Abstract: The Chinese giant salamander (Andrias davidianus) is a protected amphibian species, which plays important functional roles in freshwater ecosystems. However, few studies have been conducted on the function zoning of its nature reserves. Zhangjiajie Chinese Giant Salamander Nature Reserve was selected as the model area to adjust the area of the nature reserves and to explore its functional zones. Geographical environment, human activities, and distribution of the Chinese giant salamander were considered comprehensively, and the minimum cost distance, Ripley's K function of spatial statistics, nuclear density analysis, Iso clustering and other methods were incorporated to analyze the geospatial data of the waters in Zhangjiajie. The results show that:(1) The locations and the reaches of the river resulted from clustering method meet with the habitat requirements of Chinese giant salamander, which have been verified by field investigation. (2) The total area of the adjusted nature reserve is 13 101 hm², among which the core zone, buffer zone, and the experimental zone account for 35.46%, 35.73%, and 28.81%, respectively. The adjusted functional zoning take into account the amphibious characteristics of land and water of Chinese giant salamander, and meet the dual needs of ecological and economic development, which has a certain guiding significance for the research on function zoning of amphibians nature reserves.