ZHANG Lan-he, WANG Jia-jia, GAO Min, QIU Tian-lei, HAN Mei-lin, WANG Xu-ming. Pollution of Antibiotics Resistant Genes in Farmland Amended With Livestock Organic Manure[J]. Journal of Ecology and Rural Environment, 2016, 32(4): 664-669. DOI: 10.11934/j.issn.1673-4831.2016.04.023
    Citation: ZHANG Lan-he, WANG Jia-jia, GAO Min, QIU Tian-lei, HAN Mei-lin, WANG Xu-ming. Pollution of Antibiotics Resistant Genes in Farmland Amended With Livestock Organic Manure[J]. Journal of Ecology and Rural Environment, 2016, 32(4): 664-669. DOI: 10.11934/j.issn.1673-4831.2016.04.023

    Pollution of Antibiotics Resistant Genes in Farmland Amended With Livestock Organic Manure

    • In order to investigate distribution of antibiotics resistant genes (ARGs) in farmland soil amended with organic manure, soil samples from greenhouses and fields in nine vegetable production bases in the suburbs of Beijing were collected for analysis of antibiotics resistant bacteria and 18 types of ARGs. The analysis shows that the soil samples from greenhouses were higher than those from fields in proportion of tetracycline resistant bacteria to total bacteria in count (P<0.05), and little difference was found between the two groups of soil samples in proportion of ampicillin, sulfa methoxazole and ciprofloxacin resistant bacteria to total bacteria in count. Sulfanilamide resistant genes (sulⅠ and sul2) and tetracycline resistance genes (tetL) were detected in all the soil samples, whereas tetracycline resistant genes (tetA, tetA/P and tetC), erythromycin resistant genes (ermB), and I integron (intⅠ1) were detected with a higher detectable rate in greenhouse soil than in field soil, which may be associated with more organic manure applied to the former than to the latter.
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