Abstract: Resources and environment are the heart and base of agricultural sustainable development. Eco-security of agricultural and rural development means the resources and environment in the area are balanced and the agro-ecosystem is healthy. Took Du′an Yao Autonomous County of Guangxi Zhuang Autonomous Region for case study and based on the set evaluation index system eco-security evaluation on agricultural sustainable development in Du′an was conducted, and the county was regionalized according to bio-security. On such a basis, strategies for land use and building-up of eco-security in different eco-security regions for agricultural sustainable development in Du′an have been proposed.