Abstract: Biodiversity is one of critical indicators to human survival and related to human well-being. Quantitatively assesses of biodiversity's contribution to human well-being is a research hotpot. This paper constructs an indicator system for the evaluation of the biodiversity contributions to human well-being, which is from three aspects namely material contribution, ecological regulation contribution and spiritual culture. The weights of indicators were confirmed by mark experts. This indicator system could be well used to assess the biodiversity contributions to human well-being on multiscale. Furthermore, this indicator system allows to add the characteristic indicators reflecting the local characteristics in the three-levels, which brings the flexibility and promoting delicacy research at the local scale. This paper could provide an important scientific basis for decision making with biodiversity conservation, ecosystem management and human well-being in China.