2-氯苯甲酸好氧降解菌的降解特性

    Characteristics of Aerobic Degradation of 2-Chlorobenzoic Acid by Degrading Bacteria

    • 摘要: 分离出 2株以 2 -氯苯甲酸为唯一碳源的细菌W1和W2 ,这 2株菌对 2 -氯苯甲酸的降解均表现为一级动力学反应。W1降解酶系为诱导酶 ,对 2 -氯苯甲酸的降解动力学常数为 -0 .1 34h- 1;W2降解酶系为非诱导酶 ,对 2 -氯苯甲酸的降解动力学常数为 -0 .0 388h- 1。W1还能够降解 4 -氯苯甲酸、苯、甲苯和邻苯二酚 ,但不能降解 3-氯苯甲酸、2 ,4 -二氯苯甲酸、乙苯、丙苯和萘。W1菌体质粒和染色体提取实验表明 ,其降解基因位于染色体上。

       

      Abstract: Two strains of bacteria(W1 and W2)feeding only on 2-chlorobenzoic acid were isolated.The degradation enzyme system of W1 is inducible,with rate constant being -0.134 h -1,whereas the degradation enzyme system of W2 constructive with rate constant being -0.038 8 h -1 while degrading 2-chlorobenzoic acid.Besides,W1 can also degrade 4-chlorobenzoic acid,benzene,toluene and 2-hydroxphenol,but not 3-chlorobenzoic acid,1,4-dichlorobenzoic acid,ethylbenzene,propylbenzene and naphthalene. Plasmid and chromosome extraction experiments indicate that the degradation genes of W1 are on chromosome.

       

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