Culturable Bacterial Community Structure and Influencing Factors in Basomtso Lake, Xizang

This study investigated the culturable bacterial community structure, compositional differences, and their responses to environmental factors in the Basomtso Lake, utilizing traditional pure culture techniques combined with 16S rDNA gene identification. The results show that bacteria isolated from 24 water samples belonged to 4 phyla, 25 genera and 70 species. The culturable bacterial community consisted of Pseudomonadota, Bacteroidota, Bacillota and Actinomycetota, with the predominant genera Pseudomonas, Acinetobacter, Bacillus, Flavobacterium and Brevundimonas. The dominant species are Pseudomonas sp., Pseudomonas umsongensis, Pseudomonas poae. Pearson correlation analysis show that NH₄⁺-N, Temp, and TN were influencing the bacterial diversity index and total abundance. CCA analysis found that NH₄⁺-N, BOD, and Temp had a high degree of explanation of the bacterial community, and among them, NH₄⁺-N was a significant influencing factor of the diversity of bacterial communities in the Basomtso Lake (P< 0.05). In conclusion, the culturable bacterial resources of the Basomtso Lake are rich and highly diverse. The changes of bacterial community structure are jointly influenced by a variety of environmental factors, among which NH₄⁺-N is an important environmental factor influencing the distribution of bacteria in the Basomtso Lake. The study provides a foundation for the development and exploitation of the diversity of bacterial resources in the Basomtso Lake.