mix forest, coniferous forest, bamboo groves, and shrubby wasteland. By trophic type, they could be grouped into lignicolous (151 species), entomophilous (151 species), and ectomycorrhizal (49 species), and entomogenous species (2 species). By floral composition, they could be classified into the following distribution types, i.e. Cosmopolitan (18.97%), North Temperate (35.86%), North Temperate-Australia (13.45%), Temperate-subtropical or Tropical (7.59%), Eurasian (5.86%), Eastern Asia-North America (5.17%), China-Japan (2.07%), and China (10.00%).